FastQCFastQC Report
Thu 26 May 2016
SRR1546511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546511_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1606357
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101510.6319267759283895No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA24070.1498421583745083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG408.3164196E-944.01
GCGTAAG1000.041.81
CGTAAGG1700.041.4117662
CGTTTTT58600.040.92151
AGTACGG1650.038.6666682
GTATACG750.038.133331
GCGCGAC6700.037.7611969
TAGGGAC10100.037.683175
TACGTAG950.037.052631
TATACGG1850.036.8648642
ATAGGGA15900.036.805034
TATGGGC6900.036.6666684
TATAGCG601.9826984E-1036.6666681
TAGTCGG601.9826984E-1036.6666682
GTAGGGC7450.036.3221474
TAGGGCG7450.036.3221475
GCGGGAT4800.036.2083365
ACGTAAG553.426976E-936.01
TTAACGG1350.035.8518522
TACGGGA5050.035.7227754
Produced by FastQC (version 0.11.5)