##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546509_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1966136
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.430225070900487	31.0	31.0	33.0	30.0	34.0
2	31.832084352252338	31.0	31.0	34.0	30.0	34.0
3	31.979394609528537	33.0	31.0	34.0	30.0	34.0
4	35.64891950505967	37.0	35.0	37.0	33.0	37.0
5	35.55244449010648	37.0	35.0	37.0	33.0	37.0
6	35.58964588410974	37.0	35.0	37.0	33.0	37.0
7	36.01880541325727	37.0	35.0	37.0	35.0	37.0
8	36.01218328742264	37.0	35.0	37.0	35.0	37.0
9	37.732140604719106	39.0	38.0	39.0	35.0	39.0
10	37.08632770062702	39.0	37.0	39.0	33.0	39.0
11	36.89311064951763	39.0	37.0	39.0	33.0	39.0
12	36.459108627277054	39.0	35.0	39.0	32.0	39.0
13	36.30404407426546	39.0	35.0	39.0	32.0	39.0
14	37.37247474233725	40.0	35.0	41.0	32.0	41.0
15	37.52157480459134	40.0	35.0	41.0	32.0	41.0
16	37.56838794467931	39.0	35.0	41.0	33.0	41.0
17	37.49059881920681	39.0	35.0	41.0	32.0	41.0
18	37.46148587890156	39.0	36.0	41.0	32.0	41.0
19	37.448827039431656	39.0	36.0	41.0	32.0	41.0
20	37.308707027387726	39.0	35.0	41.0	32.0	41.0
21	37.214838647987726	39.0	35.0	41.0	32.0	41.0
22	37.14046993697282	39.0	35.0	41.0	32.0	41.0
23	37.04080694316161	39.0	35.0	41.0	32.0	41.0
24	36.9780198317919	39.0	35.0	41.0	32.0	41.0
25	36.798703141593464	38.0	35.0	41.0	31.0	41.0
26	36.67325200291333	38.0	35.0	41.0	31.0	41.0
27	36.618715592410695	38.0	35.0	41.0	31.0	41.0
28	36.67460745340099	38.0	35.0	41.0	31.0	41.0
29	36.56594660796608	38.0	35.0	40.0	31.0	41.0
30	36.54055619753669	38.0	35.0	40.0	31.0	41.0
31	36.45383635720011	38.0	35.0	40.0	31.0	41.0
32	36.31374431880602	38.0	35.0	41.0	30.0	41.0
33	36.27646561580684	38.0	35.0	41.0	30.0	41.0
34	36.185533452416315	38.0	35.0	41.0	30.0	41.0
35	36.017845154150066	38.0	35.0	41.0	30.0	41.0
36	35.89024360471503	38.0	35.0	41.0	29.0	41.0
37	35.843583048171645	38.0	35.0	40.0	29.0	41.0
38	35.78639168399338	38.0	35.0	40.0	29.0	41.0
39	35.67964474481928	38.0	35.0	40.0	29.0	41.0
40	35.558114494622956	38.0	35.0	40.0	28.0	41.0
41	35.53304552686081	38.0	35.0	40.0	28.0	41.0
42	35.350641562943764	38.0	34.0	40.0	27.0	41.0
43	35.360325531906234	37.0	34.0	40.0	28.0	41.0
44	35.11528907461132	37.0	34.0	40.0	27.0	41.0
45	35.014847904722764	37.0	34.0	40.0	27.0	41.0
46	35.002611721671336	37.0	34.0	40.0	27.0	41.0
47	34.88891867093629	37.0	34.0	40.0	27.0	41.0
48	34.84427526885221	36.0	34.0	40.0	27.0	41.0
49	34.816040701151906	36.0	34.0	40.0	27.0	41.0
50	34.311610692241025	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	8.0
13	6.0
14	19.0
15	57.0
16	110.0
17	291.0
18	649.0
19	1273.0
20	2304.0
21	3763.0
22	5864.0
23	8494.0
24	12077.0
25	17249.0
26	23653.0
27	29025.0
28	33286.0
29	37919.0
30	45411.0
31	55823.0
32	69792.0
33	90503.0
34	147950.0
35	225622.0
36	154806.0
37	199543.0
38	294577.0
39	506060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.40494452062319	23.825055845577314	28.11255172582161	13.657447907977883
2	32.78252369113835	25.484961365846516	28.354701811064952	13.37781313195018
3	30.812975297741357	25.309642873127803	29.682636399516614	14.194745429614228
4	26.146868782220555	28.420007568143813	29.57511586177152	15.858007787864114
5	23.107964047248004	32.40284497104982	28.96203517966204	15.527155802040143
6	20.370971285811358	41.51777903461409	26.96420796933681	11.147041710237746
7	87.51963241606889	3.9601533159455906	6.463235503546041	2.0569787644394895
8	87.26202053164175	3.340714986145414	6.140979057399895	3.2562854248129325
9	81.73595315888626	5.053210968112073	9.148960194004891	4.061875678996774
10	41.354463780735415	23.963754287597602	16.85066546769908	17.831116463967906
11	35.02936724621288	26.29772304662546	22.505665935621952	16.16724377153971
12	32.0530217645168	21.597234372393366	26.528632810751645	19.82111105233819
13	24.270701518104545	28.392186501849316	28.13437117269609	19.202740807350054
14	20.98344163374253	31.9930055703166	28.184825464769474	18.838727331171395
15	19.978170380889214	23.71122852132304	37.160450752135155	19.150150345652587
16	21.099303405257825	21.280165766762828	36.161079396338806	21.459451431640538
17	21.13246489561251	21.47659164981466	29.75277396884041	27.638169485732423
18	23.803236398702836	22.94266520728983	31.085031757721744	22.169066636285585
19	28.590951999251324	26.212581428751626	24.945883702856772	20.25058286914028
20	28.839307148640785	25.53633115918736	23.851961410604353	21.7724002815675
21	25.027058148571612	28.102226905971918	26.944016080271147	19.92669886518532
22	24.001340700745015	23.904195844031133	26.154396237086345	25.940067218137504
23	21.994358477745184	28.3699093043411	24.958141247604438	24.677590970309275
24	22.958177867655134	26.207393588236013	30.618939890221224	20.215488653887626
25	23.571156827401563	25.11225062762698	28.619790289176333	22.696802255795124
26	19.80773456159696	28.928415938673623	27.270595726846974	23.993253772882444
27	21.992476614028735	26.4192304092901	27.44250652040347	24.145786456277694
28	21.62068137707666	26.654819402116637	30.932092184874293	20.79240703593241
29	21.91364178266407	25.539484552441948	30.397337722314226	22.14953594257976
30	23.527060183018875	25.619387468618648	29.51092905068622	21.342623297676255
31	27.664617300125727	24.548098402145122	25.02548145194432	22.76180284578483
32	27.40049518446333	24.661925726399396	26.28968697994442	21.647892109192853
33	27.30340118893098	24.609487848246513	24.500899225689373	23.58621173713314
34	22.615932977169432	26.153989347634145	27.469361224248985	23.76071645094744
35	22.739779954184247	24.94379839441422	28.284360797015058	24.032060854386472
36	27.25482876057404	25.167994482579026	25.40088783278471	22.17628892406222
37	23.013718277881082	27.664413855399626	26.74458938750931	22.577278479209983
38	24.324461786977096	27.991146085520025	24.67535307832215	23.00903904918073
39	22.256649590872655	26.14915753538921	26.279514743639297	25.314678130098834
40	23.492016828947744	23.95999056016471	29.025052183572246	23.5229404273153
41	21.70917983293119	22.806814991434976	28.83406844694365	26.649936728690182
42	23.613676775156957	26.248133394638014	25.568475425911537	24.569714404293496
43	22.696191921616816	25.008493817314775	27.708408777419262	24.586905483649147
44	23.112897581855986	26.191321454873922	27.73612812135071	22.95965284191938
45	21.18230885350759	27.848175304251587	27.624640411446617	23.344875430794207
46	24.294606273421575	26.40147985693767	27.250810727233514	22.05310314240724
47	22.482320653301706	26.260289217022624	27.297806458963166	23.959583670712504
48	23.456312279516776	24.873711686271957	28.125877355381316	23.54409867882995
49	23.929168684160203	23.666216375672892	28.68743566060537	23.717179279561538
50	22.00773496848641	25.794604238974312	27.89278056044953	24.304880232089744
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1484.0
1	1887.5
2	2291.0
3	8768.0
4	15245.0
5	10806.5
6	6368.0
7	6656.0
8	6944.0
9	7348.0
10	7752.0
11	7748.5
12	7745.0
13	7657.0
14	7569.0
15	7390.0
16	7211.0
17	6891.5
18	6572.0
19	6655.5
20	6739.0
21	6983.0
22	7227.0
23	8213.0
24	9199.0
25	9788.0
26	10377.0
27	12295.0
28	14213.0
29	16409.5
30	18606.0
31	22987.5
32	27369.0
33	31087.0
34	34805.0
35	38921.5
36	43038.0
37	48531.0
38	54024.0
39	63695.0
40	73366.0
41	89669.0
42	105972.0
43	122390.5
44	138809.0
45	148461.5
46	158114.0
47	165003.0
48	171892.0
49	176118.5
50	180345.0
51	165301.5
52	150258.0
53	135796.0
54	121334.0
55	113138.5
56	104943.0
57	102334.5
58	99726.0
59	94028.5
60	88331.0
61	80803.5
62	73276.0
63	66724.0
64	60172.0
65	50777.0
66	41382.0
67	35857.5
68	30333.0
69	28548.0
70	26763.0
71	23363.0
72	19963.0
73	16726.0
74	13489.0
75	10040.5
76	6592.0
77	4785.5
78	2979.0
79	2319.5
80	1660.0
81	1368.5
82	1077.0
83	697.0
84	317.0
85	214.5
86	112.0
87	98.0
88	84.0
89	66.5
90	49.0
91	32.5
92	16.0
93	9.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1966136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.602750230975346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48228428859609	18.287950033891587
2	6.627141329318076	3.128375230825369
3	2.4369879541599806	1.7255885399380082
4	1.4796311542597553	1.3969345827185102
5	1.0267421411722242	1.2116969154852417
6	0.7867274662065568	1.1141359130832873
7	0.6552668761691468	1.0826270288996274
8	0.5560800528641727	1.0500014876944508
9	0.4700457747288175	0.9984935716244623
>10	6.730660226004681	39.1730249763364
>50	1.6398903164844245	25.35351533055949
>100	0.10225953015264176	3.7394011173481996
>500	0.004766330256378319	0.7680579583844385
>1k	0.0012999082517395415	0.5315554245942902
>5k	2.166513752899236E-4	0.43864188861662545
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8578	0.4362872151265223	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2938	0.14943015132218726	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2649	0.1347312698612914	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04562247982845541	0.0
2	0.0	0.0	0.0	0.18004858260059325	0.0
3	0.0	0.0	0.0	0.305777423331855	0.0
4	0.0	0.0	0.0	0.4745348236337669	0.0
5	0.0	0.0	0.0	0.780312246965622	0.0
6	0.0	0.0	0.0	1.4750759866051992	0.0
7	0.0	0.0	0.0	1.8752009016670261	0.0
8	0.0	0.0	0.0	3.096174425370371	0.0
9	0.0	0.0	0.0	3.742976070831316	0.0
10	0.0	0.0	0.0	4.676024445918288	0.0
11	0.0	0.0	0.0	5.217289139713631	0.0
12	0.0	0.0	0.0	5.676209580619042	0.0
13	0.0	0.0	0.0	5.919376889492893	0.0
14	0.0	0.0	0.0	6.032848185476488	0.0
15	0.0	0.0	0.0	6.131213710546981	0.0
16	0.0	0.0	0.0	6.3242827556181265	0.0
17	0.0	0.0	0.0	6.517606106596899	0.0
18	0.0	0.0	0.0	6.839659108016943	0.0
19	0.0	0.0	0.0	6.965591393474307	0.0
20	0.0	0.0	0.0	7.1193956064076955	0.0
21	0.0	0.0	0.0	7.284948752273495	0.0
22	0.0	0.0	0.0	7.4413468854646885	0.0
23	0.0	0.0	0.0	7.635178848258716	0.0
24	0.0	0.0	0.0	7.7929502333511005	0.0
25	0.0	0.0	0.0	7.916644626821339	0.0
26	0.0	0.0	0.0	8.040593326199206	0.0
27	0.0	0.0	0.0	8.184327025190527	0.0
28	0.0	0.0	0.0	8.315955762978756	0.0
29	0.0	0.0	0.0	8.456790374623118	0.0
30	0.0	0.0	0.0	8.644162967363396	0.0
31	0.0	0.0	0.0	8.786981165087257	0.0
32	0.0	0.0	0.0	8.931579504164514	0.0
33	0.0	0.0	0.0	9.079534681222459	0.0
34	0.0	0.0	0.0	9.2250993827487	0.0
35	0.0	0.0	0.0	9.38510865982821	0.0
36	0.0	0.0	0.0	9.530266471902248	0.0
37	0.0	0.0	0.0	9.690580916070912	0.0
38	0.0	0.0	0.0	9.874240642559823	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAC	35	1.4467878E-7	44.0	33
CGTTTTT	5955	0.0	40.601173	1
CGACGGT	210	0.0	39.809525	28
CGGTCTA	225	0.0	38.133335	31
TATAGCG	75	0.0	38.13333	1
TATGGGC	1110	0.0	37.855858	4
TAGCATA	2030	0.0	37.714283	30
AGGGCGC	1270	0.0	37.590553	6
GTTGATC	2100	0.0	37.504765	16
ACGGGTA	200	0.0	37.399998	5
AGGGCGA	2560	0.0	37.29688	6
AGGGTAC	1390	0.0	37.194244	6
TAGCCGT	355	0.0	37.183098	44
TTTAAGC	2225	0.0	37.17753	44
GGTACCT	2200	0.0	37.000004	8
GGGTACC	2330	0.0	36.918457	7
TAGGGCG	1450	0.0	36.868965	5
CATATGC	2085	0.0	36.82494	33
GCGATAC	180	0.0	36.666664	9
GGGCGAT	4825	0.0	36.65907	7
>>END_MODULE