FastQCFastQC Report
Thu 26 May 2016
SRR1546507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546507_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2246053
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360451.6048152024907694No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC27640.12306031959174604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG750.044.01
CGTTTTT214450.042.5740281
CGGTCTA1900.038.2105331
TCGATAG750.038.133331
GTTTTTT247350.037.7384262
CATATGC18400.037.42391633
TACGGGA5050.036.594064
GCGCGAC19100.036.513099
GGGCGAT50200.036.4183277
TAGCATA18850.036.2970830
ATAGGGC13850.036.057764
AGGGTAC12400.036.016136
GGTACCT20300.035.9802978
GTTGATC19750.035.97974416
GTACGAG1900.035.8947371
GTAGGGA17900.035.8882684
ATATGCT18950.035.8733534
GTAGGGC12100.035.818184
TATGGGC9850.035.7360424
TAGGGCC5550.035.6756745
Produced by FastQC (version 0.11.5)