FastQCFastQC Report
Thu 26 May 2016
SRR1546521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546521_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1671970
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128710.7698104631063954No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25260.15107926577629982No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.857509E-444.0000041
CCGTCTA207.857509E-444.00000415
CGTACCG207.857509E-444.00000434
CGGTCTA502.7284841E-1144.031
TTGCGAG1600.042.6250041
CGTTTTT87600.041.8652951
TAGTCGG603.6379788E-1240.3333322
CGACGGT557.8216544E-1140.028
CTAGACG501.3478711E-939.61
TATAGCG900.039.1111071
ACGGGAT7150.038.1538475
TATTGCG1500.038.133331
GCCGGAT357.292194E-637.71428719
CGATCGC357.292194E-637.71428710
CGTTAGG2950.037.2881362
CGAGGGA9200.036.8260884
TCGCCGT301.3013485E-436.66666425
AGGGCGA17250.036.220296
GGGCGAT35050.036.216837
TAATGCG553.426976E-936.01
Produced by FastQC (version 0.11.5)