##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546514_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1387362
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422406697026442	31.0	31.0	33.0	30.0	34.0
2	31.80298941444266	31.0	31.0	34.0	30.0	34.0
3	31.94117901456145	33.0	31.0	34.0	30.0	34.0
4	35.6074405958935	37.0	35.0	37.0	33.0	37.0
5	35.484724967239984	37.0	35.0	37.0	33.0	37.0
6	35.53073458837708	37.0	35.0	37.0	33.0	37.0
7	36.003882187922116	37.0	35.0	37.0	35.0	37.0
8	36.00020110108249	37.0	35.0	37.0	35.0	37.0
9	37.70166690452816	39.0	37.0	39.0	35.0	39.0
10	37.06691836737636	39.0	37.0	39.0	33.0	39.0
11	36.822114920258734	39.0	37.0	39.0	32.0	39.0
12	36.18678758680143	38.0	35.0	39.0	32.0	39.0
13	35.8895839730366	38.0	35.0	39.0	31.0	39.0
14	36.907695323931314	39.0	35.0	41.0	31.0	41.0
15	37.17221028109462	39.0	35.0	41.0	32.0	41.0
16	37.280062449454434	39.0	35.0	41.0	32.0	41.0
17	37.23480605638615	39.0	35.0	41.0	32.0	41.0
18	37.19328985513514	39.0	35.0	41.0	32.0	41.0
19	37.18030405907038	39.0	35.0	41.0	32.0	41.0
20	36.99461712227955	38.0	35.0	41.0	32.0	41.0
21	36.87910797614465	38.0	35.0	41.0	32.0	41.0
22	36.8028877827128	38.0	35.0	41.0	32.0	41.0
23	36.72515104204959	38.0	35.0	41.0	31.0	41.0
24	36.672843136830906	38.0	35.0	41.0	31.0	41.0
25	36.51867573135202	38.0	35.0	40.0	31.0	41.0
26	36.39170382351542	38.0	35.0	40.0	31.0	41.0
27	36.279516809599805	38.0	35.0	40.0	31.0	41.0
28	36.38345795834108	38.0	35.0	40.0	31.0	41.0
29	36.33557355614468	38.0	35.0	40.0	31.0	41.0
30	36.33899515771659	38.0	35.0	40.0	31.0	41.0
31	36.202138302764524	38.0	35.0	40.0	30.0	41.0
32	36.001522313570646	38.0	35.0	40.0	30.0	41.0
33	35.91211306061432	38.0	35.0	40.0	30.0	41.0
34	35.85013356283363	38.0	35.0	40.0	30.0	41.0
35	35.657715145722605	38.0	35.0	40.0	29.0	41.0
36	35.51666616211198	38.0	35.0	40.0	28.0	41.0
37	35.42126424105605	38.0	34.0	40.0	27.0	41.0
38	35.410393970715646	38.0	34.0	40.0	28.0	41.0
39	35.334454886323826	38.0	34.0	40.0	27.0	41.0
40	35.24432556174956	37.0	34.0	40.0	27.0	41.0
41	35.27796350195551	37.0	34.0	40.0	27.0	41.0
42	35.10687765702102	37.0	34.0	40.0	27.0	41.0
43	35.11720084592197	37.0	34.0	40.0	27.0	41.0
44	34.7944422580408	37.0	34.0	40.0	26.0	41.0
45	34.73396417085087	37.0	34.0	40.0	26.0	41.0
46	34.76015416308073	36.0	34.0	40.0	26.0	41.0
47	34.66621256744815	36.0	34.0	40.0	26.0	41.0
48	34.679868700454534	36.0	34.0	40.0	26.0	41.0
49	34.69333310267976	36.0	34.0	40.0	26.0	41.0
50	34.121451358765775	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	12.0
14	14.0
15	34.0
16	93.0
17	196.0
18	565.0
19	1079.0
20	1896.0
21	3182.0
22	4721.0
23	7088.0
24	9933.0
25	13849.0
26	18694.0
27	22503.0
28	25686.0
29	28585.0
30	33903.0
31	41860.0
32	51655.0
33	67123.0
34	112647.0
35	189846.0
36	98107.0
37	121825.0
38	183425.0
39	348835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.405079568274175	24.139049505464328	27.496788869811915	13.959082056449578
2	31.168865804310624	25.618836323901046	29.687709480294256	13.524588391494072
3	29.609575583012944	26.606033609108508	30.159468112864555	13.624922695013991
4	25.165746214758656	28.93448141148453	30.614864757720046	15.284907616036767
5	23.14839241668721	32.81364200547514	29.448478479301006	14.589487098536647
6	20.339176076611583	41.37852990063156	27.148934452579788	11.13335957017707
7	86.23495526041509	4.439937089238425	7.164460321098604	2.160647329247882
8	86.2594622023668	3.9482125069015876	6.514882200896377	3.2774430898352414
9	80.32640363510029	5.836976938967624	9.568230930355595	4.268388495576497
10	44.60155316348581	27.18973130300527	14.952333997903935	13.256381535604984
11	38.52599393669424	23.108100120948965	24.37143297855931	13.994472963797481
12	35.029934508801595	21.738594541294916	26.207363326947114	17.02410762295637
13	22.730621135651692	34.19086006391987	27.25128697484867	15.82723182557977
14	17.345508958728868	38.15456960764386	28.406356812425308	16.093564621201963
15	15.553042392684821	23.83963233820733	44.61229297039994	15.995032298707907
16	17.121054202147672	21.102927714612335	42.49676724603961	19.279250837200383
17	18.23813827969917	21.186323396489165	29.6685364021791	30.907001921632567
18	21.376396355096936	23.064852576328313	34.03423187315207	21.52451919542268
19	27.014939143496797	26.115894770074426	26.59810489259472	20.271061193834054
20	28.23675435827131	24.73528898730108	25.498175674409417	21.52978098001819
21	22.35270967490821	29.02479670050066	28.290165075877816	20.332328548713313
22	22.402876826668166	25.199911775008978	26.94790544933478	25.449305948988076
23	19.849397633782676	30.378300688645073	25.411968902132248	24.360332775440007
24	19.873688338011274	26.08035970424446	34.92988852224582	19.116063435498447
25	19.217478927633884	25.818423742325365	33.415287430389476	21.548809899651282
26	17.48145040731979	32.21394272006874	28.724802899315392	21.579803973296084
27	19.51574282703433	30.571617213099394	28.460055846995953	21.452584112870323
28	17.95738963587009	27.686933907660727	35.706037789704496	18.649638666764695
29	18.529266334237207	25.08314340453321	34.615695110576766	21.771895150652824
30	20.961940719149002	28.226591185285454	30.52937877785322	20.28208931771232
31	26.70283602981774	26.718116828917037	24.706168973923173	21.87287816734205
32	28.77691619058328	25.936273301416644	25.70734963189132	19.579460876108758
33	25.859364751232917	27.513439174490866	25.282514585234424	21.344681489041793
34	22.012423577984695	27.563390088527722	27.897837766927445	22.526348566560134
35	20.682345343176475	26.630468471819178	29.241178582086004	23.446007602918346
36	27.351765436850656	25.971592129523515	26.078918119423772	20.59772431420206
37	21.402705278074503	30.18411921329833	27.352702466984102	21.06047304164306
38	20.975924091909683	31.074009523109325	25.64175752254999	22.308308862431005
39	20.204099578913073	29.38713904518071	27.28538045585795	23.123380920048263
40	22.679228636794146	25.594257302708307	27.769392559404103	23.957121501093443
41	18.065508497421725	24.71950363351454	28.22486128350063	28.990126585563104
42	22.664236154658987	26.030408790207606	25.75852589302576	25.546829162107652
43	22.143175321221136	26.499644649341704	27.42218685534129	23.934993174095872
44	21.086493647656486	29.447757686890657	28.09583944204901	21.36990922340384
45	18.99223129940131	31.740886661159813	26.259188301250862	23.007693738188014
46	23.081502881007264	29.66803184749186	26.925128409167904	20.325336862332975
47	21.729945032370786	27.074116200386054	27.84240883057198	23.35352993667118
48	23.317922791600175	23.670246121776437	29.13796110892471	23.873869977698682
49	21.968166923989557	22.744748666894438	30.61407188606867	24.673012523047337
50	20.062824266485606	28.604214329064803	28.327285885010546	23.005675519439052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1418.0
1	1610.0
2	1802.0
3	6266.0
4	10730.0
5	7735.5
6	4741.0
7	5016.0
8	5291.0
9	5700.0
10	6109.0
11	6237.5
12	6366.0
13	6191.5
14	6017.0
15	5713.5
16	5410.0
17	5336.0
18	5262.0
19	5585.0
20	5908.0
21	6011.0
22	6114.0
23	6573.5
24	7033.0
25	8001.5
26	8970.0
27	11182.5
28	13395.0
29	14978.0
30	16561.0
31	20138.5
32	23716.0
33	27573.0
34	31430.0
35	33298.0
36	35166.0
37	39248.5
38	43331.0
39	53459.0
40	63587.0
41	80801.5
42	98016.0
43	111670.5
44	125325.0
45	132598.0
46	139871.0
47	138090.0
48	136309.0
49	133530.5
50	130752.0
51	117175.5
52	103599.0
53	91190.5
54	78782.0
55	69981.5
56	61181.0
57	56238.0
58	51295.0
59	48097.0
60	44899.0
61	39232.0
62	33565.0
63	29387.0
64	25209.0
65	21466.0
66	17723.0
67	14614.5
68	11506.0
69	9798.5
70	8091.0
71	7028.0
72	5965.0
73	4567.0
74	3169.0
75	2444.0
76	1719.0
77	1371.5
78	1024.0
79	820.5
80	617.0
81	415.5
82	214.0
83	142.0
84	70.0
85	46.0
86	22.0
87	35.0
88	48.0
89	32.5
90	17.0
91	12.5
92	8.0
93	8.5
94	9.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1387362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.709496211355255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97867463897089	18.16275408702924
2	6.7669861152877635	3.0734969109484216
3	2.299575970628881	1.5666663537816046
4	1.2141354399463273	1.1028961669413306
5	0.8221131543530519	0.9334887782042975
6	0.5964398320313411	0.8126908863490262
7	0.48962394048126867	0.7783379114943741
8	0.3991896996577214	0.7252317577591252
9	0.33554848940111354	0.6858123433902509
>10	4.585228019371906	25.889532086931165
>50	2.1478568047057998	34.756565429700274
>100	0.35951927316520305	10.169517535128868
>500	0.004150755374016426	0.5761556793062473
>1k	6.38577749848681E-4	0.3062775722866334
>5k	3.192888749243405E-4	0.46057650074921763
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6352	0.4578473390506588	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3095	0.22308525100154109	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05903289840719293	0.0
2	0.0	0.0	0.0	0.22640089608912453	0.0
3	0.0	0.0	0.0	0.3582338279410853	0.0
4	0.0	0.0	0.0	0.5999155231295077	0.0
5	0.0	0.0	0.0	1.0305169090691542	0.0
6	0.0	0.0	0.0	1.7630582357020013	0.0
7	0.0	0.0	0.0	2.2088683414999113	0.0
8	0.0	0.0	0.0	3.6314242425552954	0.0
9	0.0	0.0	0.0	4.410744996619483	0.0
10	0.0	0.0	0.0	5.532442145597184	0.0
11	0.0	0.0	0.0	6.104751319410507	0.0
12	0.0	0.0	0.0	6.695368620446574	0.0
13	0.0	0.0	0.0	6.949736262057055	0.0
14	0.0	0.0	0.0	7.059440866911448	0.0
15	0.0	0.0	0.0	7.176065078905145	0.0
16	0.0	0.0	0.0	7.4080160765539205	0.0
17	0.0	0.0	0.0	7.654455001650614	0.0
18	0.0	0.0	0.0	8.076695195630268	0.0
19	0.0	0.0	0.0	8.234909129700828	0.0
20	0.0	0.0	0.0	8.431252982278599	0.0
21	0.0	0.0	0.0	8.624281189768784	0.0
22	0.0	0.0	0.0	8.81911137828483	0.0
23	0.0	0.0	0.0	9.033042565675	0.0
24	0.0	0.0	0.0	9.1982481861259	0.0
25	0.0	0.0	0.0	9.32517972958752	0.0
26	0.0	0.0	0.0	9.459102959429478	0.0
27	0.0	0.0	0.0	9.60189193591867	0.0
28	7.207924103442361E-5	0.0	0.0	9.73451773942201	0.0
29	7.207924103442361E-5	0.0	0.0	9.885523749389128	0.0
30	7.207924103442361E-5	0.0	0.0	10.10356345351826	0.0
31	7.207924103442361E-5	0.0	0.0	10.275904918831566	0.0
32	7.207924103442361E-5	0.0	0.0	10.435560437722815	0.0
33	7.207924103442361E-5	0.0	0.0	10.586782685413036	0.0
34	7.207924103442361E-5	0.0	0.0	10.748384343812214	0.0
35	7.207924103442361E-5	0.0	0.0	10.933123438583442	0.0
36	1.4415848206884721E-4	0.0	0.0	11.088598361494693	0.0
37	1.4415848206884721E-4	0.0	0.0	11.249767544447664	0.0
38	1.4415848206884721E-4	0.0	0.0	11.416126432755114	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	70	0.0	44.0	2
CGTATAT	20	7.857047E-4	44.0	35
CTATCGA	20	7.857047E-4	44.0	44
GCGAAGT	25	4.443319E-5	44.0	9
GCTATCG	20	7.857047E-4	44.0	43
TTCACGA	20	7.857047E-4	44.0	23
TCCGTGC	25	4.443319E-5	44.0	20
CGTAACA	35	1.4463694E-7	44.0	35
CGGTCTA	85	0.0	44.0	31
CGGTCGT	20	7.857047E-4	44.0	14
GCGATAA	130	0.0	43.999996	9
CGCATGG	185	0.0	41.621624	2
CGAGGGT	160	0.0	41.25	4
CGTTTTT	4370	0.0	40.828377	1
AGGGCGA	1335	0.0	39.220974	6
AGGGTAC	610	0.0	38.590164	6
TCGTTAG	40	4.1253588E-7	38.5	1
GGCGATA	550	0.0	38.4	8
CTTCGCA	35	7.291299E-6	37.714287	20
TTACGAG	100	0.0	37.399998	1
>>END_MODULE