##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546516_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2171712
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409482933280287	31.0	31.0	33.0	30.0	34.0
2	31.797977356113517	31.0	31.0	34.0	30.0	34.0
3	31.9340262428904	33.0	31.0	34.0	30.0	34.0
4	35.61231599770135	37.0	35.0	37.0	33.0	37.0
5	35.49992448354109	37.0	35.0	37.0	33.0	37.0
6	35.54739256402322	37.0	35.0	37.0	33.0	37.0
7	36.01663526287095	37.0	35.0	37.0	35.0	37.0
8	36.00799875858309	37.0	35.0	37.0	35.0	37.0
9	37.700591514897006	39.0	37.0	39.0	35.0	39.0
10	37.06002131037633	39.0	37.0	39.0	33.0	39.0
11	36.83820092166917	39.0	37.0	39.0	32.0	39.0
12	36.45191627619132	39.0	35.0	39.0	32.0	39.0
13	36.30926706671971	39.0	35.0	39.0	32.0	39.0
14	37.365018473904456	40.0	35.0	41.0	32.0	41.0
15	37.52252324433442	39.0	35.0	41.0	32.0	41.0
16	37.576543759025135	39.0	35.0	41.0	33.0	41.0
17	37.52524091592255	39.0	35.0	41.0	32.0	41.0
18	37.47407114755548	39.0	36.0	41.0	32.0	41.0
19	37.47596780788613	39.0	36.0	41.0	32.0	41.0
20	37.34323197551056	39.0	35.0	41.0	32.0	41.0
21	37.21948996920402	39.0	35.0	41.0	32.0	41.0
22	37.16658424321457	39.0	35.0	41.0	32.0	41.0
23	37.052576032181065	39.0	35.0	41.0	32.0	41.0
24	37.03605588586332	39.0	35.0	41.0	32.0	41.0
25	36.8593137579937	39.0	35.0	41.0	31.0	41.0
26	36.76787483791589	38.0	35.0	41.0	31.0	41.0
27	36.65773362213774	38.0	35.0	41.0	31.0	41.0
28	36.73182447764713	38.0	35.0	41.0	31.0	41.0
29	36.697540926237	38.0	35.0	41.0	31.0	41.0
30	36.677103133380484	38.0	35.0	41.0	31.0	41.0
31	36.55745467170601	38.0	35.0	41.0	31.0	41.0
32	36.37207419768367	38.0	35.0	41.0	30.0	41.0
33	36.31638863716736	39.0	35.0	41.0	30.0	41.0
34	36.23990381781746	39.0	35.0	41.0	30.0	41.0
35	36.08159323151504	38.0	35.0	41.0	30.0	41.0
36	35.97224355715675	38.0	35.0	41.0	29.0	41.0
37	35.92577054416055	38.0	35.0	41.0	29.0	41.0
38	35.913213170070435	38.0	35.0	41.0	29.0	41.0
39	35.78141807016768	38.0	35.0	40.0	29.0	41.0
40	35.70735069843515	38.0	35.0	40.0	28.0	41.0
41	35.690039471163765	38.0	35.0	40.0	28.0	41.0
42	35.53143188415407	38.0	35.0	40.0	27.0	41.0
43	35.567788454454366	38.0	35.0	40.0	28.0	41.0
44	35.343845316506055	38.0	34.0	40.0	27.0	41.0
45	35.259931335278345	38.0	34.0	40.0	27.0	41.0
46	35.26924150163558	38.0	34.0	40.0	27.0	41.0
47	35.18474963531076	38.0	34.0	40.0	27.0	41.0
48	35.146785116995254	37.0	34.0	40.0	27.0	41.0
49	35.1354608714231	37.0	34.0	40.0	27.0	41.0
50	34.594727109303626	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	8.0
13	5.0
14	16.0
15	40.0
16	119.0
17	326.0
18	708.0
19	1448.0
20	2634.0
21	4174.0
22	6304.0
23	9347.0
24	13448.0
25	19581.0
26	27050.0
27	33003.0
28	37394.0
29	42360.0
30	50036.0
31	61204.0
32	75938.0
33	96949.0
34	154497.0
35	236409.0
36	160475.0
37	204553.0
38	316379.0
39	617303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.976092594229804	23.65437958624348	27.73493907111072	13.634588748415997
2	32.21039438010197	25.70644726372558	28.817449090855508	13.265709265316946
3	29.581869050776533	25.871294167919135	30.871680959537912	13.675155821766422
4	25.37182646686117	28.50543718504111	30.752005790823095	15.370730557274628
5	22.952951404237762	32.880234579907466	29.0782111071818	15.088602908672973
6	20.662408275130403	41.9682259894498	26.560197668935842	10.809168066483954
7	87.27547667462352	3.903049759820824	6.797862700026523	2.023610865529131
8	87.2687078212949	3.2233095364394546	6.526049494592284	2.9819331476733564
9	80.75011787935048	6.035791117790941	9.529348274541007	3.684742728317567
10	38.14027826894174	33.69461512392067	15.001344561341467	13.163762045796126
11	31.924352768691243	25.062439218459907	27.846694220964842	15.166513791884007
12	30.051314354757906	23.427369743317712	28.78701227418737	17.73430362773701
13	22.958292812306606	29.784750464149944	30.48429073468305	16.772665988860403
14	18.87262215247694	33.16199385553886	30.050347375710963	17.915036616273245
15	17.328632894232754	25.39788885450741	40.33007139068164	16.943406860578197
16	19.783378274835705	24.10319600389002	37.622484012613086	18.490941708661186
17	19.843515162231455	23.089663822827337	30.671608390062772	26.39521262487844
18	22.313824300828102	23.936507234845138	33.46677644181181	20.282892022514957
19	25.521431939410018	26.47270908849792	28.260469159814928	19.745389812277132
20	26.673656543777447	25.79913911236849	27.13453717620016	20.392667167653904
21	23.653965166651933	28.201115064980993	28.97414574308196	19.17077402528512
22	22.773001208263345	25.439607093389917	28.23403839919842	23.553353299148323
23	19.462341231249816	30.182731411899923	27.829564877847524	22.52536247900274
24	19.535463265847405	26.324577107830137	33.754245498482305	20.385714127840153
25	19.86617009990275	27.65900819261486	31.365530972799338	21.109290734683054
26	19.10354595821177	29.960280184481185	29.1630289835853	21.773144873721744
27	19.96839359915127	28.61622535584829	29.638966861167592	21.77641418383285
28	18.734850661597854	27.844299796658124	33.963112972622525	19.457736569121504
29	19.77697779447735	25.68917978074441	32.65018565997701	21.883656764801227
30	21.43530081336752	27.004593610939203	30.62975201131642	20.930353564376862
31	24.74545427754693	26.269136975805264	27.269453776559693	21.715954970088113
32	26.042081086258218	26.73756925411841	27.709935755753985	19.510413903869388
33	23.791276191318186	26.160512996198392	27.714172044912033	22.33403876757139
34	20.419650487725814	26.352619500191555	30.823424100433204	22.404305911649427
35	20.18909505496125	26.3181305808505	30.99365845931689	22.499115904871363
36	24.601282306309493	25.68245697403707	28.458884050923878	21.257376668729556
37	21.102521881354434	28.759936860872898	29.607286785724813	20.530254472047858
38	21.606456104676862	29.116752129195767	27.541405121857775	21.735386644269592
39	20.763204329119148	28.551714039430642	29.237348230336252	21.44773340111396
40	23.18557893496007	26.166867429935458	28.67148130138803	21.97607233371644
41	19.426148586921286	25.274529956089943	29.901524695723925	25.397796761264846
42	22.993058011375357	26.66518396546135	27.13900369846462	23.20275432469867
43	21.69887167359208	26.214111263371937	28.97626388766098	23.110753175375002
44	21.267138552441576	28.107916703503967	29.406017004096306	21.21892773995815
45	19.601954586980224	29.42434355936699	28.422553266731498	22.551148586921286
46	22.10647636519023	28.56364011434297	28.16409358146937	21.165789938997438
47	21.427426841128106	26.980050761795304	29.166942946394364	22.425579450682225
48	22.560634190905606	25.123128665311057	29.383454159667583	22.932782984115757
49	21.792023988447824	24.586086921286064	30.502525196711165	23.119363893554947
50	19.968347552529984	28.10860740282321	29.73290196858515	22.19014307606165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1320.0
1	1726.5
2	2133.0
3	10389.5
4	18646.0
5	13350.0
6	8054.0
7	8409.5
8	8765.0
9	9069.0
10	9373.0
11	9348.0
12	9323.0
13	9063.5
14	8804.0
15	8534.5
16	8265.0
17	8060.0
18	7855.0
19	8194.0
20	8533.0
21	9651.0
22	10769.0
23	11692.0
24	12615.0
25	15027.5
26	17440.0
27	22030.5
28	26621.0
29	30407.5
30	34194.0
31	39001.0
32	43808.0
33	53288.5
34	62769.0
35	69443.0
36	76117.0
37	81671.5
38	87226.0
39	97765.5
40	108305.0
41	127364.5
42	146424.0
43	161614.5
44	176805.0
45	186645.5
46	196486.0
47	199878.5
48	203271.0
49	201002.5
50	198734.0
51	180284.0
52	161834.0
53	142866.0
54	123898.0
55	109238.0
56	94578.0
57	87336.5
58	80095.0
59	73030.5
60	65966.0
61	58412.5
62	50859.0
63	44035.5
64	37212.0
65	30070.5
66	22929.0
67	19215.0
68	15501.0
69	13097.0
70	10693.0
71	8346.5
72	6000.0
73	5028.0
74	4056.0
75	3535.5
76	3015.0
77	2169.5
78	1324.0
79	956.0
80	588.0
81	486.0
82	384.0
83	228.5
84	73.0
85	51.0
86	29.0
87	21.5
88	14.0
89	8.0
90	2.0
91	3.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2171712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.833204765982178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47720497963935	17.91886090762062
2	7.173990922434986	3.2761040744251084
3	2.4593208731933776	1.6846253124863542
4	1.35571211125915	1.238210089604087
5	0.8836788252360714	1.0088609781988898
6	0.7095901855825556	0.9721330804402671
7	0.5362309565843824	0.857070986348479
8	0.43034123470099706	0.786085562493877
9	0.3680136274397942	0.7562637460804229
>10	5.254137299959946	29.304416339953576
>50	2.0687227429833017	33.130077555775216
>100	0.28021757324942065	8.025839038903808
>500	0.0018248578307555182	0.2637080443834144
>1k	8.110479247802303E-4	0.28014350009902395
>5k	0.0	0.0
>10k+	2.0276198119505758E-4	0.49760078318696244
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10748	0.4949090855509386	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2887	0.132936595644358	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.604662128311673E-5	0.0	0.0	0.05171035570094009	0.0
2	4.604662128311673E-5	0.0	0.0	0.1786148439572098	0.0
3	4.604662128311673E-5	0.0	0.0	0.29704675389738605	0.0
4	4.604662128311673E-5	0.0	0.0	0.4742801992161023	0.0
5	4.604662128311673E-5	0.0	0.0	0.7930609583591195	0.0
6	4.604662128311673E-5	0.0	0.0	1.489562151887543	0.0
7	4.604662128311673E-5	0.0	0.0	1.9111189697344768	0.0
8	4.604662128311673E-5	0.0	0.0	3.2669156867945657	0.0
9	4.604662128311673E-5	0.0	0.0	4.032763091975363	0.0
10	4.604662128311673E-5	0.0	0.0	5.121903825184924	0.0
11	4.604662128311673E-5	0.0	0.0	5.669075825892199	0.0
12	4.604662128311673E-5	0.0	0.0	6.177108198508826	0.0
13	4.604662128311673E-5	0.0	0.0	6.459143753867917	0.0
14	4.604662128311673E-5	0.0	0.0	6.599309669053723	0.0
15	4.604662128311673E-5	0.0	0.0	6.712768543895323	0.0
16	4.604662128311673E-5	0.0	0.0	6.924767188282792	0.0
17	4.604662128311673E-5	0.0	0.0	7.1554607609112075	0.0
18	4.604662128311673E-5	0.0	0.0	7.538430510122889	0.0
19	4.604662128311673E-5	0.0	0.0	7.693929950195974	0.0
20	4.604662128311673E-5	0.0	0.0	7.889305764300238	0.0
21	4.604662128311673E-5	0.0	0.0	8.083714599357558	0.0
22	4.604662128311673E-5	0.0	0.0	8.2802415789939	0.0
23	4.604662128311673E-5	0.0	0.0	8.50393606518728	0.0
24	4.604662128311673E-5	0.0	0.0	8.67928159903339	0.0
25	4.604662128311673E-5	0.0	0.0	8.828472651990687	0.0
26	4.604662128311673E-5	0.0	0.0	8.975913933339227	0.0
27	4.604662128311673E-5	0.0	0.0	9.145503639524946	0.0
28	4.604662128311673E-5	0.0	0.0	9.304640762679398	0.0
29	4.604662128311673E-5	0.0	0.0	9.471974184422244	0.0
30	4.604662128311673E-5	0.0	0.0	9.724954321751687	0.0
31	4.604662128311673E-5	0.0	0.0	9.90729894203283	0.0
32	4.604662128311673E-5	0.0	0.0	10.091761706892996	0.0
33	4.604662128311673E-5	0.0	0.0	10.265357469130345	0.0
34	4.604662128311673E-5	0.0	0.0	10.441163469189284	0.0
35	4.604662128311673E-5	0.0	0.0	10.639256033949254	0.0
36	4.604662128311673E-5	0.0	0.0	10.81400296171868	0.0
37	4.604662128311673E-5	0.0	0.0	10.998189446851148	0.0
38	4.604662128311673E-5	0.0	0.0	11.194670379866206	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	20	7.858029E-4	44.000004	28
CTATGCG	60	0.0	44.0	1
CGTTTTT	7025	0.0	41.02491	1
GCGTAAG	130	0.0	40.615387	1
TCGCGAG	55	7.8216544E-11	40.0	1
CGGGTAT	170	0.0	37.52941	6
TCGATTG	135	0.0	37.48148	1
AGGGCGA	2525	0.0	37.29109	6
TAGGGCG	875	0.0	37.211426	5
GGCGATT	1355	0.0	36.206642	8
GGGCGAT	4865	0.0	36.176773	7
CGTTCGA	140	0.0	36.142857	14
CGGTAGT	860	0.0	36.069767	12
GCGCGAC	405	0.0	35.851852	9
AAGGGAT	3780	0.0	35.79365	5
ACGGGAT	1065	0.0	35.73709	5
ACGCCGG	940	0.0	35.57447	27
CGTTAGG	235	0.0	35.57447	2
AGGGATT	3935	0.0	35.501904	6
TAACGGG	480	0.0	35.291664	3
>>END_MODULE