FastQCFastQC Report
Thu 26 May 2016
SRR1546512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546512_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1712459
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183991.0744198839213084No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC18960.11071797923337143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG750.044.01
CGTTTTT102150.042.0401341
TACGGGT1150.040.173914
CGGGTAT1050.039.8095256
TCGATAG501.3478711E-939.61
TATACGG900.039.111112
TTACGGG5100.037.9607853
GCGATAT2000.037.3999989
TAGCCGT1650.037.33333244
CGTAAGG2950.037.2881362
TATTACG651.0913936E-1137.230771
CGGTCTA1300.037.2307731
GTTTTTT120800.037.0248342
TAGACGG2200.037.02
GGGCGAT36150.036.5753827
GCGCGAC12450.036.22499
AGGGCGA19050.035.8005266
TGTTACG800.035.751
TAACGGG3400.035.5882383
TAGCATA14400.035.44444730
Produced by FastQC (version 0.11.5)