FastQCFastQC Report
Thu 26 May 2016
SRR1546514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546514_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1387362
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63520.4578473390506588No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30950.22308525100154109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG700.044.02
CGTATAT207.857047E-444.035
CTATCGA207.857047E-444.044
GCGAAGT254.443319E-544.09
GCTATCG207.857047E-444.043
TTCACGA207.857047E-444.023
TCCGTGC254.443319E-544.020
CGTAACA351.4463694E-744.035
CGGTCTA850.044.031
CGGTCGT207.857047E-444.014
GCGATAA1300.043.9999969
CGCATGG1850.041.6216242
CGAGGGT1600.041.254
CGTTTTT43700.040.8283771
AGGGCGA13350.039.2209746
AGGGTAC6100.038.5901646
TCGTTAG404.1253588E-738.51
GGCGATA5500.038.48
CTTCGCA357.291299E-637.71428720
TTACGAG1000.037.3999981
Produced by FastQC (version 0.11.5)