FastQCFastQC Report
Thu 26 May 2016
SRR1546515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546515_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2139898
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122710.5734385470709351No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27010.12622096941069155No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA207.858005E-444.023
ACAATCG207.858005E-444.01
TCGACCA254.444131E-544.029
CGTTTTT73950.040.846521
CGTTAGG3200.039.8752
GTGTACG900.039.111111
TAATGCG900.039.111111
TTAGCCG1450.037.9310381
TCGATAT357.2931507E-637.71428733
TCGATAG1750.037.7142871
TAGGGCG8600.037.604655
AGGGCGA21100.037.2227486
GTTTGCG950.037.052631
AGGGATC23050.036.5553176
CGCATGG2350.036.5106352
GGGCGAT44300.036.4018067
AACGGGA8050.036.3478244
CGACGGT1400.036.14285728
TTAGGGA25950.035.6917154
AAGGGAT34550.035.5311135
Produced by FastQC (version 0.11.5)