FastQCFastQC Report
Thu 26 May 2016
SRR1546522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546522_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2012692
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151960.7550087146965357No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23500.11675904708718472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80400.041.9751241
TACGGGT1900.039.368424
TTACGGG3950.038.4303783
CGGTCTA1150.038.26086831
TAGCATA17300.038.15028830
GTAGCAT17800.037.32584429
GCGATCG1550.036.9032259
GGTACCT19550.036.797958
CATATGC17900.036.748633
GCGTAAG1200.036.6666641
GCGCGAC18300.036.5464489
AGGGTAC12650.036.5217446
GTTGATC18700.036.3529416
TTCGAAT3450.036.34782416
TTGATCC18850.036.1803717
ACCCGCT17100.036.0233934
GCCAGTA18700.035.88235525
GGGTACC21050.035.847987
ATAGGGC14800.035.824324
TCGTAAG800.035.751
Produced by FastQC (version 0.11.5)