FastQCFastQC Report
Thu 26 May 2016
SRR1546508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546508_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1561631
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.4332649646427357No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34260.21938601372539349No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC207.85735E-444.025
TAACGTG254.443577E-544.01
TACGCTA254.443577E-544.012
ATACCCG207.85735E-444.01
ACGACTA254.443577E-544.09
GTACGAC254.443577E-544.013
ACGTTAG850.041.4117621
TACGCGG850.041.4117622
CGTTTTT45450.040.9504971
TTTACGT557.8216544E-1140.00000415
ACGTAAG950.039.368421
GCGTAAG950.039.368421
TAATACG452.3519533E-839.111111
CGACGGT1650.038.66666828
CGGGATA2000.038.56
CGTAAGG2400.038.52
ACCGTTC404.1258136E-738.533
ATTGCGA404.1258136E-738.510
TAATCCG404.1258136E-738.51
CGTTTGG3150.038.41272
Produced by FastQC (version 0.11.5)