##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546523_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2070606
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.412446887529544	31.0	31.0	33.0	30.0	34.0
2	31.80119250113252	31.0	31.0	34.0	30.0	34.0
3	31.94047443115687	33.0	31.0	34.0	30.0	34.0
4	35.61784810823498	37.0	35.0	37.0	33.0	37.0
5	35.51332653339168	37.0	35.0	37.0	33.0	37.0
6	35.54578418105618	37.0	35.0	37.0	33.0	37.0
7	36.00350718581903	37.0	35.0	37.0	35.0	37.0
8	35.99595335858198	37.0	35.0	37.0	35.0	37.0
9	37.686554564219364	39.0	37.0	39.0	35.0	39.0
10	37.05996843436173	39.0	37.0	39.0	33.0	39.0
11	36.83447502808357	39.0	37.0	39.0	32.0	39.0
12	36.36073931979334	38.0	35.0	39.0	32.0	39.0
13	36.18214426114867	39.0	35.0	39.0	32.0	39.0
14	37.228028412938045	39.0	35.0	41.0	32.0	41.0
15	37.39756235614115	39.0	35.0	41.0	32.0	41.0
16	37.469591510891014	39.0	35.0	41.0	32.0	41.0
17	37.402701914318804	39.0	35.0	41.0	32.0	41.0
18	37.3696512035607	39.0	35.0	41.0	32.0	41.0
19	37.34916637931118	39.0	35.0	41.0	32.0	41.0
20	37.20122225087728	39.0	35.0	41.0	32.0	41.0
21	37.07715615621707	39.0	35.0	41.0	32.0	41.0
22	36.998698448666715	39.0	35.0	41.0	32.0	41.0
23	36.90318824537358	39.0	35.0	41.0	31.0	41.0
24	36.84265765674397	38.0	35.0	41.0	31.0	41.0
25	36.6578513729797	38.0	35.0	41.0	31.0	41.0
26	36.53798018551091	38.0	35.0	41.0	31.0	41.0
27	36.44590617432771	38.0	35.0	40.0	31.0	41.0
28	36.504612659289116	38.0	35.0	40.0	31.0	41.0
29	36.41585120491296	38.0	35.0	40.0	31.0	41.0
30	36.3900326764242	38.0	35.0	40.0	31.0	41.0
31	36.27697978273027	38.0	35.0	40.0	30.0	41.0
32	36.0968301067417	38.0	35.0	40.0	30.0	41.0
33	36.00591807422561	38.0	35.0	40.0	30.0	41.0
34	35.93729323685916	38.0	35.0	40.0	30.0	41.0
35	35.725965731771275	38.0	35.0	40.0	29.0	41.0
36	35.59805873256428	38.0	35.0	40.0	28.0	41.0
37	35.543090283714044	38.0	34.0	40.0	28.0	41.0
38	35.51841103522351	38.0	34.0	40.0	28.0	41.0
39	35.43152101365494	38.0	34.0	40.0	28.0	41.0
40	35.31695262159967	38.0	34.0	40.0	27.0	41.0
41	35.33711386907987	37.0	34.0	40.0	27.0	41.0
42	35.1566439969748	37.0	34.0	40.0	27.0	41.0
43	35.17363853866936	37.0	34.0	40.0	27.0	41.0
44	34.82912925008427	37.0	34.0	40.0	26.0	41.0
45	34.739134823331916	37.0	34.0	40.0	26.0	41.0
46	34.76004995638958	36.0	34.0	40.0	26.0	41.0
47	34.68248763888446	36.0	34.0	40.0	26.0	41.0
48	34.6449377621817	36.0	34.0	40.0	26.0	41.0
49	34.62836435323765	36.0	34.0	40.0	26.0	41.0
50	34.0908922315496	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	6.0
14	14.0
15	41.0
16	109.0
17	337.0
18	771.0
19	1466.0
20	2628.0
21	4298.0
22	6509.0
23	9840.0
24	13616.0
25	19534.0
26	26623.0
27	33065.0
28	37837.0
29	43157.0
30	50731.0
31	61652.0
32	76184.0
33	99275.0
34	158890.0
35	245208.0
36	164015.0
37	208030.0
38	300067.0
39	506696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.58919755858913	23.839011381209172	27.725699626099797	13.8460914341019
2	32.39119368919051	25.399230949779923	28.203385868678062	14.006189492351515
3	30.080420900934318	25.54643423229721	30.486340713781374	13.886804152987098
4	25.730003680082064	28.17498838504283	30.200482370861476	15.894525564013625
5	23.16423307959119	32.309719956379915	28.83175263666772	15.694294327361169
6	20.40088746965864	41.072613524736234	27.151519893210008	11.374979112395115
7	86.26909223676546	4.265804310428928	7.157131776880778	2.3079716759248257
8	86.18998496092448	3.6296137459275206	6.896048789581408	3.2843525035665886
9	80.15305664138904	5.508870350032792	9.990698375258257	4.347374633319907
10	43.30176769506126	25.573334569686363	16.20400018158935	14.920897553663034
11	35.964157352968165	24.584348736553455	23.333652080598625	16.117841829879755
12	32.56182006620284	21.39547552745428	27.014120503852496	19.028583902490382
13	23.72411748058298	29.990881896411004	28.049179805332354	18.235820817673666
14	19.636232098235975	32.51323525576571	28.827454378090277	19.02307826790804
15	18.362498708107676	23.064890181908098	39.82186857374121	18.750742536243013
16	20.540170365583794	21.12709998908532	37.58706388371327	20.745665761617612
17	20.66844199234427	21.371714367677868	29.13325857261111	28.82658506736675
18	23.559914343916706	22.889627481036953	31.430750224813416	22.11970795023293
19	27.779548595918296	25.720296377002676	25.595453698096115	20.904701328982917
20	29.07033013523577	24.492008619698773	24.41193544305387	22.025725802011582
21	24.773182343719665	27.49499421908369	27.09680161266798	20.635021824528664
22	24.364799483822612	23.76058023593093	26.181127650552543	25.693492629693914
23	22.543738403153473	28.07622502784209	25.305876637081127	24.07415993192331
24	22.1128500545251	24.348379170155983	31.970109233721917	21.568661541597002
25	22.118983524629986	24.83398579932638	30.0196174453276	23.027413230716032
26	20.62154750831399	28.49078965288423	27.159778345083517	23.727884493718264
27	22.097057576381022	26.982438957483946	27.24086571757254	23.6796377485625
28	21.153855441353883	26.80683819133143	31.47556802211526	20.563738345199425
29	21.768023467525932	24.590675386819125	30.7682871584454	22.873013987209543
30	23.55102805652065	25.717688444832092	29.10360541793079	21.627678080716468
31	27.317075291001764	25.76926754776138	24.364847778862806	22.54880938237405
32	29.256797285432377	25.193011128143162	24.979885115758382	20.570306470666075
33	26.395654219103005	25.849968559928833	25.402949667875006	22.351427553093153
34	23.383830627362233	26.096080084767454	28.02150674730007	22.498582540570247
35	22.440628492335094	25.59632300881964	28.94476303072627	23.018285468118997
36	27.189576384884422	25.4772274396964	25.95452732195309	21.37866885346609
37	22.79443795681071	28.01131649381872	27.221016456051995	21.973229093318576
38	22.614973587442517	29.407188040602605	25.284433639234116	22.693404732720758
39	22.476946362562458	26.726426949405152	25.557542091542285	25.23908459649011
40	23.842730099304262	24.37663176867062	27.63669186701864	24.143946265006477
41	20.743975435210753	24.026154661968523	27.083713656774876	28.146156246045845
42	24.139551416348645	26.016489858524512	25.08275355137578	24.761205173751065
43	22.87195149632523	25.631530093122496	27.060676922601402	24.43584148795087
44	23.335970242528035	27.145386423105123	27.485238620964104	22.033404713402742
45	21.398324934825844	28.450559884400995	26.491374988771405	23.659740192001763
46	23.309166495219273	27.65147014931861	26.990649114317257	22.04871424114486
47	23.15911380533042	25.219476810170548	27.83320438557601	23.78820499892302
48	23.59719811494799	24.575607334277986	28.26795633742006	23.559238213353964
49	23.127383963921673	23.450284602671875	29.01208631675944	24.410245116647012
50	21.439327423952214	27.099409544838565	28.129639342298823	23.331623688910398
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1660.0
1	2018.5
2	2377.0
3	10426.5
4	18476.0
5	12774.5
6	7073.0
7	7325.0
8	7577.0
9	7942.0
10	8307.0
11	8280.0
12	8253.0
13	8197.0
14	8141.0
15	7713.5
16	7286.0
17	6964.5
18	6643.0
19	6676.5
20	6710.0
21	7373.5
22	8037.0
23	8555.5
24	9074.0
25	10044.0
26	11014.0
27	13021.5
28	15029.0
29	18987.0
30	22945.0
31	25815.0
32	28685.0
33	31624.0
34	34563.0
35	41385.5
36	48208.0
37	51673.5
38	55139.0
39	67703.5
40	80268.0
41	100783.0
42	121298.0
43	136788.5
44	152279.0
45	162459.5
46	172640.0
47	177708.0
48	182776.0
49	184785.5
50	186795.0
51	169649.0
52	152503.0
53	139635.0
54	126767.0
55	114781.5
56	102796.0
57	101649.5
58	100503.0
59	96630.5
60	92758.0
61	86715.5
62	80673.0
63	71733.5
64	62794.0
65	52850.5
66	42907.0
67	37454.5
68	32002.0
69	27919.0
70	23836.0
71	20685.0
72	17534.0
73	14006.0
74	10478.0
75	8273.5
76	6069.0
77	4866.5
78	3664.0
79	3033.0
80	2402.0
81	1773.0
82	1144.0
83	728.0
84	312.0
85	213.5
86	115.0
87	76.5
88	38.0
89	43.5
90	49.0
91	27.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2070606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.975302709039173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.13749486998971	18.182079002730482
2	6.811674972537654	3.130005888992774
3	2.3081653266911593	1.5909239124971304
4	1.3017059805946025	1.1962835576931066
5	0.8886591089810091	1.0208606016991857
6	0.6769248369246706	0.9331531823766769
7	0.5351610296212904	0.8606840657541156
8	0.4315713515154928	0.7932385953294079
9	0.37630233177816147	0.7781093984448495
>10	5.218265129528663	29.580954204741378
>50	2.0566081396303217	32.828884048914645
>100	0.25281263396722015	7.629722014742001
>500	0.0031733783455043883	0.47463972591975867
>1k	0.0012693513382017554	0.44897564242916643
>5k	0.0	0.0
>10k+	2.115585563669592E-4	0.5514861577353386
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11346	0.5479555260633843	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3511	0.16956388612802242	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05626372182829568	0.0
2	0.0	0.0	0.0	0.21679643543967322	0.0
3	0.0	0.0	0.0	0.35274697359130613	0.0
4	0.0	0.0	0.0	0.5599326960319829	0.0
5	0.0	0.0	0.0	0.9415601036604743	0.0
6	0.0	0.0	0.0	1.6893122110145533	0.0
7	0.0	0.0	0.0	2.1184136431556753	0.0
8	0.0	0.0	0.0	3.3699313147938335	0.0
9	0.0	0.0	0.0	4.057652687184332	0.0
10	0.0	0.0	0.0	5.019834773008482	0.0
11	0.0	0.0	0.0	5.539489405517032	0.0
12	0.0	0.0	0.0	6.043206674760915	0.0
13	4.8295040195961954E-5	0.0	0.0	6.297963011794614	0.0
14	4.8295040195961954E-5	0.0	0.0	6.413243272742376	0.0
15	9.659008039192391E-5	0.0	0.0	6.531807596423462	0.0
16	9.659008039192391E-5	0.0	0.0	6.748893802104312	0.0
17	9.659008039192391E-5	0.0	0.0	6.963082305373403	0.0
18	9.659008039192391E-5	0.0	0.0	7.328772349737227	0.0
19	9.659008039192391E-5	0.0	0.0	7.475927337214323	0.0
20	9.659008039192391E-5	0.0	0.0	7.65452239585899	0.0
21	9.659008039192391E-5	0.0	0.0	7.834373125548752	0.0
22	9.659008039192391E-5	0.0	0.0	8.01393408499734	0.0
23	9.659008039192391E-5	0.0	0.0	8.228557243628194	0.0
24	9.659008039192391E-5	0.0	0.0	8.395175132304262	0.0
25	9.659008039192391E-5	0.0	0.0	8.53754891080196	0.0
26	9.659008039192391E-5	0.0	0.0	8.673451153913396	0.0
27	9.659008039192391E-5	0.0	0.0	8.822876008279701	0.0
28	9.659008039192391E-5	0.0	0.0	8.958923136511727	0.0
29	9.659008039192391E-5	0.0	0.0	9.11747575347507	0.0
30	9.659008039192391E-5	0.0	0.0	9.343882901913739	0.0
31	9.659008039192391E-5	0.0	0.0	9.521560354794683	0.0
32	9.659008039192391E-5	0.0	0.0	9.708558750433449	0.0
33	9.659008039192391E-5	0.0	0.0	9.886187908274197	0.0
34	9.659008039192391E-5	0.0	0.0	10.053385337432616	0.0
35	9.659008039192391E-5	0.0	0.0	10.254051229446839	0.0
36	9.659008039192391E-5	0.0	0.0	10.423180460213096	0.0
37	9.659008039192391E-5	0.0	0.0	10.609406135208726	0.0
38	9.659008039192391E-5	0.0	0.0	10.803407311675905	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	60	0.0	44.000004	1
ATTCGAC	20	7.857947E-4	44.0	32
CGTCGAT	20	7.857947E-4	44.0	40
TAATGCG	95	0.0	41.68421	1
CGTTTTT	6670	0.0	41.592205	1
GTATACG	50	1.3496901E-9	39.6	1
CGCATCG	75	0.0	38.13333	21
TCGTAAG	110	0.0	38.0	1
CGGTCTA	140	0.0	37.714287	31
ACGTTAG	65	1.0913936E-11	37.23077	1
AGGGCGA	2180	0.0	37.036697	6
GCGTAAG	155	0.0	36.903225	1
CACGACG	145	0.0	36.413795	26
CGTAAGG	315	0.0	36.317463	2
GGGCGAT	3905	0.0	36.28169	7
TAATACG	85	0.0	36.235294	1
TCTAACG	55	3.426976E-9	36.0	1
TAAGGGC	1150	0.0	35.965218	4
GGACTAA	1105	0.0	35.837105	8
CGTTAGG	265	0.0	35.698112	2
>>END_MODULE