FastQCFastQC Report
Thu 26 May 2016
SRR1546520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546520_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1463309
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85680.5855222649488249No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21870.14945578821697947No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA207.8571873E-444.00000438
CGTTGAC207.8571873E-444.00000437
AAATGCG408.314601E-944.0000041
TATACGA254.4434375E-544.034
CGTTTTT48150.041.8068541
TTAGGCG452.3517714E-839.111111
ATTGGGC3600.038.54
ACTACGG750.038.133332
CGTTAGG2200.038.02
TTAGTAG2200.038.01
TAGCCGT1700.037.5294144
CGCATCG1350.037.48148321
TAAGGGA13050.037.4252854
TAAGGGC7650.037.385624
CCAGTAG11750.037.25957526
TGATCCT12000.036.8518
TATTAGG4300.036.8372082
TTCGAAT2150.036.83720816
GCGCGAC8900.036.8314639
ACGGGAT4800.036.6666645
Produced by FastQC (version 0.11.5)