FastQCFastQC Report
Thu 26 May 2016
SRR1546517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546517_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1258304
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65450.5201445755556686No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21100.1676860281776105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG454.802132E-1044.021
TAATTCG302.5279096E-644.041
ACGAACC207.8567665E-444.017
CGTTTTT42600.041.36621
CACAACG1400.040.8571412
CTAAGCG404.1249587E-738.51
GGACCGA6900.038.2608688
ACAACGA1500.038.1333313
GACCGAT6550.037.9541979
GCGATCT1750.037.7142879
CTAGGGA7000.037.7142874
ACGCACC357.2907605E-637.71428310
GCGATAC700.037.7142839
GACGGGT1350.037.481484
ACGGGTC950.037.052635
TGTTACG950.037.052631
GGCGATC4250.036.752948
TGGGCGA7850.036.7133756
GGGCGAT27450.036.7067387
CGTAAGG2100.036.6666682
Produced by FastQC (version 0.11.5)