FastQCFastQC Report
Thu 26 May 2016
SRR1546518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546518_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1297858
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131041.0096636149717457No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.856859E-444.01
TATCGCG254.4431585E-544.01
CGTTTTT76600.042.621411
ACGGGTA950.039.3684235
GCGATAA1700.038.8235329
ACGGGAT5900.038.0338975
CGGGATC3900.037.7948726
TCCGATC357.290935E-637.71428715
CGCATCG357.290935E-637.71428721
AACGGGA5750.037.4956554
GGCGATA6800.037.2058838
GGGCGAT28250.037.0690277
TGGGCGA8000.036.8500026
AGGGCGA14150.036.692586
TAAGACG601.9826984E-1036.6666641
ATAGGGC6250.036.6079984
TAGGGAT20850.036.5083925
CGTTCGA553.425157E-936.014
TCGACGG553.425157E-936.02
GTAGGGA16150.035.9628454
Produced by FastQC (version 0.11.5)