##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546520_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1463309
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.392560969692664	31.0	31.0	33.0	30.0	34.0
2	31.793051911797168	31.0	31.0	34.0	30.0	34.0
3	31.934247653776474	33.0	31.0	34.0	30.0	34.0
4	35.628232997951905	37.0	35.0	37.0	33.0	37.0
5	35.515307429941316	37.0	35.0	37.0	33.0	37.0
6	35.55027612076465	37.0	35.0	37.0	33.0	37.0
7	35.9895346779115	37.0	35.0	37.0	35.0	37.0
8	35.97609322432924	37.0	35.0	37.0	35.0	37.0
9	37.67096354905218	39.0	37.0	39.0	35.0	39.0
10	37.06903736668058	39.0	37.0	39.0	33.0	39.0
11	36.816442733557984	39.0	37.0	39.0	32.0	39.0
12	36.33504816822694	38.0	35.0	39.0	32.0	39.0
13	36.14630539414437	38.0	35.0	39.0	32.0	39.0
14	37.17209010537077	39.0	35.0	41.0	32.0	41.0
15	37.35892009138193	39.0	35.0	41.0	32.0	41.0
16	37.43061718338369	39.0	35.0	41.0	32.0	41.0
17	37.35348924936565	39.0	35.0	41.0	32.0	41.0
18	37.3278227633398	39.0	35.0	41.0	32.0	41.0
19	37.292550650614466	39.0	35.0	41.0	32.0	41.0
20	37.14204245309774	39.0	35.0	41.0	32.0	41.0
21	37.03465091788542	39.0	35.0	41.0	32.0	41.0
22	36.959864936250646	39.0	35.0	41.0	32.0	41.0
23	36.87363024487651	38.0	35.0	41.0	31.0	41.0
24	36.79553600777416	38.0	35.0	41.0	31.0	41.0
25	36.5986309111746	38.0	35.0	41.0	31.0	41.0
26	36.4932136684733	38.0	35.0	40.0	31.0	41.0
27	36.404605589113444	38.0	35.0	40.0	31.0	41.0
28	36.45480892962457	38.0	35.0	40.0	31.0	41.0
29	36.354705670504316	38.0	35.0	40.0	30.0	41.0
30	36.332975468612574	38.0	35.0	40.0	30.0	41.0
31	36.2298140720791	38.0	35.0	40.0	30.0	41.0
32	36.07213035660958	38.0	35.0	40.0	30.0	41.0
33	36.01283324301293	38.0	35.0	40.0	30.0	41.0
34	35.91896311715434	38.0	35.0	40.0	30.0	41.0
35	35.73377119938441	38.0	35.0	40.0	29.0	41.0
36	35.568550456533785	38.0	35.0	40.0	28.0	41.0
37	35.52879467016194	38.0	34.0	40.0	28.0	41.0
38	35.474272351225885	38.0	34.0	40.0	28.0	41.0
39	35.37165219376085	38.0	34.0	40.0	28.0	41.0
40	35.236768857432025	37.0	34.0	40.0	27.0	41.0
41	35.203059640855074	37.0	34.0	40.0	27.0	41.0
42	35.02459835892488	37.0	34.0	40.0	27.0	41.0
43	35.02505485854321	37.0	34.0	40.0	27.0	41.0
44	34.67711125948108	36.0	34.0	40.0	26.0	41.0
45	34.59244014763799	36.0	34.0	40.0	25.0	41.0
46	34.607378892633065	36.0	34.0	40.0	26.0	41.0
47	34.512610801956384	36.0	33.0	40.0	26.0	41.0
48	34.467428957246895	36.0	34.0	40.0	26.0	41.0
49	34.4609723578547	36.0	34.0	40.0	26.0	41.0
50	33.95214954599473	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	6.0
13	8.0
14	12.0
15	37.0
16	85.0
17	267.0
18	588.0
19	1094.0
20	1909.0
21	3093.0
22	4757.0
23	6829.0
24	9927.0
25	13718.0
26	19113.0
27	23419.0
28	27110.0
29	30450.0
30	36112.0
31	43889.0
32	55403.0
33	70783.0
34	116363.0
35	176172.0
36	117742.0
37	150500.0
38	211837.0
39	342082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.59187977385501	24.226052050523847	27.936136523454717	14.245931652166425
2	31.974654703825372	25.54914922275473	28.984035497629	13.492160575790896
3	29.699195453591827	26.056970879014617	30.43396849195898	13.80986517543458
4	26.424357398198197	27.997094256920445	29.652178726434403	15.926369618446959
5	23.42458086432872	32.187801756156766	28.801367312030475	15.586250067484036
6	21.110852185013552	40.874893819418865	26.420393778757596	11.593860216809983
7	86.08373214406527	4.466589079955089	7.155973208666112	2.293705567313534
8	86.06405072339471	3.5855038136169464	7.010276025091078	3.3401694378972593
9	79.87861757154504	5.600047563433288	10.060896229026133	4.4604386359955415
10	44.97669323430663	25.186273029141486	15.46850323479183	14.368530501760052
11	36.55810221901184	24.126414858379196	22.593655885393993	16.72182703721497
12	32.66507620741757	20.31259289733064	27.54524164069243	19.477089254559356
13	24.83446763465543	28.526237452240093	28.31623396015469	18.32306095294979
14	20.297626817029077	31.472846814992593	29.2524682073301	18.97705816064823
15	19.046968206988407	22.206314592475	39.191243954626124	19.555473245910466
16	21.78418912205146	20.036574640079436	36.50015136925967	21.679084868609433
17	21.47290831943219	20.181110073128778	29.108821171741578	29.23716043569745
18	24.547378578277044	21.521086797115306	31.688180691842938	22.243353932764716
19	28.21311151643296	24.8550374527868	25.26117176891552	21.67067926186472
20	28.99941160752787	23.652899011760333	24.914696759194403	22.432992621517396
21	25.926649805338446	26.07754069714599	26.718963663860468	21.27684583365509
22	24.940118594227194	22.81568691233362	25.77883413551068	26.465360357928503
23	22.805231157602392	26.988079756223737	24.9675905772465	25.23909850892737
24	22.996236611679418	23.220317786605563	31.78522102987134	21.99822457184368
25	22.95892391832484	24.007642951693732	29.595936333337665	23.43749679664377
26	21.511451101578682	27.18113535828728	26.689919900718166	24.617493639415873
27	22.460942972400225	25.781567666159365	27.028330995025655	24.72915836641475
28	21.144132920661324	26.203351445251823	31.151725302038052	21.5007903320488
29	22.043191151014586	24.662733571651646	30.02079533440989	23.27327994292388
30	23.17118257319541	25.655346888456233	28.998591548333263	22.174878990015095
31	27.675631052634813	25.02014270396751	24.21361448607232	23.090611757325348
32	27.70877511175015	25.713707767805705	24.53992970725937	22.037587413184774
33	26.902110217322523	25.19447362108755	24.218329826441305	23.685086335148625
34	22.46975860874224	26.553106691751367	27.291023290364507	23.686111409141883
35	23.568569591248327	24.718702611683522	28.453047169121493	23.25968062794666
36	28.10014836237596	24.67038745746797	24.936975033981206	22.29248914617487
37	23.84902983580365	27.238744516708362	26.359162692227002	22.553062955260987
38	24.830435676948614	27.287674715319866	24.554349081431194	23.32754052630032
39	23.95932779747818	25.040234154235367	25.38561575169701	25.61482229658944
40	25.710017501429977	22.309095344865643	27.36236844029525	24.61851871340913
41	21.649904428934697	22.913410633024196	27.566631518018408	27.8700534200227
42	24.727791601090406	25.21907539692573	24.472343161970574	25.580789840013285
43	23.419865523959736	23.96787008075533	27.155781861520705	25.45648253376423
44	23.520391113565214	25.60928689702585	27.35990826271143	23.510413726697504
45	21.84603525297801	27.55221214384658	26.33756780010237	24.264184803073036
46	24.372364278494835	25.563021890796815	26.794819139361543	23.269794691346803
47	23.199952983272844	25.536643320036983	27.339748474177362	23.923655222512814
48	24.25113219422555	23.61387786175032	27.784698925517443	24.350291018506688
49	23.5270882636545	22.459917898406967	28.686285671720736	25.3267081662178
50	22.45745772082315	25.273404318568392	27.900805639820437	24.36833232078802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1055.0
1	1390.5
2	1726.0
3	7756.0
4	13786.0
5	9455.5
6	5125.0
7	5292.5
8	5460.0
9	5665.5
10	5871.0
11	5874.0
12	5877.0
13	5745.5
14	5614.0
15	5430.0
16	5246.0
17	4983.5
18	4721.0
19	4540.5
20	4360.0
21	4515.5
22	4671.0
23	4930.5
24	5190.0
25	5633.5
26	6077.0
27	7822.0
28	9567.0
29	11403.0
30	13239.0
31	14632.5
32	16026.0
33	18428.5
34	20831.0
35	24372.5
36	27914.0
37	31297.5
38	34681.0
39	42572.5
40	50464.0
41	63522.5
42	76581.0
43	87528.0
44	98475.0
45	107657.0
46	116839.0
47	121252.5
48	125666.0
49	127021.0
50	128376.0
51	117278.5
52	106181.0
53	98979.5
54	91778.0
55	85768.5
56	79759.0
57	78512.5
58	77266.0
59	74490.0
60	71714.0
61	68854.5
62	65995.0
63	59297.5
64	52600.0
65	44976.0
66	37352.0
67	32984.5
68	28617.0
69	25166.5
70	21716.0
71	18336.0
72	14956.0
73	12306.5
74	9657.0
75	7727.0
76	5797.0
77	4512.5
78	3228.0
79	2591.5
80	1955.0
81	1340.0
82	725.0
83	527.0
84	329.0
85	237.5
86	146.0
87	95.0
88	44.0
89	34.5
90	25.0
91	17.5
92	10.0
93	8.5
94	7.0
95	6.5
96	6.0
97	7.0
98	8.0
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1463309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.644268041933937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55711158927491	19.888953581535468
2	6.476438500716994	3.321670497389747
3	2.5318493206260193	1.9478226785976593
4	1.500235029477444	1.5388971688727293
5	1.0560434600297897	1.3540730776467638
6	0.7965543792233298	1.225623240646762
7	0.6534897637037083	1.1730786664154578
8	0.567149822405201	1.1635313652555364
9	0.4889229101675043	1.128426314416008
>10	7.210470314022263	45.676772108476335
>50	1.0960997741534706	17.525407808404033
>100	0.06163299375882078	2.6808166183190796
>500	0.003201713952449569	0.5443601995391352
>1k	5.336189920749282E-4	0.2443326134432192
>5k	2.668094960374641E-4	0.5862340610421456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8568	0.5855222649488249	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2187	0.14945578821697947	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06116274826437888	0.0
2	0.0	0.0	0.0	0.22066426161528427	0.0
3	0.0	0.0	0.0	0.3619194578862018	0.0
4	0.0	0.0	0.0	0.5596220620525125	0.0
5	0.0	0.0	0.0	0.9559840061121745	0.0
6	0.0	0.0	0.0	1.6818730698710935	0.0
7	0.0	0.0	0.0	2.094089491693142	0.0
8	0.0	0.0	0.0	3.2045179794561505	0.0
9	0.0	0.0	0.0	3.842250679794903	0.0
10	0.0	0.0	0.0	4.773222880471589	0.0
11	0.0	0.0	0.0	5.28726331895724	0.0
12	0.0	0.0	0.0	5.776223613741185	0.0
13	0.0	0.0	0.0	6.026751697693379	0.0
14	0.0	0.0	0.0	6.136571291504392	0.0
15	0.0	0.0	0.0	6.249329430762744	0.0
16	0.0	0.0	0.0	6.462886512691441	0.0
17	0.0	0.0	0.0	6.693596499440651	0.0
18	0.0	0.0	0.0	7.078067585178523	0.0
19	0.0	0.0	0.0	7.2199378258453955	0.0
20	0.0	0.0	0.0	7.3976856562762885	0.0
21	0.0	0.0	0.0	7.575775178038268	0.0
22	0.0	0.0	0.0	7.752156243144817	0.0
23	0.0	0.0	0.0	7.963731515353216	0.0
24	0.0	0.0	0.0	8.129315134397451	0.0
25	0.0	0.0	0.0	8.267290093889944	0.0
26	0.0	0.0	0.0	8.399729653818845	0.0
27	0.0	0.0	0.0	8.546656926185788	0.0
28	0.0	0.0	0.0	8.690440638306741	0.0
29	0.0	0.0	0.0	8.840443132653458	0.0
30	0.0	0.0	0.0	9.062337483060652	0.0
31	0.0	0.0	0.0	9.222932408671033	0.0
32	0.0	0.0	0.0	9.405600594269563	0.0
33	0.0	0.0	0.0	9.578974775662557	0.0
34	0.0	0.0	0.0	9.743259967648665	0.0
35	0.0	0.0	0.0	9.944106132060966	0.0
36	0.0	0.0	0.0	10.108391324047075	0.0
37	0.0	0.0	0.0	10.283132270764412	0.0
38	0.0	0.0	0.0	10.474479416172525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAA	20	7.8571873E-4	44.000004	38
CGTTGAC	20	7.8571873E-4	44.000004	37
AAATGCG	40	8.314601E-9	44.000004	1
TATACGA	25	4.4434375E-5	44.0	34
CGTTTTT	4815	0.0	41.806854	1
TTAGGCG	45	2.3517714E-8	39.11111	1
ATTGGGC	360	0.0	38.5	4
ACTACGG	75	0.0	38.13333	2
CGTTAGG	220	0.0	38.0	2
TTAGTAG	220	0.0	38.0	1
TAGCCGT	170	0.0	37.52941	44
CGCATCG	135	0.0	37.481483	21
TAAGGGA	1305	0.0	37.425285	4
TAAGGGC	765	0.0	37.38562	4
CCAGTAG	1175	0.0	37.259575	26
TGATCCT	1200	0.0	36.85	18
TATTAGG	430	0.0	36.837208	2
TTCGAAT	215	0.0	36.837208	16
GCGCGAC	890	0.0	36.831463	9
ACGGGAT	480	0.0	36.666664	5
>>END_MODULE