FastQCFastQC Report
Thu 26 May 2016
SRR1546519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546519_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1172128
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60510.5162405471074831No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26850.22907054519642908No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA207.8565435E-444.00000420
TCTAGCG408.312782E-944.0000041
CTCGACT207.8565435E-444.00000439
ACCGTTG207.8565435E-444.00000422
GTCCCGA207.8565435E-444.00000439
TACCGTT207.8565435E-444.00000413
GTACGAC207.8565435E-444.00000444
CGTTTTT38800.041.845361
CGTTAGG2450.040.408162
CGACGGT1200.040.33333228
CACGACG1200.040.33333226
TAATGCG557.8216544E-1140.01
TTTCGCG501.3478711E-939.6000021
ACGTAGG1400.039.2857132
ACGTTAG900.039.111111
TCTAACG452.3510438E-839.111111
AACGGGA3000.038.8666654
GGCGATA5550.038.8468488
TCGTTAG850.038.823531
CGGTCTA1250.038.7231
Produced by FastQC (version 0.11.5)