##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546519_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1172128
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.531979442518224	31.0	31.0	33.0	30.0	34.0
2	31.921732950667504	31.0	31.0	34.0	30.0	34.0
3	32.05804741461684	33.0	31.0	34.0	30.0	34.0
4	35.714158351306345	37.0	35.0	37.0	33.0	37.0
5	35.61396536896994	37.0	35.0	37.0	33.0	37.0
6	35.65110209806438	37.0	35.0	37.0	33.0	37.0
7	36.08038029976248	37.0	35.0	37.0	35.0	37.0
8	36.088645608670724	37.0	35.0	37.0	35.0	37.0
9	37.821138135084226	39.0	38.0	39.0	35.0	39.0
10	37.19129224794562	39.0	37.0	39.0	33.0	39.0
11	36.97664418903055	39.0	37.0	39.0	33.0	39.0
12	36.42688511834885	38.0	35.0	39.0	32.0	39.0
13	36.186037702366974	39.0	35.0	39.0	32.0	39.0
14	37.255915736165335	39.0	35.0	41.0	32.0	41.0
15	37.471972344317344	39.0	35.0	41.0	33.0	41.0
16	37.54457533648202	39.0	35.0	41.0	33.0	41.0
17	37.49168179584482	39.0	35.0	41.0	33.0	41.0
18	37.462726767042504	39.0	35.0	41.0	33.0	41.0
19	37.437899273799445	39.0	35.0	41.0	33.0	41.0
20	37.30132459936116	39.0	35.0	41.0	32.0	41.0
21	37.203914589532886	39.0	35.0	41.0	32.0	41.0
22	37.12865830352999	39.0	35.0	41.0	32.0	41.0
23	37.064610690982555	39.0	35.0	41.0	32.0	41.0
24	37.00477166316307	39.0	35.0	41.0	32.0	41.0
25	36.854885302629064	38.0	35.0	41.0	32.0	41.0
26	36.767763418329736	38.0	35.0	41.0	32.0	41.0
27	36.65886746157416	38.0	35.0	41.0	31.0	41.0
28	36.75497897840509	38.0	35.0	41.0	32.0	41.0
29	36.71310983100822	38.0	35.0	41.0	31.0	41.0
30	36.710216802260504	38.0	35.0	40.0	31.0	41.0
31	36.60120140462475	38.0	35.0	40.0	31.0	41.0
32	36.42547656911191	38.0	35.0	41.0	31.0	41.0
33	36.377132019711155	38.0	35.0	41.0	31.0	41.0
34	36.29758268721505	38.0	35.0	41.0	30.0	41.0
35	36.14083956700975	38.0	35.0	41.0	30.0	41.0
36	35.98840826257883	38.0	35.0	41.0	30.0	41.0
37	35.95845846187447	38.0	35.0	41.0	30.0	41.0
38	35.90812692811706	38.0	35.0	40.0	30.0	41.0
39	35.841542050015015	38.0	35.0	40.0	30.0	41.0
40	35.72934355292255	38.0	35.0	40.0	29.0	41.0
41	35.74449718802042	38.0	35.0	40.0	29.0	41.0
42	35.584231414999046	38.0	35.0	40.0	29.0	41.0
43	35.602588625133095	38.0	35.0	40.0	29.0	41.0
44	35.379049045837995	38.0	34.0	40.0	28.0	41.0
45	35.30562617734582	37.0	34.0	40.0	28.0	41.0
46	35.31211778918343	37.0	34.0	40.0	28.0	41.0
47	35.237366567473856	37.0	34.0	40.0	28.0	41.0
48	35.22341587266919	37.0	34.0	40.0	28.0	41.0
49	35.2203820743127	37.0	34.0	40.0	28.0	41.0
50	34.69803041988588	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	2.0
14	10.0
15	31.0
16	47.0
17	134.0
18	310.0
19	633.0
20	1193.0
21	1930.0
22	3026.0
23	4393.0
24	6387.0
25	9512.0
26	13262.0
27	16494.0
28	18663.0
29	20985.0
30	25482.0
31	31454.0
32	39876.0
33	52600.0
34	89575.0
35	155521.0
36	84268.0
37	105095.0
38	163719.0
39	327520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.24252982609408	23.86266687597259	29.350548745529498	13.544254552403833
2	31.032276338420377	25.925410876627808	29.58550601982036	13.456806765131454
3	29.00067228152557	25.292459526604606	31.66428922438505	14.042578967484781
4	25.323002265964128	28.465321193589777	31.03202039367714	15.179656146768952
5	22.850405416473286	32.83131193862786	29.781815637882552	14.536467007016299
6	20.403317726391656	41.07435365420841	27.05088522755194	11.47144339184799
7	87.8058539681673	3.9955533866608426	6.403566845941739	1.7950257992301184
8	87.89970037402058	3.23710379753747	5.918807502252314	2.944388326189631
9	81.80975115345764	5.566627535559256	8.928888312539245	3.6947329984438557
10	41.921530754320344	29.70605599388463	14.909207868082666	13.46320538371236
11	36.38263056594502	23.644516639820907	25.70299489475552	14.269857899478556
12	33.59044404706653	22.22735059652188	27.588710448005678	16.59349490840591
13	22.65964126784788	33.53132081137896	27.97689330858063	15.832144612192526
14	17.80402822899888	36.700599251958835	28.612318791121787	16.8830537279205
15	16.27868287422534	24.239844112588386	43.099047203035845	16.382425810150426
16	18.628596876791615	21.623150372655548	40.7387247809113	19.00952796964154
17	18.372822763384203	21.826285183870702	29.715952523956428	30.084939528788663
18	21.68696592863578	23.21103155969314	34.033057823036394	21.068944688634687
19	26.618679871140355	25.237346091894402	27.629576292009062	20.51439774495618
20	27.44248068470338	24.70071528024243	26.457946572387996	21.398857462666193
21	22.86695650986923	28.17132599852576	28.907423080073162	20.054294411531846
22	22.95406303748396	24.74738253842584	27.2808942368069	25.0176601872833
23	19.751255835540146	29.52552963498867	26.375702994894755	24.34751153457643
24	19.919496846760765	25.605224002839282	35.1654426820279	19.309836468372055
25	19.201486527068717	25.894526877610634	33.0307782085233	21.873208386797348
26	17.822968139998363	31.642277976466733	28.53067241802943	22.00408146550547
27	19.406754211144175	29.470416200278464	29.639339730814385	21.483489857762976
28	17.7928519752109	27.807372573643836	35.48136381009583	18.918411641049442
29	18.520161620573862	24.89113816921019	34.77069057304322	21.818009637172732
30	20.312116082885147	27.982950667503893	31.848142864943078	19.856790384667885
31	26.758852275519395	25.919950722105433	26.1966269895438	21.124570012831363
32	26.600593109284993	26.26786494307789	28.154007070900107	18.97753487673701
33	26.646065958666632	26.168643697616645	26.69588986868328	20.48940047503344
34	20.651328182587566	27.055236288186958	29.86346201097491	22.429973518250566
35	21.810075350132408	25.82985817248628	30.23458188866745	22.12548458871386
36	27.345136367359196	25.567514810669138	27.462870949247865	19.624477872723798
37	22.604015943651206	29.23980998662262	28.58587116765404	19.57030290207213
38	21.759483605886047	31.03568893499686	25.550537142701142	21.654290316415956
39	21.2865830352999	29.109448797400965	27.00438859919736	22.599579568101777
40	23.157368478527943	25.854002293264898	27.621983264626387	23.36664596358077
41	19.311798711403533	24.59791080837588	28.3086830107292	27.781607469491387
42	22.609732042916814	25.977879548991233	26.911480657402603	24.500907750689343
43	22.331861366676677	26.253787982199896	27.80029143574763	23.614059215375796
44	20.875877037320155	28.839938982773212	29.221467280024026	21.062716699882607
45	19.282876955417837	31.255972044008846	26.967959130743402	22.493191869829914
46	22.826517240437905	28.69191760626826	28.232411477244806	20.24915367604903
47	21.72305413743209	25.961072510852055	28.928154604275303	23.38771874744055
48	22.6357530918125	24.694999180976822	29.78667858800404	22.88256913920664
49	22.093576810723743	22.91464754702558	31.021697289033277	23.970078353217396
50	20.877839280351633	28.593037620464663	28.705738622403015	21.823384476780696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1174.0
1	1312.5
2	1451.0
3	5723.5
4	9996.0
5	6949.5
6	3903.0
7	4203.5
8	4504.0
9	4850.5
10	5197.0
11	5218.5
12	5240.0
13	5015.0
14	4790.0
15	4564.5
16	4339.0
17	4195.5
18	4052.0
19	4021.5
20	3991.0
21	4766.0
22	5541.0
23	6216.0
24	6891.0
25	7471.5
26	8052.0
27	9913.5
28	11775.0
29	14071.5
30	16368.0
31	18404.0
32	20440.0
33	23747.5
34	27055.0
35	30502.0
36	33949.0
37	38034.5
38	42120.0
39	49153.5
40	56187.0
41	70474.0
42	84761.0
43	94738.0
44	104715.0
45	109905.0
46	115095.0
47	113959.0
48	112823.0
49	109438.5
50	106054.0
51	95343.0
52	84632.0
53	75975.5
54	67319.0
55	58907.0
56	50495.0
57	46717.0
58	42939.0
59	39712.0
60	36485.0
61	32610.5
62	28736.0
63	25375.5
64	22015.0
65	17816.0
66	13617.0
67	11306.5
68	8996.0
69	7886.0
70	6776.0
71	5634.5
72	4493.0
73	3378.5
74	2264.0
75	1816.0
76	1368.0
77	1064.5
78	761.0
79	598.0
80	435.0
81	343.0
82	251.0
83	146.5
84	42.0
85	31.0
86	20.0
87	13.5
88	7.0
89	8.0
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1172128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.311402477390192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24027540796187	18.23890549976853
2	6.623768304593162	3.0881865773070336
3	2.325986739693008	1.6266603913816895
4	1.3375619565195986	1.2472178042749538
5	0.9588791541895039	1.1176408945245504
6	0.7220588936225347	1.0099323288968387
7	0.5912313920277026	0.9647703055777807
8	0.4676989397879692	0.8722174578916825
9	0.42466757373940744	0.8909637057482496
>10	6.298597730470081	37.09622862909496
>50	1.9052635728139093	29.25453638084618
>100	0.10107011310900436	3.4288334986385935
>500	0.0018376384201637004	0.3261679900381562
>1k	7.350553680654802E-4	0.3193139214269
>5k	3.675276840327401E-4	0.5184246145838938
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6051	0.5162405471074831	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2685	0.22907054519642908	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08736247235796774	0.0
2	0.0	0.0	0.0	0.3236847852794234	0.0
3	0.0	0.0	0.0	0.4873187911217887	0.0
4	0.0	0.0	0.0	0.7076019001337738	0.0
5	0.0	0.0	0.0	1.0862294902945753	0.0
6	0.0	0.0	0.0	1.7228493816375003	0.0
7	0.0	0.0	0.0	2.0671803762046466	0.0
8	0.0	0.0	0.0	3.2318142728439216	0.0
9	0.0	0.0	0.0	3.7984759343689425	0.0
10	0.0	0.0	0.0	4.601374593901007	0.0
11	0.0	0.0	0.0	5.10362349504491	0.0
12	0.0	0.0	0.0	5.534037238253843	0.0
13	0.0	0.0	0.0	5.74868956291463	0.0
14	0.0	0.0	0.0	5.8473136039749924	0.0
15	0.0	0.0	0.0	5.957284528652161	0.0
16	0.0	0.0	0.0	6.179188621037976	0.0
17	0.0	0.0	0.0	6.41917947527915	0.0
18	0.0	0.0	0.0	6.776904911408993	0.0
19	0.0	0.0	0.0	6.927741680089547	0.0
20	0.0	0.0	0.0	7.122856889349968	0.0
21	0.0	0.0	0.0	7.319081192497747	0.0
22	0.0	0.0	0.0	7.522898523028202	0.0
23	0.0	0.0	0.0	7.745741079472549	0.0
24	0.0	0.0	0.0	7.917138742526413	0.0
25	0.0	0.0	0.0	8.057652406563106	0.0
26	0.0	0.0	0.0	8.208574490158071	0.0
27	0.0	0.0	0.0	8.390124628026973	0.0
28	0.0	0.0	0.0	8.54684812580196	0.0
29	0.0	0.0	0.0	8.708093314040788	0.0
30	0.0	0.0	0.0	8.918735837724208	0.0
31	0.0	0.0	0.0	9.102162903710175	0.0
32	0.0	0.0	0.0	9.268100412241667	0.0
33	0.0	0.0	0.0	9.432502252313741	0.0
34	0.0	0.0	0.0	9.610810423434984	0.0
35	0.0	0.0	0.0	9.819831963744575	0.0
36	0.0	0.0	0.0	9.996092576919926	0.0
37	0.0	0.0	0.0	10.173718228725873	0.0
38	0.0	0.0	0.0	10.358936907914494	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	20	7.8565435E-4	44.000004	20
TCTAGCG	40	8.312782E-9	44.000004	1
CTCGACT	20	7.8565435E-4	44.000004	39
ACCGTTG	20	7.8565435E-4	44.000004	22
GTCCCGA	20	7.8565435E-4	44.000004	39
TACCGTT	20	7.8565435E-4	44.000004	13
GTACGAC	20	7.8565435E-4	44.000004	44
CGTTTTT	3880	0.0	41.84536	1
CGTTAGG	245	0.0	40.40816	2
CGACGGT	120	0.0	40.333332	28
CACGACG	120	0.0	40.333332	26
TAATGCG	55	7.8216544E-11	40.0	1
TTTCGCG	50	1.3478711E-9	39.600002	1
ACGTAGG	140	0.0	39.285713	2
ACGTTAG	90	0.0	39.11111	1
TCTAACG	45	2.3510438E-8	39.11111	1
AACGGGA	300	0.0	38.866665	4
GGCGATA	555	0.0	38.846848	8
TCGTTAG	85	0.0	38.82353	1
CGGTCTA	125	0.0	38.72	31
>>END_MODULE