##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546512_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1712459
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330807336117243	31.0	31.0	33.0	30.0	34.0
2	31.734105166897425	31.0	31.0	34.0	30.0	34.0
3	31.860709657866263	31.0	31.0	34.0	30.0	34.0
4	35.56437088420803	37.0	35.0	37.0	33.0	37.0
5	35.45802790023002	37.0	35.0	37.0	33.0	37.0
6	35.486065359813	37.0	35.0	37.0	33.0	37.0
7	35.94286344957748	37.0	35.0	37.0	35.0	37.0
8	35.92587034200527	37.0	35.0	37.0	35.0	37.0
9	37.6017948458912	39.0	37.0	39.0	35.0	39.0
10	36.99950305379574	39.0	37.0	39.0	33.0	39.0
11	36.77989078862618	39.0	37.0	39.0	32.0	39.0
12	36.44448071457477	39.0	35.0	39.0	32.0	39.0
13	36.36255057785325	39.0	35.0	39.0	32.0	39.0
14	37.426292833872225	40.0	36.0	41.0	32.0	41.0
15	37.546191178883696	40.0	36.0	41.0	32.0	41.0
16	37.571458937119075	40.0	36.0	41.0	32.0	41.0
17	37.47348812438721	39.0	35.0	41.0	32.0	41.0
18	37.3817241755861	39.0	36.0	41.0	32.0	41.0
19	37.31567295917742	39.0	36.0	41.0	32.0	41.0
20	37.12894732078257	39.0	35.0	41.0	32.0	41.0
21	37.027386349103836	39.0	35.0	41.0	31.0	41.0
22	36.94779145077342	39.0	35.0	41.0	31.0	41.0
23	36.84693764931014	38.0	35.0	41.0	31.0	41.0
24	36.77178373321639	38.0	35.0	41.0	31.0	41.0
25	36.57856392474214	38.0	35.0	41.0	31.0	41.0
26	36.46847895336472	38.0	35.0	40.0	31.0	41.0
27	36.38096211354549	38.0	35.0	40.0	30.0	41.0
28	36.38577974713555	38.0	35.0	40.0	30.0	41.0
29	36.27713189045694	38.0	35.0	40.0	30.0	41.0
30	36.213234302251905	38.0	35.0	40.0	30.0	41.0
31	36.10377825104134	38.0	35.0	40.0	30.0	41.0
32	35.847225539414374	38.0	35.0	40.0	29.0	41.0
33	35.717688423489264	38.0	35.0	40.0	28.0	41.0
34	35.54466355106896	38.0	35.0	40.0	27.0	41.0
35	35.32336540612067	38.0	34.0	40.0	26.0	41.0
36	35.166389969044516	38.0	34.0	40.0	25.0	41.0
37	35.12699515725632	38.0	34.0	40.0	25.0	41.0
38	35.05154517568012	38.0	34.0	40.0	25.0	41.0
39	34.92061999732548	38.0	34.0	40.0	24.0	41.0
40	34.77241615711675	37.0	34.0	40.0	24.0	41.0
41	34.72555430524176	37.0	34.0	40.0	24.0	41.0
42	34.50450142163987	37.0	33.0	40.0	23.0	41.0
43	34.51090741442569	37.0	33.0	40.0	23.0	41.0
44	34.232054022899234	36.0	33.0	40.0	23.0	41.0
45	34.11879934059735	36.0	33.0	40.0	23.0	41.0
46	34.100118601379656	36.0	33.0	40.0	23.0	41.0
47	33.97552233367339	36.0	33.0	40.0	23.0	41.0
48	33.91439853450506	36.0	33.0	40.0	23.0	41.0
49	33.88509097152107	35.0	33.0	40.0	23.0	41.0
50	33.37709632756171	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	6.0
12	10.0
13	10.0
14	21.0
15	71.0
16	172.0
17	354.0
18	862.0
19	1586.0
20	2775.0
21	4452.0
22	6586.0
23	9727.0
24	13651.0
25	19875.0
26	27727.0
27	34664.0
28	37964.0
29	40245.0
30	44897.0
31	52736.0
32	64064.0
33	81125.0
34	124408.0
35	171000.0
36	144357.0
37	182983.0
38	255659.0
39	390470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.640303797054415	22.905599491725056	25.79600445908486	16.658092252135674
2	35.79151384062334	24.44963645844952	26.20202877849922	13.556820922427923
3	29.959666187628436	24.738110518266424	31.276894804488748	14.025328489616392
4	26.187196306597706	26.897811860021175	31.1813596705089	15.73363216287222
5	22.890007877560866	31.04915212568593	30.188576777604602	15.872263219148605
6	20.680728706497497	39.42021385621495	28.629882525654626	11.26917491163292
7	83.12794642090701	4.298555469065245	10.008239613327968	2.5652584966997747
8	82.55432684811724	3.8659027748985526	9.989903407906409	3.5898669690778
9	76.68160230405516	5.451400588276858	13.174388408715188	4.692608698952792
10	40.85651101719808	24.451913885237545	19.06504038928815	15.626534708276226
11	32.18354424835865	25.18273430195993	25.45993801895403	17.173783430727394
12	28.033430289425908	21.091599857281253	30.908827598208187	19.966142255084648
13	24.176520430562135	24.676736786106996	32.006372123361785	19.140370659969086
14	20.860470236075727	27.79324935662693	32.3412122567606	19.005068150536744
15	19.908914607590606	22.169348288046603	37.96324466746357	19.95849243689922
16	22.713186125916007	20.71091921032854	35.292874165162495	21.283020498592958
17	22.05693683761188	21.030109334004493	31.521280217511777	25.39167361087185
18	24.594632630620644	21.38258492612086	32.192945933304095	21.829836509954397
19	27.874886347643944	24.477315953257857	27.12158364083461	20.526214058263584
20	28.357525640029923	23.90562343390411	26.4235815280833	21.31326939798267
21	25.959512023353554	25.3119052777322	28.434549381912213	20.294033317002043
22	24.59031135927926	22.722821393096126	28.124468965388367	24.56239828223625
23	23.289375103287142	25.741463007289518	27.270083546525786	23.699078342897554
24	23.251301199036007	23.65031805141028	31.310238668487827	21.788142081065885
25	24.20700291218651	23.747955425502155	29.26902191526921	22.77601974704212
26	21.669891074764415	25.52078619108545	28.65341593579759	24.155906798352543
27	22.987294878300734	23.360092124833354	28.957072840868015	24.695540155997893
28	22.624658458976242	25.086732003510743	30.38910712606842	21.899502411444598
29	23.005047128135622	23.84722787523672	30.074238273733854	23.073486722893804
30	24.20414152981181	23.317521762564827	29.704769574045276	22.773567133578087
31	26.165531554332105	23.97838430000368	26.09271229267387	23.763371852990346
32	26.521335693292507	24.065043309066088	26.41937704785925	22.994243949782156
33	25.39260793981053	23.468649468396034	26.599235368554808	24.53950722323863
34	22.501677412422723	26.46568472588249	28.227245148642975	22.805392713051816
35	22.980112224584648	25.179639337350558	28.744337820642713	23.09591061742208
36	25.705199365357068	26.160451140728043	26.236248575878314	21.89810091803658
37	23.506840163764505	27.494731260719234	26.291549169936328	22.70687940557993
38	25.10442585778696	27.012559132802593	25.68773909331552	22.195275916094925
39	23.866323222920958	25.514654657425375	25.443412075851157	25.17561004380251
40	25.376432370059664	23.29480589024321	28.007911430288257	23.320850309408865
41	22.465413770490272	23.76938659553309	27.674005625828123	26.091194008148516
42	24.375649285617936	25.550217552653816	25.372461472070277	24.70167168965797
43	23.42602071056884	24.01348003076278	27.76504430179058	24.795454956877798
44	23.709998312368356	25.041066676632838	27.89503281538419	23.35390219561461
45	22.38354319723859	25.311438113262856	28.10426410208945	24.2007545874091
46	24.140023206395014	24.783892636261655	27.544542672262516	23.531541485080812
47	22.727609828906854	25.969556059444344	27.816432393417884	23.48640171823092
48	23.81908121595904	24.441577871353417	28.079504385214477	23.659836527473065
49	24.136636263992305	23.158919425224195	28.214047752384147	24.490396558399354
50	22.38984991757467	24.61915876526095	29.106390284380527	23.884601032783852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1370.0
1	1829.5
2	2289.0
3	15643.5
4	28998.0
5	19723.0
6	10448.0
7	10435.0
8	10422.0
9	10762.5
10	11103.0
11	10987.5
12	10872.0
13	10411.0
14	9950.0
15	9490.0
16	9030.0
17	8769.0
18	8508.0
19	7939.5
20	7371.0
21	7546.5
22	7722.0
23	7782.0
24	7842.0
25	8478.0
26	9114.0
27	10543.5
28	11973.0
29	14018.5
30	16064.0
31	18574.5
32	21085.0
33	23754.0
34	26423.0
35	30549.0
36	34675.0
37	38551.5
38	42428.0
39	48184.5
40	53941.0
41	63395.0
42	72849.0
43	83390.0
44	93931.0
45	104767.5
46	115604.0
47	125114.5
48	134625.0
49	139463.5
50	144302.0
51	131970.5
52	119639.0
53	110473.5
54	101308.0
55	98332.5
56	95357.0
57	95588.0
58	95819.0
59	92218.0
60	88617.0
61	83894.5
62	79172.0
63	72970.0
64	66768.0
65	58058.0
66	49348.0
67	43015.0
68	36682.0
69	32257.0
70	27832.0
71	24121.0
72	20410.0
73	16971.5
74	13533.0
75	10741.0
76	7949.0
77	5791.5
78	3634.0
79	2707.5
80	1781.0
81	1367.5
82	954.0
83	680.0
84	406.0
85	295.0
86	184.0
87	131.5
88	79.0
89	50.5
90	22.0
91	19.5
92	17.0
93	11.0
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1712459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.84313015869871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7658926442027	21.853255031267313
2	6.497863278103881	3.748374325675579
3	2.7035705011611073	2.3393830757462437
4	1.7290288741205746	1.994824194576321
5	1.2709553136096945	1.832916476816708
6	1.0197474854486834	1.7647625671081257
7	0.8715159853108204	1.7596074299794582
8	0.710331030377048	1.6390536291942215
9	0.6505808352464122	1.688830893879047
>10	8.489585628513721	52.75872440097126
>50	0.24896477042290233	4.468846301482229
>100	0.0374821081937767	2.0378150741097096
>500	0.0036667188725723974	0.7929623512834487
>1k	6.111198120953995E-4	0.23960436192456314
>5k	0.0	0.0
>10k+	2.0370660403179982E-4	1.0810398859857864
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18399	1.0744198839213084	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1896	0.11071797923337143	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.060848172131420374	0.0
2	0.0	0.0	0.0	0.21209266907996047	0.0
3	0.0	0.0	0.0	0.3765345622873307	0.0
4	0.0	0.0	0.0	0.5569184430108983	0.0
5	0.0	0.0	0.0	0.9543002197424872	0.0
6	0.0	0.0	0.0	1.7865537218701295	0.0
7	0.0	0.0	0.0	2.2613680093946775	0.0
8	0.0	0.0	0.0	3.4552068107907985	0.0
9	0.0	0.0	0.0	4.126463757672447	0.0
10	0.0	0.0	0.0	5.013258711595431	0.0
11	0.0	0.0	0.0	5.5027302843454935	0.0
12	0.0	0.0	0.0	5.941397721054927	0.0
13	0.0	0.0	0.0	6.208966170868909	0.0
14	0.0	0.0	0.0	6.3459037559439375	0.0
15	0.0	0.0	0.0	6.458198415261329	0.0
16	0.0	0.0	0.0	6.651896483361061	0.0
17	0.0	0.0	0.0	6.83963820447672	0.0
18	0.0	0.0	0.0	7.1838800228209845	0.0
19	0.0	0.0	0.0	7.310656780687888	0.0
20	5.839555866738999E-5	0.0	0.0	7.472412478196558	0.0
21	5.839555866738999E-5	0.0	0.0	7.6203284283010575	0.0
22	5.839555866738999E-5	0.0	0.0	7.777412481116336	0.0
23	5.839555866738999E-5	0.0	0.0	7.962292819857293	0.0
24	5.839555866738999E-5	5.839555866738999E-5	0.0	8.107288992028423	0.0
25	5.839555866738999E-5	5.839555866738999E-5	0.0	8.232080300900634	0.0
26	5.839555866738999E-5	5.839555866738999E-5	0.0	8.357981125387527	0.0
27	5.839555866738999E-5	5.839555866738999E-5	0.0	8.50134222191597	0.0
28	5.839555866738999E-5	5.839555866738999E-5	0.0	8.637228686934986	0.0
29	5.839555866738999E-5	5.839555866738999E-5	0.0	8.780472992346095	0.0
30	1.1679111733477998E-4	5.839555866738999E-5	0.0	8.973119940389815	0.0
31	1.1679111733477998E-4	5.839555866738999E-5	0.0	9.127225819713056	0.0
32	1.1679111733477998E-4	5.839555866738999E-5	0.0	9.317011385382074	0.0
33	1.1679111733477998E-4	5.839555866738999E-5	0.0	9.486767274428177	0.0
34	1.7518667600216999E-4	5.839555866738999E-5	0.0	9.642216251600768	0.0
35	1.7518667600216999E-4	5.839555866738999E-5	0.0	9.816585389781595	0.0
36	2.3358223466955996E-4	5.839555866738999E-5	0.0	9.980326536284956	0.0
37	2.3358223466955996E-4	5.839555866738999E-5	0.0	10.14179025600029	0.0
38	2.3358223466955996E-4	5.839555866738999E-5	0.0	10.341619857760099	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	75	0.0	44.0	1
CGTTTTT	10215	0.0	42.040134	1
TACGGGT	115	0.0	40.17391	4
CGGGTAT	105	0.0	39.809525	6
TCGATAG	50	1.3478711E-9	39.6	1
TATACGG	90	0.0	39.11111	2
TTACGGG	510	0.0	37.960785	3
GCGATAT	200	0.0	37.399998	9
TAGCCGT	165	0.0	37.333332	44
CGTAAGG	295	0.0	37.288136	2
TATTACG	65	1.0913936E-11	37.23077	1
CGGTCTA	130	0.0	37.23077	31
GTTTTTT	12080	0.0	37.024834	2
TAGACGG	220	0.0	37.0	2
GGGCGAT	3615	0.0	36.575382	7
GCGCGAC	1245	0.0	36.2249	9
AGGGCGA	1905	0.0	35.800526	6
TGTTACG	80	0.0	35.75	1
TAACGGG	340	0.0	35.588238	3
TAGCATA	1440	0.0	35.444447	30
>>END_MODULE