##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546510_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	677011
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.594762862050985	31.0	31.0	34.0	30.0	34.0
2	32.002100409003695	33.0	31.0	34.0	30.0	34.0
3	32.1129036308125	33.0	31.0	34.0	30.0	34.0
4	35.775337476052826	37.0	35.0	37.0	35.0	37.0
5	35.68201550639502	37.0	35.0	37.0	33.0	37.0
6	35.72387597838144	37.0	35.0	37.0	33.0	37.0
7	36.129797004775405	37.0	35.0	37.0	35.0	37.0
8	36.132175104983524	37.0	35.0	37.0	35.0	37.0
9	37.8521456815325	39.0	38.0	39.0	35.0	39.0
10	37.2613044692036	39.0	37.0	39.0	34.0	39.0
11	37.078156780318196	39.0	37.0	39.0	33.0	39.0
12	36.75524031367289	39.0	35.0	39.0	33.0	39.0
13	36.665516513025636	39.0	35.0	39.0	33.0	39.0
14	37.78764746806182	40.0	36.0	41.0	33.0	41.0
15	37.902156685785016	40.0	36.0	41.0	33.0	41.0
16	37.94153270774035	40.0	36.0	41.0	33.0	41.0
17	37.880046262172996	40.0	36.0	41.0	33.0	41.0
18	37.793507047891396	39.0	36.0	41.0	33.0	41.0
19	37.74700706487782	39.0	36.0	41.0	33.0	41.0
20	37.593829346938236	39.0	35.0	41.0	33.0	41.0
21	37.50141430493744	39.0	35.0	41.0	33.0	41.0
22	37.44176682505897	39.0	35.0	41.0	33.0	41.0
23	37.352654535893805	39.0	35.0	41.0	32.0	41.0
24	37.28144003568627	39.0	35.0	41.0	32.0	41.0
25	37.153329857269675	39.0	35.0	41.0	32.0	41.0
26	37.07395448522993	39.0	35.0	41.0	32.0	41.0
27	36.97525594118855	39.0	35.0	41.0	32.0	41.0
28	37.04065517399274	39.0	35.0	41.0	32.0	41.0
29	36.986858411458606	39.0	35.0	41.0	32.0	41.0
30	36.944522319430554	39.0	35.0	41.0	32.0	41.0
31	36.80711243982742	39.0	35.0	41.0	31.0	41.0
32	36.59765794056522	39.0	35.0	41.0	31.0	41.0
33	36.46767925484224	39.0	35.0	41.0	30.0	41.0
34	36.32624728401754	39.0	35.0	41.0	30.0	41.0
35	36.142654993788874	39.0	35.0	41.0	30.0	41.0
36	36.00449623418231	39.0	35.0	41.0	29.0	41.0
37	35.963694829182984	39.0	35.0	41.0	29.0	41.0
38	35.93384007054538	39.0	35.0	41.0	29.0	41.0
39	35.8432625171526	39.0	35.0	41.0	29.0	41.0
40	35.77484856228333	39.0	35.0	41.0	28.0	41.0
41	35.765584311037784	38.0	35.0	41.0	28.0	41.0
42	35.59673771918034	38.0	35.0	41.0	27.0	41.0
43	35.625376840258134	38.0	35.0	41.0	27.0	41.0
44	35.39874684458598	38.0	35.0	40.0	27.0	41.0
45	35.313621196701384	38.0	34.0	40.0	26.0	41.0
46	35.294621505411286	38.0	34.0	40.0	26.0	41.0
47	35.22092994057704	38.0	34.0	40.0	26.0	41.0
48	35.18512254601476	38.0	34.0	40.0	26.0	41.0
49	35.17683021398471	38.0	34.0	40.0	26.0	41.0
50	34.675597589994844	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	2.0
14	9.0
15	12.0
16	30.0
17	70.0
18	203.0
19	405.0
20	655.0
21	1090.0
22	1755.0
23	2646.0
24	4036.0
25	6090.0
26	8710.0
27	11033.0
28	11704.0
29	12217.0
30	13903.0
31	16758.0
32	21247.0
33	27326.0
34	43913.0
35	68364.0
36	49820.0
37	63712.0
38	101650.0
39	209648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.459722220170725	23.08116116281715	27.498371518335745	15.960745098676387
2	34.24981277999915	24.36046090831611	28.12746026283177	13.262266048852972
3	28.602341764018608	24.899890843723366	32.560327675621224	13.937439716636804
4	25.331346167196696	27.694232442308913	32.159595634339766	14.814825756154626
5	22.338189482888758	31.92976185025059	31.390331914843333	14.341716752017323
6	20.45505907584958	40.64867483689334	28.70499888480394	10.191267202453137
7	86.09830564052874	3.8206173902639686	8.370174192147543	1.7109027770597522
8	86.40317513304807	3.190937813418098	8.194253859981595	2.2116331935522466
9	79.954683158767	6.160165787557366	10.436462627638251	3.4486884260373905
10	35.99262050395045	33.80654080952895	17.254667944833983	12.946170741686618
11	29.8834140065671	25.74906463853615	29.01075462584803	15.356766729048715
12	27.972514479085277	24.013346902782967	30.78930770696488	17.22483091116688
13	22.40894165678253	29.487704040259317	31.499192775302028	16.604161527656125
14	19.06335347579286	31.812777044981544	31.616029872483608	17.50783960674199
15	18.02496562094264	25.5656111939097	39.413982933807574	16.99544025134008
16	20.528322287230193	23.951161797961923	37.43041102729498	18.090104887512908
17	20.78843622924886	23.162843735183035	31.37999234872107	24.66872768684704
18	22.29993308823638	24.360904032578496	34.11465987997241	19.224502999212717
19	25.14626793360817	25.73119195995338	29.798481856277075	19.32405825016137
20	26.31921785613528	25.517458357397445	28.790374159356347	19.372949627110934
21	24.059874950333153	26.782725834587623	30.480302387996648	18.677096827082572
22	23.3262088799148	24.82411659485592	29.937475166577798	21.912199358651485
23	20.055656407355272	28.439419743549223	30.021373360255595	21.483550488839914
24	20.32418971035921	26.177713508347722	34.52440211458898	18.973694666704084
25	19.89184813836112	27.108569875526396	32.71069450865644	20.28888747745605
26	19.132628568812027	29.255802342945685	30.921949569504783	20.68961951873751
27	19.385652522632572	28.319037652268577	31.34823510991697	20.94707471518188
28	18.006206693835107	27.822295354137527	34.436958926812125	19.73453902521525
29	19.487571102980603	25.585994909979306	33.87950860473464	21.046925382305457
30	20.512812938046796	27.31019141491054	32.5532376874231	19.623757959619564
31	23.71084073966302	26.79217915218512	29.263483163493653	20.23349694465821
32	24.43918931893278	27.01388899146395	29.308386422081767	19.238535267521502
33	24.203447211345164	27.549183100422297	28.80071372547861	19.44665596275393
34	20.684154319501456	27.109012999788778	31.104812181781387	21.10202049892838
35	20.83525969297397	27.550660181296905	30.95562701344587	20.658453112283258
36	24.666364357447662	27.287887493703945	28.662606663702654	19.383141485145735
37	21.886202735258365	29.365992576191523	29.02360522945713	19.724199459092983
38	21.462575940420468	29.67056665253593	28.239718409302068	20.627138997741543
39	20.871300466314434	29.183573088177294	28.76688857345006	21.178237872058208
40	22.94881471645217	26.65673083598346	29.080472843129577	21.313981604434787
41	19.920651215415997	26.940625780083337	29.364220079141994	23.77450292535867
42	21.55740453257037	27.8862529560081	28.2259815571682	22.33036095425333
43	21.86197860891477	27.1709026884349	29.072053482144305	21.89506522050602
44	21.252091915788665	28.330115758828146	29.72802509855822	20.68976722682497
45	20.3064647398639	29.416065617840776	28.214164910171323	22.063304732124
46	21.722099050089287	29.117104448819887	29.016219825084082	20.144576676006743
47	21.374689628381223	27.79718497926917	29.611188001376636	21.216937390972966
48	21.88694127569567	26.285688120281648	29.959779087784394	21.86759151623829
49	21.284883111204987	25.884808370912733	30.869365490368693	21.960943027513586
50	20.3903629335417	28.623020896263135	29.919750196082482	21.06686597411268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	538.0
1	697.0
2	856.0
3	5336.0
4	9816.0
5	6786.5
6	3757.0
7	3864.0
8	3971.0
9	3948.0
10	3925.0
11	3976.5
12	4028.0
13	3882.0
14	3736.0
15	3565.0
16	3394.0
17	3359.5
18	3325.0
19	3267.5
20	3210.0
21	3473.5
22	3737.0
23	4156.0
24	4575.0
25	5193.0
26	5811.0
27	7091.5
28	8372.0
29	10113.5
30	11855.0
31	13799.0
32	15743.0
33	18223.5
34	20704.0
35	23201.0
36	25698.0
37	27597.0
38	29496.0
39	33118.5
40	36741.0
41	41345.5
42	45950.0
43	49682.5
44	53415.0
45	55182.5
46	56950.0
47	56534.5
48	56119.0
49	55653.5
50	55188.0
51	50941.0
52	46694.0
53	42147.5
54	37601.0
55	33358.5
56	29116.0
57	26600.5
58	24085.0
59	22182.0
60	20279.0
61	18012.0
62	15745.0
63	13688.5
64	11632.0
65	9815.5
66	7999.0
67	6362.0
68	4725.0
69	4044.5
70	3364.0
71	2716.5
72	2069.0
73	1695.0
74	1321.0
75	972.5
76	624.0
77	526.0
78	428.0
79	319.0
80	210.0
81	150.0
82	90.0
83	81.5
84	73.0
85	49.0
86	25.0
87	14.5
88	4.0
89	5.0
90	6.0
91	8.0
92	10.0
93	5.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	677011.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.027454856026722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.36327098347519	22.142206688181783
2	6.661601561890791	4.133850834608072
3	3.334735756807339	3.1040508945335343
4	2.335167488880474	2.898172153700008
5	1.8118458708346825	2.8108482981700766
6	1.4979763433801472	2.78870360217741
7	1.3172304100665586	2.8609214958328004
8	1.1348834929122067	2.8170037074546737
9	1.0315392273309425	2.8805433127408357
>10	9.463058939024567	51.14298522746253
>50	0.035324063524836885	0.7276656159668781
>100	0.012411157452810745	0.7021906543645733
>500	4.773522097234902E-4	0.12870779975591948
>1k	0.0	0.0
>5k	4.773522097234902E-4	0.8621497150508713
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5821	0.8598087771099732	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	869	0.12835832800353317	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05066387399909307	0.0
2	0.0	0.0	0.0	0.176068040253408	0.0
3	0.0	0.0	0.0	0.29645013153405186	0.0
4	0.0	0.0	0.0	0.40383391111813544	0.0
5	0.0	0.0	0.0	0.6138748114875534	0.0
6	0.0	0.0	0.0	1.0143114365940877	0.0
7	0.0	0.0	0.0	1.2395662699719798	0.0
8	0.0	0.0	0.0	1.845317136649183	0.0
9	0.0	0.0	0.0	2.2191663060127533	0.0
10	0.0	0.0	0.0	2.7411666870996187	0.0
11	0.0	0.0	0.0	3.082224661046866	0.0
12	0.0	0.0	0.0	3.3953658064640013	0.0
13	0.0	0.0	0.0	3.575126548903932	0.0
14	0.0	0.0	0.0	3.6706936814911426	0.0
15	0.0	0.0	0.0	3.7638974846789788	0.0
16	0.0	0.0	0.0	3.933761785259028	0.0
17	0.0	0.0	0.0	4.110863782124663	0.0
18	0.0	0.0	0.0	4.386339365239265	0.0
19	0.0	0.0	0.0	4.501256257283855	0.0
20	0.0	0.0	0.0	4.646748723432854	0.0
21	0.0	0.0	0.0	4.791798065319471	0.0
22	0.0	0.0	0.0	4.946743849065968	0.0
23	0.0	0.0	0.0	5.117346690083322	0.0
24	0.0	0.0	0.0	5.248954596010996	0.0
25	0.0	0.0	0.0	5.364314612317968	0.0
26	0.0	0.0	0.0	5.475834218350958	0.0
27	0.0	0.0	0.0	5.620440435975191	0.0
28	0.0	0.0	0.0	5.75130980146556	0.0
29	0.0	0.0	0.0	5.874498346407961	0.0
30	0.0	0.0	0.0	6.064598654970156	0.0
31	0.0	0.0	0.0	6.216294860792513	0.0
32	0.0	0.0	0.0	6.362082743116434	0.0
33	0.0	0.0	0.0	6.5069843769155895	0.0
34	0.0	0.0	0.0	6.645386854866464	0.0
35	0.0	0.0	0.0	6.8145126150092095	0.0
36	0.0	0.0	0.0	6.9659134046566455	0.0
37	0.0	0.0	0.0	7.121450020752986	0.0
38	0.0	0.0	0.0	7.283338084610147	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCAT	20	7.8541867E-4	44.0	23
ACCCGTA	20	7.8541867E-4	44.0	27
AAGTGCG	20	7.8541867E-4	44.0	1
CGTTTTT	3500	0.0	42.61714	1
TAGACGG	70	0.0	40.857143	2
CGTTCGA	60	3.6379788E-12	40.333332	14
ACGGGCG	55	7.8216544E-11	40.000004	5
TATACGG	50	1.3460522E-9	39.6	2
TAGCACG	45	2.3484972E-8	39.11111	1
TACGAAT	45	2.3484972E-8	39.11111	12
ACGGGAT	400	0.0	39.05	5
AGGGCGA	915	0.0	38.950817	6
CGAACGG	40	4.1211933E-7	38.5	2
TTATGCG	80	0.0	38.5	1
TTCACGG	40	4.1211933E-7	38.5	2
GGACCGT	40	4.1211933E-7	38.5	8
CGTTAGG	115	0.0	38.260868	2
TACGGGT	75	0.0	38.13333	4
TAGGGAC	695	0.0	37.985615	5
ATAGGGC	355	0.0	37.80282	4
>>END_MODULE