##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546518_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1297858
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.450282696566187	31.0	31.0	33.0	30.0	34.0
2	31.84793097549963	31.0	31.0	34.0	30.0	34.0
3	31.95937999380518	33.0	31.0	34.0	30.0	34.0
4	35.647156314481244	37.0	35.0	37.0	33.0	37.0
5	35.53682375113456	37.0	35.0	37.0	33.0	37.0
6	35.57322989109748	37.0	35.0	37.0	33.0	37.0
7	36.04091664881674	37.0	35.0	37.0	35.0	37.0
8	36.03741934787935	37.0	35.0	37.0	35.0	37.0
9	37.725157914039904	39.0	38.0	39.0	35.0	39.0
10	37.10453377796338	39.0	37.0	39.0	33.0	39.0
11	36.894761214246856	39.0	37.0	39.0	32.0	39.0
12	36.63359165640617	39.0	35.0	39.0	32.0	39.0
13	36.559765398063576	39.0	35.0	39.0	32.0	39.0
14	37.67074132917469	40.0	36.0	41.0	32.0	41.0
15	37.777488754547875	40.0	36.0	41.0	33.0	41.0
16	37.801149278272355	40.0	36.0	41.0	33.0	41.0
17	37.7247557128746	40.0	36.0	41.0	33.0	41.0
18	37.623084343587664	39.0	36.0	41.0	33.0	41.0
19	37.57961425672146	39.0	36.0	41.0	33.0	41.0
20	37.40757078201159	39.0	35.0	41.0	32.0	41.0
21	37.31129753794329	39.0	35.0	41.0	32.0	41.0
22	37.25069152403422	39.0	35.0	41.0	32.0	41.0
23	37.14097613144119	39.0	35.0	41.0	32.0	41.0
24	37.08794952914726	39.0	35.0	41.0	32.0	41.0
25	36.92033103775606	39.0	35.0	41.0	31.0	41.0
26	36.83276829976777	39.0	35.0	41.0	31.0	41.0
27	36.71966809928359	39.0	35.0	41.0	31.0	41.0
28	36.77268930807531	39.0	35.0	41.0	31.0	41.0
29	36.709913565274476	39.0	35.0	41.0	31.0	41.0
30	36.67869597444405	39.0	35.0	41.0	31.0	41.0
31	36.548499142433144	39.0	35.0	41.0	31.0	41.0
32	36.308006731090764	39.0	35.0	41.0	30.0	41.0
33	36.17413384206901	39.0	35.0	41.0	30.0	41.0
34	36.02539646093795	39.0	35.0	41.0	29.0	41.0
35	35.821817178766864	39.0	35.0	41.0	28.0	41.0
36	35.69087296144879	38.0	35.0	41.0	27.0	41.0
37	35.63480519440493	38.0	35.0	41.0	27.0	41.0
38	35.577573201382585	38.0	35.0	41.0	27.0	41.0
39	35.463000574793234	38.0	35.0	41.0	26.0	41.0
40	35.35902772106039	38.0	35.0	40.0	25.0	41.0
41	35.34829234014815	38.0	35.0	40.0	26.0	41.0
42	35.16673395702766	38.0	34.0	40.0	25.0	41.0
43	35.22026831903028	38.0	34.0	40.0	25.0	41.0
44	34.98369004929661	38.0	34.0	40.0	24.0	41.0
45	34.90319511071319	38.0	34.0	40.0	24.0	41.0
46	34.90370287042188	38.0	34.0	40.0	24.0	41.0
47	34.81582422730376	38.0	34.0	40.0	24.0	41.0
48	34.780076094611275	38.0	34.0	40.0	24.0	41.0
49	34.74818970950597	37.0	34.0	40.0	24.0	41.0
50	34.23246071604135	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	9.0
14	12.0
15	29.0
16	83.0
17	224.0
18	519.0
19	928.0
20	1792.0
21	2838.0
22	4160.0
23	6199.0
24	8794.0
25	13260.0
26	19234.0
27	23988.0
28	26147.0
29	27049.0
30	30122.0
31	35101.0
32	43531.0
33	55449.0
34	84519.0
35	123989.0
36	98534.0
37	124474.0
38	192161.0
39	374706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.52092447709996	23.20870233877666	26.77457780435148	16.495795379771902
2	34.58074766268729	25.019301032932724	27.13663590315736	13.26331540122263
3	28.554356485840515	25.24490352565535	32.65688542197991	13.543854566524228
4	25.005432027232565	27.828314037437067	32.17262597294928	14.993627962381092
5	22.777530361564978	31.57333082663897	31.23569758787171	14.413441223924343
6	20.251753273470598	40.65660495986464	28.578087895594123	10.513553871070641
7	85.16709840367744	3.6715110589910447	9.281986164896313	1.879404372435197
8	85.22765972856816	3.0870865687925795	9.111936745006002	2.5733169576332697
9	78.57492884429575	6.166776334545074	11.456029858428272	3.8022649627308995
10	35.58347677480895	34.74131992868249	17.201573669846777	12.473629626661777
11	28.179585131809485	25.441997506660975	30.872868988749154	15.505548372780382
12	26.344561577614805	23.141591761194213	32.558646631603764	17.95520002958721
13	21.869495738362748	27.86483575244749	33.392713224405135	16.87295528478462
14	18.88611851219471	30.735026482095883	32.76621941691618	17.61263558879323
15	18.050587968791657	25.29344504560591	40.01354539556716	16.642421590035273
16	20.459942459036352	24.30088653766437	37.19382243666102	18.04534856663826
17	20.54246304295231	23.19421693282316	32.44176173356407	23.82155829066046
18	22.339423881503215	23.431993330549258	34.58783626560071	19.640746522346824
19	24.646841179851727	25.670990200776973	30.476600675882874	19.205567943488425
20	25.83572316848222	25.233885371126885	29.583436708792487	19.346954751598403
21	23.59950009939454	26.628567994341445	31.191470869694527	18.58046103656949
22	22.990650749157457	24.535349784028764	30.598108575822625	21.87589089099116
23	19.9541090011388	28.108699102675335	30.53230784877853	21.404884047407343
24	19.94933189917541	25.725695723260944	34.49075322569958	19.834219151864072
25	20.075925101205215	26.721490332532525	32.89712742071937	20.30545714554289
26	19.64105472247349	28.692276042525457	30.728477229404138	20.938192005596914
27	19.99355861735259	27.34235948770975	31.48117898876456	21.182902906173094
28	19.09384539757046	26.85378523690573	34.73546412627576	19.316905239248054
29	19.907493731979926	25.19019800317138	33.48394046189953	21.418367802949167
30	20.513646331108642	26.617703939876318	32.48051789949285	20.388131829522184
31	23.254084807428857	26.3780783413902	29.20173085191138	21.166105999269565
32	23.64087596640002	27.35630554344158	29.390349329433572	19.612469160724824
33	22.42625926719256	27.182865922157895	29.11543481644371	21.27543999420584
34	20.147042280434377	27.79510547378835	30.600651226867654	21.45720101890962
35	20.11314026650065	28.119023806918786	30.51890114326837	21.248934783312194
36	23.817628739045414	27.182711822094557	28.6707790836902	20.328880355169826
37	21.592654974581194	29.333794606189585	29.079760651781626	19.993789767447595
38	21.789903055650157	29.74608932564271	27.730845747377604	20.73316187132953
39	21.530013298835467	28.798682136258357	28.213487145743215	21.457817419162957
40	23.049671073414814	26.922359765089865	28.80530843898177	21.222660722513556
41	20.37580382445537	26.85139668592404	29.156579533354186	23.616219956266402
42	22.353061737108373	27.73531464921432	27.837560041237175	22.074063572440128
43	22.017200649069466	26.770802352799766	28.98799406406556	22.224002934065208
44	21.12858263384746	28.296007729659177	29.5845924592675	20.99081717722586
45	20.15004723166941	29.196876699916324	28.515831470006738	22.13724459840753
46	21.813865615498766	28.493409910791474	29.06519819579646	20.6275262779133
47	21.58202207021107	27.48829224768811	29.449523753754264	21.480161928346554
48	22.186787768769772	25.965552471842066	29.86636442507578	21.98129533431238
49	21.4527321170729	25.6243749316181	30.479836777212917	22.44305617409609
50	20.09765321013547	28.02409816790435	30.215632218624844	21.662616403335342
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1004.0
1	1286.0
2	1568.0
3	11608.0
4	21648.0
5	14963.5
6	8279.0
7	8338.0
8	8397.0
9	8467.0
10	8537.0
11	8561.5
12	8586.0
13	8249.0
14	7912.0
15	7481.5
16	7051.0
17	6889.0
18	6727.0
19	6721.5
20	6716.0
21	7013.5
22	7311.0
23	8016.5
24	8722.0
25	10454.5
26	12187.0
27	14116.0
28	16045.0
29	19380.5
30	22716.0
31	26947.0
32	31178.0
33	34439.5
34	37701.0
35	42242.5
36	46784.0
37	50898.5
38	55013.0
39	61641.0
40	68269.0
41	75435.5
42	82602.0
43	90297.5
44	97993.0
45	100938.5
46	103884.0
47	106227.5
48	108571.0
49	107502.5
50	106434.0
51	97633.0
52	88832.0
53	79695.5
54	70559.0
55	63552.5
56	56546.0
57	51313.5
58	46081.0
59	42463.0
60	38845.0
61	35510.0
62	32175.0
63	27869.5
64	23564.0
65	20451.0
66	17338.0
67	14213.5
68	11089.0
69	9639.5
70	8190.0
71	6746.5
72	5303.0
73	4309.5
74	3316.0
75	2566.5
76	1817.0
77	1480.5
78	1144.0
79	932.0
80	720.0
81	502.0
82	284.0
83	202.5
84	121.0
85	87.0
86	53.0
87	43.0
88	33.0
89	21.0
90	9.0
91	5.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1297858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.38010949401036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.81167404610633	21.97975174904817
2	6.406551190647171	3.7645035092039265
3	2.7905033041379173	2.459558778569091
4	1.7869930935760219	2.1000821101721527
5	1.3905336774493735	2.0427015849285737
6	1.0649275063640111	1.8772612044094834
7	0.9354104411102383	1.923772282716153
8	0.8169250864251348	1.920107879005947
9	0.7335856657756437	1.9397544465352405
>10	9.09703450074761	55.0287181962114
>50	0.14611845583926325	2.6118689924712264
>100	0.01763710844284325	0.9633510841900776
>500	0.001579442533777592	0.27494485289765563
>1k	2.632404222962653E-4	0.10015571997256373
>5k	0.0	0.0
>10k+	2.632404222962653E-4	1.0134676096683206
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13104	1.0096636149717457	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04823331982389445	0.0
2	0.0	0.0	0.0	0.17806262318373814	0.0
3	0.0	0.0	0.0	0.3111280278736195	0.0
4	0.0	0.0	0.0	0.4679248423171102	0.0
5	0.0	0.0	0.0	0.7829824218057754	0.0
6	0.0	0.0	0.0	1.3919858721061935	0.0
7	0.0	0.0	0.0	1.7469553679986562	0.0
8	0.0	0.0	0.0	2.6819575022845332	0.0
9	0.0	0.0	0.0	3.248891635294462	0.0
10	0.0	0.0	0.0	4.000899944369877	0.0
11	0.0	0.0	0.0	4.400481408597859	0.0
12	0.0	0.0	0.0	4.801526823427524	0.0
13	0.0	0.0	0.0	5.034449069158567	0.0
14	0.0	0.0	0.0	5.1461716150765335	0.0
15	0.0	0.0	0.0	5.255736760107808	0.0
16	0.0	0.0	0.0	5.440040435856619	0.0
17	0.0	0.0	0.0	5.629660563790492	0.0
18	0.0	0.0	0.0	5.965059351639393	0.0
19	0.0	0.0	0.0	6.105829759496031	0.0
20	0.0	0.0	0.0	6.272643078056305	0.0
21	0.0	0.0	0.0	6.42296768983972	0.0
22	0.0	0.0	0.0	6.585543256658278	0.0
23	0.0	0.0	0.0	6.767535431457063	0.0
24	0.0	0.0	0.0	6.912543591055416	0.0
25	0.0	0.0	0.0	7.048151646790327	0.0
26	0.0	0.0	0.0	7.1744366486934625	0.0
27	0.0	0.0	0.0	7.317210357373457	0.0
28	0.0	0.0	0.0	7.452202012855027	0.0
29	0.0	0.0	0.0	7.5900445195082975	0.0
30	7.705003166756301E-5	0.0	0.0	7.793148402983993	0.0
31	7.705003166756301E-5	0.0	0.0	7.948943567015806	0.0
32	7.705003166756301E-5	0.0	0.0	8.109284682916005	0.0
33	7.705003166756301E-5	0.0	0.0	8.266697897612836	0.0
34	7.705003166756301E-5	0.0	0.0	8.425806213006354	0.0
35	7.705003166756301E-5	0.0	0.0	8.605255736760109	0.0
36	7.705003166756301E-5	0.0	0.0	8.765596852660307	0.0
37	7.705003166756301E-5	0.0	0.0	8.928018319415528	0.0
38	7.705003166756301E-5	0.0	0.0	9.100995640509208	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.856859E-4	44.0	1
TATCGCG	25	4.4431585E-5	44.0	1
CGTTTTT	7660	0.0	42.62141	1
ACGGGTA	95	0.0	39.368423	5
GCGATAA	170	0.0	38.823532	9
ACGGGAT	590	0.0	38.033897	5
CGGGATC	390	0.0	37.794872	6
TCCGATC	35	7.290935E-6	37.714287	15
CGCATCG	35	7.290935E-6	37.714287	21
AACGGGA	575	0.0	37.495655	4
GGCGATA	680	0.0	37.205883	8
GGGCGAT	2825	0.0	37.069027	7
TGGGCGA	800	0.0	36.850002	6
AGGGCGA	1415	0.0	36.69258	6
TAAGACG	60	1.9826984E-10	36.666664	1
ATAGGGC	625	0.0	36.607998	4
TAGGGAT	2085	0.0	36.508392	5
CGTTCGA	55	3.425157E-9	36.0	14
TCGACGG	55	3.425157E-9	36.0	2
GTAGGGA	1615	0.0	35.962845	4
>>END_MODULE