FastQCFastQC Report
Thu 26 May 2016
SRR1546509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546509_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1966136
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85780.4362872151265223No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC29380.14943015132218726No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26490.1347312698612914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC351.4467878E-744.033
CGTTTTT59550.040.6011731
CGACGGT2100.039.80952528
CGGTCTA2250.038.13333531
TATAGCG750.038.133331
TATGGGC11100.037.8558584
TAGCATA20300.037.71428330
AGGGCGC12700.037.5905536
GTTGATC21000.037.50476516
ACGGGTA2000.037.3999985
AGGGCGA25600.037.296886
AGGGTAC13900.037.1942446
TAGCCGT3550.037.18309844
TTTAAGC22250.037.1775344
GGTACCT22000.037.0000048
GGGTACC23300.036.9184577
TAGGGCG14500.036.8689655
CATATGC20850.036.8249433
GCGATAC1800.036.6666649
GGGCGAT48250.036.659077
Produced by FastQC (version 0.11.5)