FastQCFastQC Report
Thu 26 May 2016
SRR1546513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546513_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1881609
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166600.8854124315944493No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21900.11638974941127515No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC20800.11054368893856267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89250.042.0280081
TACCGGT750.041.06666640
TACGGGA5700.038.210534
TTTAGCG1500.038.133331
ACGGGAT4150.037.6385545
GCCCTAC7250.037.62758626
AGGGTAC9950.037.5879366
CTAATCC7950.037.3584911
CATATGC14200.037.33802833
GTCTCAA14150.037.31448742
ATATCGT301.3014107E-436.66666421
TAGGGCA16150.036.3715175
GTTGATC15200.036.1842116
GGTACCT16600.036.180728
TACGCGG1400.036.1428572
TATCACG553.426976E-936.01
TAGCATA14850.036.030
CCCTAGC7900.035.92405321
CGCATCG1900.035.89473721
GGGTACC17750.035.8197177
Produced by FastQC (version 0.11.5)