##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546508_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1561631
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.395867525683084	31.0	31.0	33.0	30.0	34.0
2	31.78568624726328	31.0	31.0	34.0	30.0	34.0
3	31.93259291087331	33.0	31.0	34.0	30.0	34.0
4	35.60488937527495	37.0	35.0	37.0	33.0	37.0
5	35.47896974381272	37.0	35.0	37.0	33.0	37.0
6	35.53195473194372	37.0	35.0	37.0	33.0	37.0
7	35.99326857625137	37.0	35.0	37.0	35.0	37.0
8	35.99234262127224	37.0	35.0	37.0	35.0	37.0
9	37.70456208925156	39.0	37.0	39.0	35.0	39.0
10	37.08160122333637	39.0	37.0	39.0	33.0	39.0
11	36.796724066056576	39.0	35.0	39.0	32.0	39.0
12	36.13465088743756	38.0	35.0	39.0	32.0	39.0
13	35.84484170716386	38.0	35.0	39.0	31.0	39.0
14	36.850931494059736	39.0	35.0	41.0	31.0	41.0
15	37.1019274079472	39.0	35.0	41.0	32.0	41.0
16	37.209787715535874	39.0	35.0	41.0	32.0	41.0
17	37.16000899060021	39.0	35.0	41.0	32.0	41.0
18	37.14129778417565	38.0	35.0	41.0	32.0	41.0
19	37.107651551486875	38.0	35.0	41.0	32.0	41.0
20	36.95430546652826	38.0	35.0	41.0	32.0	41.0
21	36.82659603965342	38.0	35.0	41.0	32.0	41.0
22	36.75678953606838	38.0	35.0	41.0	31.0	41.0
23	36.692353059077334	38.0	35.0	41.0	31.0	41.0
24	36.63724913247752	38.0	35.0	41.0	31.0	41.0
25	36.45641576018919	38.0	35.0	40.0	31.0	41.0
26	36.33556710900334	38.0	35.0	40.0	31.0	41.0
27	36.232953239273556	38.0	35.0	40.0	31.0	41.0
28	36.31987390106882	38.0	35.0	40.0	31.0	41.0
29	36.26344635832665	38.0	35.0	40.0	31.0	41.0
30	36.249901545243404	38.0	35.0	40.0	31.0	41.0
31	36.121376304645594	38.0	35.0	40.0	30.0	41.0
32	35.932623007611916	38.0	35.0	40.0	30.0	41.0
33	35.88973131296702	38.0	35.0	40.0	30.0	41.0
34	35.82237545233157	38.0	35.0	40.0	30.0	41.0
35	35.64940629380436	38.0	35.0	40.0	29.0	41.0
36	35.473729069159106	37.0	35.0	40.0	29.0	41.0
37	35.395698471662	37.0	34.0	40.0	28.0	41.0
38	35.36069020146245	37.0	34.0	40.0	28.0	41.0
39	35.28585049861331	37.0	34.0	40.0	28.0	41.0
40	35.15396915148329	37.0	34.0	40.0	27.0	41.0
41	35.18114074323576	37.0	34.0	40.0	28.0	41.0
42	34.98860037998733	37.0	34.0	40.0	27.0	41.0
43	34.98831734257325	36.0	34.0	40.0	27.0	41.0
44	34.74742176608943	36.0	34.0	40.0	26.0	41.0
45	34.64376027371383	36.0	34.0	40.0	26.0	41.0
46	34.65829251596568	36.0	34.0	40.0	26.0	41.0
47	34.55561909311483	35.0	34.0	40.0	26.0	41.0
48	34.532674492245604	35.0	34.0	40.0	26.0	41.0
49	34.54665538786051	36.0	34.0	40.0	26.0	41.0
50	34.00037781012288	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	4.0
13	5.0
14	10.0
15	31.0
16	87.0
17	252.0
18	570.0
19	1147.0
20	2085.0
21	3175.0
22	5103.0
23	7484.0
24	10834.0
25	15146.0
26	19977.0
27	25078.0
28	28476.0
29	32476.0
30	38728.0
31	47712.0
32	59349.0
33	77636.0
34	132481.0
35	225153.0
36	116054.0
37	142836.0
38	208636.0
39	361102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.24146357238041	24.243499264550973	28.714017588021758	13.801019575046858
2	29.997227257911764	26.244292025452875	30.490045343618306	13.268435373017057
3	29.465219376408385	25.799500650281658	30.638031647681174	14.097248325628781
4	25.896578641177076	28.038890109123088	30.619141141537277	15.445390108162554
5	22.774138064626023	33.067478809014425	29.06422836124539	15.094154765114165
6	20.470136671211062	42.31735922250519	25.692881352893227	11.519622753390525
7	87.15125404144769	4.436067163113437	6.388320928567633	2.0243578668712394
8	87.10892650056256	3.493975209252378	5.793750252140231	3.603348038044839
9	81.58457407671851	5.189766340447903	9.208513406816335	4.0171461760172535
10	47.27877456326111	24.32578502860151	14.206557118807195	14.188883289330192
11	40.07393551997879	21.5333199712352	22.917001519565122	15.475742989220887
12	36.48185775000624	20.25203136976661	25.101448421554128	18.164662458673014
13	22.705876100051807	33.228912592027186	27.266108318802583	16.799102989118428
14	18.30823030536663	36.491655198955456	27.434521983746478	17.765592511931434
15	16.38850663184837	22.413809664382946	44.13910840653138	17.05857529723731
16	17.75150467684107	19.086647229723283	42.29251340425491	20.869334689180736
17	17.949502795474732	19.760942245639335	29.27900381075939	33.010551148126545
18	21.961910336052497	22.423991327016434	33.648730077720025	21.965368259211044
19	27.824947122591702	25.628525560775884	25.11899417980304	21.42753313682938
20	28.667271589767363	24.16358281822018	24.153080977516456	23.016064614496
21	22.840094747094543	28.787658544175933	26.749020735372188	21.623225973357343
22	22.746346608129578	23.380427258424046	26.08208981507155	27.791136318374825
23	20.203428338704853	30.386883969388418	23.82278528026147	25.586902411645262
24	21.13386581080934	24.75719296043688	33.52085095646795	20.588090272285832
25	20.34193737188875	24.500346112493926	31.579547281015806	23.578169234601514
26	18.333588408529288	30.08143409038371	27.479090771123264	24.105886729963736
27	20.423518744184765	28.448269789726254	27.596980336583993	23.531231129504985
28	18.5657815450641	27.137844983866227	34.29702663433295	19.99934683673672
29	19.001800041110865	24.74201651990771	32.935053159165	23.321130279816423
30	21.54888062544865	26.884456059081817	30.026043284232962	21.540620031236575
31	28.12104780194553	24.46730373564562	23.259912232787386	24.151736229621466
32	27.936817340332	26.017541916112062	25.080957025059057	20.96468371849688
33	26.013891886111377	25.619112325510958	24.378742481418467	23.988253306959198
34	20.756375866001637	27.66396158887727	26.97481031050229	24.604852234618804
35	21.848823441645305	25.300279003170402	28.678477822225606	24.172419732958684
36	28.375589367782787	25.383333194589504	24.39763298756236	21.84344445006535
37	21.957748021139437	30.12382566688289	26.121343646482426	21.797082665495243
38	22.174572610302945	30.707958538220616	22.51139994019074	24.6060689112857
39	21.533255935621153	28.667655803451648	23.956171464321596	25.842916796605603
40	23.09514859784418	24.031925595739327	26.03624031541382	26.836685491002676
41	19.36590654258272	22.58260754301112	26.493902849008506	31.557583065397655
42	23.57938591126841	25.649721349025473	23.56395332828306	27.206939411423058
43	22.579854011607097	25.115792399100684	26.41289779723891	25.891455792053307
44	22.099714977481877	28.22356882003495	26.97084010243137	22.705876100051807
45	19.75037636932156	30.688235569094108	25.575119858660596	23.98626820292374
46	24.15404151172716	27.65506063852472	26.59053259060559	21.600365259142524
47	23.259015734190726	25.204994009468308	26.757025187128075	24.778965069212894
48	23.758941773056502	23.04782627906336	27.925162858575426	25.268069089304706
49	22.832090295338656	21.859261246734984	29.44427973061498	25.864368727311383
50	21.49041610982364	26.540392704806703	27.39936643163462	24.569824753735038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1526.0
1	1686.5
2	1847.0
3	6376.0
4	10905.0
5	7804.5
6	4704.0
7	5233.5
8	5763.0
9	6230.0
10	6697.0
11	6811.5
12	6926.0
13	6741.0
14	6556.0
15	6283.0
16	6010.0
17	5985.0
18	5960.0
19	5586.5
20	5213.0
21	4767.5
22	4322.0
23	4546.5
24	4771.0
25	5472.5
26	6174.0
27	7380.5
28	8587.0
29	9256.5
30	9926.0
31	12116.0
32	14306.0
33	18197.0
34	22088.0
35	24814.0
36	27540.0
37	30291.0
38	33042.0
39	43925.5
40	54809.0
41	77684.5
42	100560.0
43	116439.0
44	132318.0
45	140292.5
46	148267.0
47	153591.5
48	158916.0
49	158531.0
50	158146.0
51	144589.0
52	131032.0
53	112698.0
54	94364.0
55	86366.5
56	78369.0
57	74861.5
58	71354.0
59	67396.5
60	63439.0
61	56539.0
62	49639.0
63	46045.0
64	42451.0
65	35355.5
66	28260.0
67	24341.0
68	20422.0
69	17238.5
70	14055.0
71	12289.5
72	10524.0
73	7996.5
74	5469.0
75	4157.5
76	2846.0
77	2247.5
78	1649.0
79	1318.5
80	988.0
81	713.5
82	439.0
83	379.0
84	319.0
85	184.0
86	49.0
87	39.5
88	30.0
89	22.5
90	15.0
91	23.5
92	32.0
93	18.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1561631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.109015441767234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.78179526979542	17.638969435918195
2	7.131440160244545	3.1533824124977134
3	2.4540924656919714	1.627727046585253
4	1.3130756731806352	1.1612324133823828
5	0.8262464922333316	0.9133748227746371
6	0.6307267847767251	0.8366848934498887
7	0.47635332174286515	0.7372192062305086
8	0.4079830294682168	0.7216082478793425
9	0.31733539564550095	0.6314375846291126
>10	4.133175509298628	22.11309940525971
>50	1.8659335913718118	30.168387161857822
>100	0.654294982002135	18.454828105018407
>500	0.0060959159812170515	0.959274975846166
>1k	0.0011611268535651525	0.44854303915092353
>5k	2.9028171339128814E-4	0.43423124951998116
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6766	0.4332649646427357	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3426	0.21938601372539349	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06326718667854314	0.0
2	0.0	0.0	0.0	0.22444482723511508	0.0
3	0.0	0.0	0.0	0.35693451269858245	0.0
4	0.0	0.0	0.0	0.6133971469572518	0.0
5	0.0	0.0	0.0	1.0368006270367327	0.0
6	0.0	0.0	0.0	1.8910997540392065	0.0
7	0.0	0.0	0.0	2.418048822032862	0.0
8	0.0	0.0	0.0	4.183062452013312	0.0
9	0.0	0.0	0.0	5.186180346061265	0.0
10	0.0	0.0	0.0	6.581324269305617	0.0
11	0.0	0.0	0.0	7.295001187860641	0.0
12	0.0	0.0	0.0	7.995742912378148	0.0
13	0.0	0.0	0.0	8.31636923191202	0.0
14	0.0	0.0	0.0	8.451164199481184	0.0
15	0.0	0.0	0.0	8.565595841783367	0.0
16	0.0	0.0	0.0	8.814310166742336	0.0
17	0.0	0.0	0.0	9.070388587316723	0.0
18	0.0	0.0	0.0	9.51665278161102	0.0
19	0.0	0.0	0.0	9.675589175675944	0.0
20	0.0	0.0	0.0	9.885497918522365	0.0
21	0.0	0.0	0.0	10.084968856279108	0.0
22	0.0	0.0	0.0	10.281045906491354	0.0
23	0.0	0.0	0.0	10.507924087060259	0.0
24	0.0	0.0	0.0	10.67845092726771	0.0
25	0.0	0.0	0.0	10.80998007852047	0.0
26	0.0	0.0	0.0	10.945031188545821	0.0
27	0.0	0.0	0.0	11.09564295278462	0.0
28	0.0	0.0	0.0	11.239979226846803	0.0
29	0.0	0.0	0.0	11.393984878630098	0.0
30	0.0	0.0	0.0	11.613755106039775	0.0
31	0.0	0.0	0.0	11.773395891859217	0.0
32	0.0	0.0	0.0	11.924840119080628	0.0
33	0.0	0.0	0.0	12.084801082970305	0.0
34	0.0	0.0	0.0	12.241112016859297	0.0
35	0.0	0.0	0.0	12.439366277949144	0.0
36	0.0	0.0	0.0	12.594396499557194	0.0
37	0.0	0.0	0.0	12.760056633097063	0.0
38	0.0	0.0	0.0	12.93288875541021	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAC	20	7.85735E-4	44.0	25
TAACGTG	25	4.443577E-5	44.0	1
TACGCTA	25	4.443577E-5	44.0	12
ATACCCG	20	7.85735E-4	44.0	1
ACGACTA	25	4.443577E-5	44.0	9
GTACGAC	25	4.443577E-5	44.0	13
ACGTTAG	85	0.0	41.411762	1
TACGCGG	85	0.0	41.411762	2
CGTTTTT	4545	0.0	40.950497	1
TTTACGT	55	7.8216544E-11	40.000004	15
ACGTAAG	95	0.0	39.36842	1
GCGTAAG	95	0.0	39.36842	1
TAATACG	45	2.3519533E-8	39.11111	1
CGACGGT	165	0.0	38.666668	28
CGGGATA	200	0.0	38.5	6
CGTAAGG	240	0.0	38.5	2
ACCGTTC	40	4.1258136E-7	38.5	33
ATTGCGA	40	4.1258136E-7	38.5	10
TAATCCG	40	4.1258136E-7	38.5	1
CGTTTGG	315	0.0	38.4127	2
>>END_MODULE