##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546522_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2012692
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.357473473338196	31.0	31.0	33.0	30.0	34.0
2	31.75207831103815	31.0	31.0	34.0	30.0	34.0
3	31.8981707086827	31.0	31.0	34.0	30.0	34.0
4	35.588275801762016	37.0	35.0	37.0	33.0	37.0
5	35.478867606171235	37.0	35.0	37.0	33.0	37.0
6	35.512929449712125	37.0	35.0	37.0	33.0	37.0
7	35.968557533889935	37.0	35.0	37.0	35.0	37.0
8	35.95124937148854	37.0	35.0	37.0	35.0	37.0
9	37.63574555868459	39.0	37.0	39.0	35.0	39.0
10	37.0030655460448	39.0	37.0	39.0	33.0	39.0
11	36.80993664206943	39.0	37.0	39.0	32.0	39.0
12	36.45267035393393	39.0	35.0	39.0	32.0	39.0
13	36.33924862820541	39.0	35.0	39.0	32.0	39.0
14	37.41474155012292	40.0	36.0	41.0	32.0	41.0
15	37.561757586356975	40.0	36.0	41.0	32.0	41.0
16	37.59882585114861	40.0	35.0	41.0	32.0	41.0
17	37.52361911310822	39.0	35.0	41.0	32.0	41.0
18	37.45643645426126	39.0	36.0	41.0	32.0	41.0
19	37.431436106468354	39.0	36.0	41.0	32.0	41.0
20	37.27150602277944	39.0	35.0	41.0	32.0	41.0
21	37.16448269283129	39.0	35.0	41.0	32.0	41.0
22	37.08780926242068	39.0	35.0	41.0	32.0	41.0
23	36.984204239893636	39.0	35.0	41.0	31.0	41.0
24	36.90748162162914	39.0	35.0	41.0	31.0	41.0
25	36.71822116846492	38.0	35.0	41.0	31.0	41.0
26	36.6037426491485	38.0	35.0	41.0	31.0	41.0
27	36.52245351002538	38.0	35.0	41.0	30.0	41.0
28	36.53693411609923	38.0	35.0	40.0	31.0	41.0
29	36.4329435402933	38.0	35.0	40.0	30.0	41.0
30	36.36979031068837	38.0	35.0	40.0	30.0	41.0
31	36.27429283765226	38.0	35.0	40.0	30.0	41.0
32	36.088908784851334	38.0	35.0	40.0	30.0	41.0
33	36.022376498739	38.0	35.0	41.0	30.0	41.0
34	35.92386614544103	38.0	35.0	41.0	29.0	41.0
35	35.75715211269285	38.0	35.0	41.0	28.0	41.0
36	35.60733336248169	38.0	35.0	40.0	27.0	41.0
37	35.570753995146795	38.0	35.0	40.0	27.0	41.0
38	35.513566407577514	38.0	35.0	40.0	27.0	41.0
39	35.41303885542348	38.0	34.0	40.0	27.0	41.0
40	35.28818865479666	38.0	34.0	40.0	26.0	41.0
41	35.26681181223953	38.0	34.0	40.0	26.0	41.0
42	35.07682844667738	38.0	34.0	40.0	26.0	41.0
43	35.09457532498763	38.0	34.0	40.0	26.0	41.0
44	34.74869975137776	37.0	34.0	40.0	25.0	41.0
45	34.67528762473344	37.0	34.0	40.0	24.0	41.0
46	34.682832743410316	37.0	34.0	40.0	24.0	41.0
47	34.59843284516459	37.0	34.0	40.0	24.0	41.0
48	34.55174910021007	36.0	34.0	40.0	24.0	41.0
49	34.53522545923569	36.0	34.0	40.0	25.0	41.0
50	34.01269245368889	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	4.0
12	4.0
13	14.0
14	17.0
15	45.0
16	146.0
17	382.0
18	803.0
19	1583.0
20	2858.0
21	4560.0
22	7078.0
23	9863.0
24	14110.0
25	20319.0
26	28159.0
27	34406.0
28	38658.0
29	42716.0
30	49899.0
31	59227.0
32	73637.0
33	93389.0
34	144571.0
35	208306.0
36	161616.0
37	207032.0
38	302164.0
39	507121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.44282980207603	23.02324449046352	26.449799571916614	15.084126135543837
2	33.68334548952349	25.00919166966431	27.899400405029684	13.408062435782526
3	30.075789042734808	25.288817166262895	30.798453017153143	13.836940773849154
4	26.10280162091368	27.95708434276084	30.249784865245154	15.690329171080325
5	23.272761058323876	31.479133419320988	29.42218680255101	15.825918719804122
6	20.157729051439567	41.117021382307875	27.640940591009453	11.084308975243108
7	85.55566375779304	4.135853871332524	7.91313325635517	2.3953491145192607
8	85.04450755505562	3.596725181995059	7.9315165956837905	3.427250667265533
9	78.73996617465563	5.494233593614919	11.21085590840526	4.554944323324184
10	40.421634308677135	24.817508093637773	18.138145329737487	16.622712267947605
11	32.73993238905903	25.87872361990806	25.104238502463367	16.27710548856954
12	29.527866161340132	21.76512849457344	28.92300461272763	19.784000731358798
13	23.599388281962668	26.684460414211415	30.407782214069517	19.308369089756404
14	20.14505945271308	29.981586849850846	30.635884675847073	19.237469021588996
15	19.13785119630823	23.290101018933846	38.088887917276956	19.483159867480964
16	20.996307433029994	21.305942488965027	36.7093921971171	20.988357880887886
17	21.390555534577572	21.656964900739904	30.72745357958396	26.225025985098565
18	23.12440254147182	23.097175325385106	32.130251424460376	21.6481707086827
19	27.561097276682172	25.89030015521501	26.26939442299169	20.279208145111124
20	27.59716836952698	25.24678390931151	25.797588503357694	21.358459217803816
21	25.19232947713808	26.991263442195823	27.715815435247915	20.100591645418177
22	23.835887458190324	23.90802964387994	27.36608482569613	24.889998072233606
23	22.412569831847097	27.718001562086997	25.925824716350043	23.943603889715863
24	22.38598851687193	24.76245744505369	31.501541219421554	21.35001281865283
25	22.934805722882587	24.856759007339424	29.388500575348836	22.819934694429154
26	20.78589272476862	27.658727713927416	28.17569702666876	23.379682534635204
27	22.133739290462724	25.77716809129266	28.194229420100047	23.894863198144574
28	21.969779777531784	26.18184998002675	31.25053411053455	20.59783613190692
29	22.167673941169337	24.894718118817984	30.479278498647584	22.458329441365095
30	23.030796564998518	25.381926295727315	29.2568361179952	22.33044102127896
31	25.668209542244913	25.664781297883632	25.49913250512249	23.167876654748962
32	25.92115435446656	25.547525403787564	26.029765110608082	22.5015551311378
33	24.679782102775786	26.50489990520159	25.45789420338532	23.35742378863731
34	21.270566981932657	27.663596814614454	27.990869939364792	23.074966264088097
35	21.50259453508038	26.792325899839618	28.17539891846343	23.529680646616573
36	24.502854882913034	27.09724090919028	25.387441297525903	23.012462910370786
37	21.856697398310324	28.73658761499524	25.93695408934899	23.469760897345445
38	22.780932204231945	28.249131014581465	24.800118448326916	24.16981833285967
39	21.96123400897902	26.34710129518078	25.125751977947942	26.565912717892253
40	23.364628070266093	24.488496004356357	27.531634249055493	24.61524167632206
41	21.044302854087956	24.096434029647853	27.832574482335104	27.026688633929087
42	23.462655985118438	26.37562031349059	25.671389363101753	24.490334338289216
43	23.033877016453587	25.561139011830917	27.461578820803183	23.943405150912312
44	23.12017934189633	27.062262879765008	27.618781214413335	22.19877656392533
45	21.01772153911279	27.74438413825861	27.640046266393465	23.597848056235133
46	23.687032094329386	26.650078601196803	27.13470317365995	22.528186130813854
47	22.59088822333472	26.101609188092368	27.25787154716171	24.049631041411203
48	22.746301967712895	24.979778326738515	27.917336582050307	24.35658312349828
49	23.168423186458735	23.97371281845409	28.043187929400027	24.81467606568715
50	21.48962682814857	25.90510619607968	28.550766833673507	24.054500142098245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1610.0
1	1983.0
2	2356.0
3	13294.5
4	24233.0
5	16605.5
6	8978.0
7	9076.0
8	9174.0
9	9458.0
10	9742.0
11	9704.5
12	9667.0
13	9319.0
14	8971.0
15	8650.5
16	8330.0
17	8106.5
18	7883.0
19	7513.5
20	7144.0
21	7721.5
22	8299.0
23	8888.0
24	9477.0
25	10335.0
26	11193.0
27	13325.0
28	15457.0
29	18306.0
30	21155.0
31	24831.5
32	28508.0
33	33552.5
34	38597.0
35	43931.0
36	49265.0
37	54990.5
38	60716.0
39	70420.0
40	80124.0
41	92410.5
42	104697.0
43	119996.0
44	135295.0
45	145640.0
46	155985.0
47	166385.0
48	176785.0
49	176662.0
50	176539.0
51	162171.0
52	147803.0
53	131704.5
54	115606.0
55	107487.0
56	99368.0
57	98740.5
58	98113.0
59	94933.0
60	91753.0
61	84082.0
62	76411.0
63	68748.5
64	61086.0
65	53197.5
66	45309.0
67	39724.5
68	34140.0
69	30992.0
70	27844.0
71	23771.0
72	19698.0
73	16273.0
74	12848.0
75	9450.0
76	6052.0
77	4565.5
78	3079.0
79	2517.0
80	1955.0
81	1428.5
82	902.0
83	611.0
84	320.0
85	221.0
86	122.0
87	89.5
88	57.0
89	40.5
90	24.0
91	19.5
92	15.0
93	11.0
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2012692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.709493724844382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89109576163683	20.751943480537957
2	7.624200629720675	4.2252547901239925
3	3.535887895573695	2.9393299036245755
4	2.1879513745889945	2.4250809953775354
5	1.4464164166934341	2.0039733310939307
6	1.0371769845982017	1.7243789487766135
7	0.7357883993301036	1.427182682383556
8	0.6188868427464734	1.3719232868377724
9	0.4979392835742255	1.2417880908198158
>10	6.554091965387858	44.019781036835646
>50	0.8246700661224862	14.172921878954895
>100	0.0421000100123623	2.0722277273344263
>500	0.003071632868989804	0.602602813294755
>1k	5.420528592334949E-4	0.26079744553442824
>5k	0.0	0.0
>10k+	1.8068428641116493E-4	0.7608135884701808
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15196	0.7550087146965357	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2350	0.11675904708718472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.046256456526880416	0.0
2	0.0	0.0	0.0	0.16733807259133537	0.0
3	0.0	0.0	0.0	0.2997478004582917	0.0
4	0.0	0.0	0.0	0.47503542519173325	0.0
5	0.0	0.0	0.0	0.8029047663527256	0.0
6	0.0	0.0	0.0	1.603573721165484	0.0
7	0.0	0.0	0.0	2.077317344134125	0.0
8	0.0	0.0	0.0	3.4613840567757013	0.0
9	0.0	0.0	0.0	4.217833627797994	0.0
10	4.968470088816371E-5	0.0	0.0	5.244717025754562	0.0
11	4.968470088816371E-5	0.0	0.0	5.75125255130939	0.0
12	4.968470088816371E-5	0.0	0.0	6.211978782645333	0.0
13	4.968470088816371E-5	0.0	0.0	6.472425984701087	0.0
14	4.968470088816371E-5	0.0	0.0	6.595246565296628	0.0
15	4.968470088816371E-5	0.0	0.0	6.701273716991969	0.0
16	9.936940177632742E-5	0.0	0.0	6.893205716522945	0.0
17	9.936940177632742E-5	0.0	0.0	7.084293076138823	0.0
18	9.936940177632742E-5	0.0	0.0	7.412559894906921	0.0
19	9.936940177632742E-5	0.0	0.0	7.542634441832133	0.0
20	1.4905410266449114E-4	0.0	0.0	7.701774538776922	0.0
21	1.4905410266449114E-4	0.0	0.0	7.865634682306085	0.0
22	1.4905410266449114E-4	0.0	0.0	8.028501131817487	0.0
23	1.4905410266449114E-4	0.0	0.0	8.211489885188593	0.0
24	1.4905410266449114E-4	0.0	0.0	8.362779799393051	0.0
25	1.4905410266449114E-4	0.0	0.0	8.492953715720041	0.0
26	1.9873880355265484E-4	0.0	0.0	8.620643397002622	0.0
27	1.9873880355265484E-4	0.0	0.0	8.763635966158757	0.0
28	1.9873880355265484E-4	0.0	0.0	8.899821731293214	0.0
29	1.9873880355265484E-4	0.0	0.0	9.04629222951152	0.0
30	1.9873880355265484E-4	0.0	0.0	9.255464820250689	0.0
31	1.9873880355265484E-4	0.0	0.0	9.417933792154985	0.0
32	1.9873880355265484E-4	0.0	0.0	9.59635155304438	0.0
33	1.9873880355265484E-4	0.0	0.0	9.763192778626834	0.0
34	1.9873880355265484E-4	0.0	0.0	9.920742965143202	0.0
35	1.9873880355265484E-4	0.0	0.0	10.100899690563683	0.0
36	1.9873880355265484E-4	0.0	0.0	10.262325283749327	0.0
37	1.9873880355265484E-4	0.0	0.0	10.430657050358425	0.0
38	1.9873880355265484E-4	0.0	0.0	10.620055130144106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8040	0.0	41.975124	1
TACGGGT	190	0.0	39.36842	4
TTACGGG	395	0.0	38.430378	3
CGGTCTA	115	0.0	38.260868	31
TAGCATA	1730	0.0	38.150288	30
GTAGCAT	1780	0.0	37.325844	29
GCGATCG	155	0.0	36.903225	9
GGTACCT	1955	0.0	36.79795	8
CATATGC	1790	0.0	36.7486	33
GCGTAAG	120	0.0	36.666664	1
GCGCGAC	1830	0.0	36.546448	9
AGGGTAC	1265	0.0	36.521744	6
GTTGATC	1870	0.0	36.35294	16
TTCGAAT	345	0.0	36.347824	16
TTGATCC	1885	0.0	36.18037	17
ACCCGCT	1710	0.0	36.02339	34
GCCAGTA	1870	0.0	35.882355	25
GGGTACC	2105	0.0	35.84798	7
ATAGGGC	1480	0.0	35.82432	4
TCGTAAG	80	0.0	35.75	1
>>END_MODULE