FastQCFastQC Report
Thu 26 May 2016
SRR1546510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546510_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences677011
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58210.8598087771099732No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8690.12835832800353317No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAT207.8541867E-444.023
ACCCGTA207.8541867E-444.027
AAGTGCG207.8541867E-444.01
CGTTTTT35000.042.617141
TAGACGG700.040.8571432
CGTTCGA603.6379788E-1240.33333214
ACGGGCG557.8216544E-1140.0000045
TATACGG501.3460522E-939.62
TAGCACG452.3484972E-839.111111
TACGAAT452.3484972E-839.1111112
ACGGGAT4000.039.055
AGGGCGA9150.038.9508176
CGAACGG404.1211933E-738.52
TTATGCG800.038.51
TTCACGG404.1211933E-738.52
GGACCGT404.1211933E-738.58
CGTTAGG1150.038.2608682
TACGGGT750.038.133334
TAGGGAC6950.037.9856155
ATAGGGC3550.037.802824
Produced by FastQC (version 0.11.5)