FastQCFastQC Report
Thu 26 May 2016
SRR1546516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546516_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2171712
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107480.4949090855509386No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28870.132936595644358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA207.858029E-444.00000428
CTATGCG600.044.01
CGTTTTT70250.041.024911
GCGTAAG1300.040.6153871
TCGCGAG557.8216544E-1140.01
CGGGTAT1700.037.529416
TCGATTG1350.037.481481
AGGGCGA25250.037.291096
TAGGGCG8750.037.2114265
GGCGATT13550.036.2066428
GGGCGAT48650.036.1767737
CGTTCGA1400.036.14285714
CGGTAGT8600.036.06976712
GCGCGAC4050.035.8518529
AAGGGAT37800.035.793655
ACGGGAT10650.035.737095
ACGCCGG9400.035.5744727
CGTTAGG2350.035.574472
AGGGATT39350.035.5019046
TAACGGG4800.035.2916643
Produced by FastQC (version 0.11.5)