##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546517_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1258304
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.507120695793702	31.0	31.0	33.0	30.0	34.0
2	31.90108670082905	31.0	31.0	34.0	30.0	34.0
3	32.03847798306292	33.0	31.0	34.0	30.0	34.0
4	35.70805703550175	37.0	35.0	37.0	33.0	37.0
5	35.605481664208334	37.0	35.0	37.0	33.0	37.0
6	35.64434429199939	37.0	35.0	37.0	33.0	37.0
7	36.05173551065561	37.0	35.0	37.0	35.0	37.0
8	36.04212733965719	37.0	35.0	37.0	35.0	37.0
9	37.74614401607243	39.0	38.0	39.0	35.0	39.0
10	37.178301904786125	39.0	37.0	39.0	33.0	39.0
11	36.92940100325517	39.0	37.0	39.0	33.0	39.0
12	36.48262343599003	39.0	35.0	39.0	32.0	39.0
13	36.33573047530644	39.0	35.0	39.0	32.0	39.0
14	37.39982627409593	40.0	35.0	41.0	32.0	41.0
15	37.56264066553074	40.0	35.0	41.0	33.0	41.0
16	37.62923744977366	40.0	35.0	41.0	33.0	41.0
17	37.578094005900006	39.0	35.0	41.0	33.0	41.0
18	37.518998588576366	39.0	36.0	41.0	33.0	41.0
19	37.522163960378414	39.0	36.0	41.0	33.0	41.0
20	37.38563733406236	39.0	35.0	41.0	32.0	41.0
21	37.27191044453487	39.0	35.0	41.0	32.0	41.0
22	37.20536134352271	39.0	35.0	41.0	32.0	41.0
23	37.12461456055134	39.0	35.0	41.0	32.0	41.0
24	37.09310389222318	39.0	35.0	41.0	32.0	41.0
25	36.92646768984284	39.0	35.0	41.0	32.0	41.0
26	36.83877266542902	38.0	35.0	41.0	32.0	41.0
27	36.72054924724073	38.0	35.0	41.0	31.0	41.0
28	36.78887693276029	38.0	35.0	41.0	31.0	41.0
29	36.72590725293729	38.0	35.0	41.0	31.0	41.0
30	36.715652179441534	38.0	35.0	41.0	31.0	41.0
31	36.61191333731753	38.0	35.0	41.0	31.0	41.0
32	36.42337384288693	38.0	35.0	41.0	30.0	41.0
33	36.36108841742536	38.0	35.0	41.0	30.0	41.0
34	36.29408870988251	38.0	35.0	41.0	30.0	41.0
35	36.12361957046946	38.0	35.0	41.0	30.0	41.0
36	35.99294923961141	38.0	35.0	41.0	30.0	41.0
37	35.953539844107624	38.0	35.0	41.0	30.0	41.0
38	35.91696283251106	38.0	35.0	41.0	30.0	41.0
39	35.846576026143126	38.0	35.0	40.0	29.0	41.0
40	35.7492672676873	38.0	35.0	40.0	29.0	41.0
41	35.768166516199585	38.0	35.0	40.0	29.0	41.0
42	35.59590607675093	38.0	35.0	40.0	28.0	41.0
43	35.62476953105132	38.0	35.0	40.0	29.0	41.0
44	35.39479966685316	38.0	34.0	40.0	28.0	41.0
45	35.29637194191547	38.0	34.0	40.0	27.0	41.0
46	35.29725646584609	38.0	34.0	40.0	28.0	41.0
47	35.202886583846194	37.0	34.0	40.0	27.0	41.0
48	35.173704446620214	37.0	34.0	40.0	27.0	41.0
49	35.168820889069735	37.0	34.0	40.0	27.0	41.0
50	34.6236473856874	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	9.0
15	24.0
16	47.0
17	156.0
18	344.0
19	714.0
20	1288.0
21	2190.0
22	3357.0
23	4995.0
24	7396.0
25	10490.0
26	14795.0
27	18395.0
28	20727.0
29	23193.0
30	27492.0
31	33601.0
32	42248.0
33	55820.0
34	93422.0
35	148742.0
36	91695.0
37	118209.0
38	182619.0
39	356326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.190701134225115	23.85226463557296	27.53190008137938	13.425134148822544
2	32.786433167183766	25.47834227658817	28.273135903565432	13.462088652662633
3	30.38645669091094	24.958833477442653	30.439941381414986	14.214768450231421
4	25.856708712679925	28.220684349727886	30.137232338131327	15.78537459946086
5	23.376068104369054	32.49818803723107	29.058796602410865	15.066947255989014
6	20.949786379126188	41.383878617567774	26.731378108946647	10.934956894359392
7	86.43992230812268	4.51258201515691	6.881882279639896	2.1656133970805147
8	86.68763669192818	3.7999561314276993	6.736766314022684	2.7756408626214335
9	80.57869958293068	6.318743324347693	9.180452418493463	3.9221046742281676
10	41.9556800264483	30.530460047810386	15.081172753166166	12.432687172575148
11	33.96373213468287	24.84653946899954	26.026143125985453	15.163585270332131
12	31.34091602665175	23.861086033263824	27.828092416458976	16.96990552362545
13	22.9084545547022	32.32684629469508	28.70037765118763	16.064321499415087
14	18.312347413661563	35.56708076903515	29.273768501093535	16.846803316209755
15	17.42480354508926	25.451321779156704	40.55156782462743	16.572306851126594
16	19.529541350897716	23.117783937744775	38.67086173134632	18.681812980011188
17	20.24987602360002	22.464603148364784	29.638147856161943	27.647372971873253
18	22.780266136005288	23.85965553634098	32.805109099231984	20.554969228421747
19	26.704357611515185	25.371690783785155	27.98743387925334	19.936517725446315
20	28.001579904379227	24.490663623416918	26.766266339453743	20.741490132750116
21	24.381707441127105	27.25080743604089	28.85455343064951	19.51293169218249
22	23.37495549565129	25.38067112557856	27.792568409541733	23.45180496922842
23	20.51793525253039	29.35141269518336	27.31621293423529	22.814439118050963
24	20.436476399979657	25.242548700473016	33.89260464879711	20.428370250750216
25	19.83105831341234	26.77016046996592	32.105437159859626	21.293344056762116
26	19.543290015767255	30.513453028838818	28.536426809419662	21.406830145974265
27	20.262114719495447	29.64379037180204	28.981629240628653	21.112465668073852
28	18.865155002288795	28.29340127663903	33.40003687503179	19.441406846040383
29	19.68792914907685	25.659300137327705	33.181965566349625	21.470805147245816
30	21.39928030110371	27.371366537815984	30.5783816947256	20.65097146635471
31	25.433122679416105	26.93498550429785	26.79527363816693	20.836618178119117
32	26.450285463608157	27.614471542647884	27.08502873709374	18.850214256650222
33	25.25931730329078	26.8779245714867	26.944045318142518	20.918712807080006
34	21.71764533848736	27.631955393927065	28.909309673973855	21.74108959361172
35	21.702625120797517	26.60350757845481	30.27487793092925	21.418989369818423
36	26.42843064950918	27.04314696607497	26.813949570215144	19.7144728142007
37	23.25701897156808	28.989258557550478	27.987592823355882	19.766129647525556
38	21.667736890290424	31.142712730786837	26.208769899801638	20.9807804791211
39	21.471838283912316	29.222667972127564	27.062538146584608	22.242955597375516
40	23.072008036213823	26.898666776867913	27.185481409897765	22.8438437770205
41	19.534707034230202	25.837238060119017	28.01119602258278	26.616858883068
42	22.794809521387517	27.320345480901274	26.065322847261076	23.81952215045013
43	22.41421786786023	26.659376430496923	28.02875934591323	22.897646355729616
44	21.832800340776156	29.01564327857179	27.947062077208688	21.204494303443365
45	20.353825466659885	30.30436206195005	26.921475255582116	22.420337215807944
46	22.27617491480596	29.533244748486855	27.52498601291898	20.665594323788213
47	22.524763491175424	27.002457275825236	28.269162301001984	22.203616931997356
48	22.68712489191801	25.76992523269417	29.41840763440313	22.12454224098469
49	22.31972559890138	24.874275214892428	29.892776308427855	22.913222877778345
50	20.0004132546666	30.060064976349114	28.123013198718276	21.81650857026601
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1037.0
1	1289.5
2	1542.0
3	6353.0
4	11164.0
5	7857.0
6	4550.0
7	4768.0
8	4986.0
9	5181.0
10	5376.0
11	5325.0
12	5274.0
13	5153.5
14	5033.0
15	4800.5
16	4568.0
17	4732.5
18	4897.0
19	4918.0
20	4939.0
21	5640.0
22	6341.0
23	6563.5
24	6786.0
25	8205.5
26	9625.0
27	11481.0
28	13337.0
29	16123.5
30	18910.0
31	20512.0
32	22114.0
33	26097.0
34	30080.0
35	35529.0
36	40978.0
37	44029.0
38	47080.0
39	54592.0
40	62104.0
41	75033.5
42	87963.0
43	97103.5
44	106244.0
45	112101.5
46	117959.0
47	115667.5
48	113376.0
49	110908.0
50	108440.0
51	101070.0
52	93700.0
53	83660.5
54	73621.0
55	65115.0
56	56609.0
57	52009.5
58	47410.0
59	43167.5
60	38925.0
61	36255.0
62	33585.0
63	28887.5
64	24190.0
65	20479.5
66	16769.0
67	13474.0
68	10179.0
69	8614.5
70	7050.0
71	6149.5
72	5249.0
73	4014.5
74	2780.0
75	2132.0
76	1484.0
77	1261.0
78	1038.0
79	797.0
80	556.0
81	418.5
82	281.0
83	186.5
84	92.0
85	78.5
86	65.0
87	37.0
88	9.0
89	7.0
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1258304.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.655734805226857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04268166246796	19.241996625585507
2	6.436825101696214	3.1740930539009846
3	2.326787384282297	1.7210595808503533
4	1.3499926197231935	1.331402400836341
5	0.9512574565374341	1.1726975789940792
6	0.7248522821702886	1.0723059385292462
7	0.6081295355767309	1.0495716389483953
8	0.4928342362798134	0.9720952186121272
9	0.4497331425697384	0.9979650986688675
>10	7.1659950401362025	44.94942792415323
>50	1.4030757869848784	21.788393618205156
>100	0.045896464349302175	1.619239788445187
>500	0.0012928581506801114	0.22002662807647932
>1k	3.2321453767002786E-4	0.16814783963830907
>5k	3.2321453767002786E-4	0.5215770665557976
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6545	0.5201445755556686	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2110	0.1676860281776105	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0720016784497228	0.0
2	0.0	0.0	0.0	0.2143361222725192	0.0
3	0.0	0.0	0.0	0.3249612176389807	0.0
4	0.0	0.0	0.0	0.4624478663343675	0.0
5	0.0	0.0	0.0	0.7353548903921469	0.0
6	0.0	0.0	0.0	1.2460422918468033	0.0
7	0.0	0.0	0.0	1.538022608209145	0.0
8	0.0	0.0	0.0	2.3651677178169983	0.0
9	0.0	0.0	0.0	2.814184807486903	0.0
10	0.0	0.0	0.0	3.5027306596816032	0.0
11	0.0	0.0	0.0	3.9621585880677483	0.0
12	0.0	0.0	0.0	4.409506764661004	0.0
13	0.0	0.0	0.0	4.625829688215249	0.0
14	0.0	0.0	0.0	4.72922282691623	0.0
15	0.0	0.0	0.0	4.829754971771528	0.0
16	0.0	0.0	0.0	5.038766466609023	0.0
17	0.0	0.0	0.0	5.267804918366309	0.0
18	0.0	0.0	0.0	5.647681323432176	0.0
19	0.0	0.0	0.0	5.789300518793551	0.0
20	0.0	0.0	0.0	5.946416764152383	0.0
21	0.0	0.0	0.0	6.121255276944204	0.0
22	0.0	0.0	0.0	6.309524566400488	0.0
23	0.0	0.0	0.0	6.513131961751691	0.0
24	0.0	0.0	0.0	6.6797053812115355	0.0
25	0.0	0.0	0.0	6.813774731702355	7.947205126900971E-5
26	0.0	0.0	0.0	6.9558707593713445	7.947205126900971E-5
27	0.0	0.0	0.0	7.122126290626112	7.947205126900971E-5
28	0.0	0.0	0.0	7.272487411627079	7.947205126900971E-5
29	0.0	0.0	0.0	7.428967880575759	7.947205126900971E-5
30	0.0	0.0	0.0	7.650933319770103	7.947205126900971E-5
31	0.0	0.0	0.0	7.818301459742638	7.947205126900971E-5
32	0.0	0.0	0.0	7.990596866893851	7.947205126900971E-5
33	0.0	0.0	0.0	8.153832460200396	7.947205126900971E-5
34	0.0	0.0	0.0	8.323425817608463	7.947205126900971E-5
35	0.0	0.0	0.0	8.509708305783022	7.947205126900971E-5
36	0.0	0.0	0.0	8.675010172422562	7.947205126900971E-5
37	0.0	0.0	0.0	8.851597070342303	7.947205126900971E-5
38	0.0	0.0	0.0	9.014832663648848	7.947205126900971E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	45	4.802132E-10	44.0	21
TAATTCG	30	2.5279096E-6	44.0	41
ACGAACC	20	7.8567665E-4	44.0	17
CGTTTTT	4260	0.0	41.3662	1
CACAACG	140	0.0	40.85714	12
CTAAGCG	40	4.1249587E-7	38.5	1
GGACCGA	690	0.0	38.260868	8
ACAACGA	150	0.0	38.13333	13
GACCGAT	655	0.0	37.954197	9
GCGATCT	175	0.0	37.714287	9
CTAGGGA	700	0.0	37.714287	4
ACGCACC	35	7.2907605E-6	37.714283	10
GCGATAC	70	0.0	37.714283	9
GACGGGT	135	0.0	37.48148	4
ACGGGTC	95	0.0	37.05263	5
TGTTACG	95	0.0	37.05263	1
GGCGATC	425	0.0	36.75294	8
TGGGCGA	785	0.0	36.713375	6
GGGCGAT	2745	0.0	36.706738	7
CGTAAGG	210	0.0	36.666668	2
>>END_MODULE