FastQCFastQC Report
Thu 26 May 2016
SRR1546523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546523_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2070606
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113460.5479555260633843No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35110.16956388612802242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG600.044.0000041
ATTCGAC207.857947E-444.032
CGTCGAT207.857947E-444.040
TAATGCG950.041.684211
CGTTTTT66700.041.5922051
GTATACG501.3496901E-939.61
CGCATCG750.038.1333321
TCGTAAG1100.038.01
CGGTCTA1400.037.71428731
ACGTTAG651.0913936E-1137.230771
AGGGCGA21800.037.0366976
GCGTAAG1550.036.9032251
CACGACG1450.036.41379526
CGTAAGG3150.036.3174632
GGGCGAT39050.036.281697
TAATACG850.036.2352941
TCTAACG553.426976E-936.01
TAAGGGC11500.035.9652184
GGACTAA11050.035.8371058
CGTTAGG2650.035.6981122
Produced by FastQC (version 0.11.5)