##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546507_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2246053
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.404713512993684	31.0	31.0	33.0	30.0	34.0
2	31.797862294433834	31.0	31.0	34.0	30.0	34.0
3	31.883982256874614	31.0	31.0	34.0	30.0	34.0
4	35.60642202120787	37.0	35.0	37.0	33.0	37.0
5	35.515964672249495	37.0	35.0	37.0	33.0	37.0
6	35.542258797989184	37.0	35.0	37.0	33.0	37.0
7	35.98738008408528	37.0	35.0	37.0	35.0	37.0
8	35.968440637865626	37.0	35.0	37.0	35.0	37.0
9	37.660545855329325	39.0	37.0	39.0	35.0	39.0
10	37.08959939947989	39.0	37.0	39.0	33.0	39.0
11	36.89453098390822	39.0	37.0	39.0	32.0	39.0
12	36.648659670987286	39.0	35.0	39.0	32.0	39.0
13	36.626454941179034	39.0	35.0	39.0	32.0	39.0
14	37.712905706143175	40.0	36.0	41.0	32.0	41.0
15	37.78700502615032	40.0	36.0	41.0	33.0	41.0
16	37.794270215351105	40.0	36.0	41.0	33.0	41.0
17	37.68438723396109	40.0	36.0	41.0	32.0	41.0
18	37.547280941277876	39.0	36.0	41.0	32.0	41.0
19	37.431366490461265	39.0	36.0	41.0	32.0	41.0
20	37.197482873289275	39.0	35.0	41.0	32.0	41.0
21	37.09316298413261	39.0	35.0	41.0	32.0	41.0
22	37.0412657225809	39.0	35.0	41.0	32.0	41.0
23	36.92210557809633	38.0	35.0	41.0	31.0	41.0
24	36.84037197697472	38.0	35.0	41.0	31.0	41.0
25	36.65480289200656	38.0	35.0	41.0	31.0	41.0
26	36.5545528088607	38.0	35.0	40.0	31.0	41.0
27	36.482992164476975	38.0	35.0	40.0	31.0	41.0
28	36.48790077527111	38.0	35.0	40.0	31.0	41.0
29	36.33349435654457	38.0	35.0	40.0	30.0	41.0
30	36.24704715338418	38.0	35.0	40.0	30.0	41.0
31	36.06788530813832	38.0	35.0	40.0	30.0	41.0
32	35.73130108683989	38.0	35.0	40.0	28.0	41.0
33	35.46189916266446	38.0	35.0	40.0	26.0	41.0
34	35.141038969249614	38.0	34.0	40.0	24.0	41.0
35	34.84495557317659	38.0	34.0	40.0	23.0	41.0
36	34.68854163281098	38.0	34.0	40.0	23.0	41.0
37	34.65133814740792	38.0	34.0	40.0	22.0	41.0
38	34.539833654860324	38.0	34.0	40.0	22.0	41.0
39	34.42018198145814	38.0	34.0	40.0	22.0	41.0
40	34.268222521908434	37.0	33.0	40.0	21.0	41.0
41	34.20732102047458	37.0	33.0	40.0	21.0	41.0
42	33.99735001800937	37.0	33.0	40.0	20.0	41.0
43	33.99462568336544	37.0	33.0	40.0	20.0	41.0
44	33.719131293874185	36.0	33.0	40.0	18.0	41.0
45	33.61086937841627	36.0	33.0	40.0	20.0	41.0
46	33.594961472414056	36.0	33.0	40.0	20.0	41.0
47	33.49714543690643	36.0	33.0	40.0	20.0	41.0
48	33.43240876328386	36.0	33.0	40.0	20.0	41.0
49	33.392762770958655	35.0	33.0	40.0	20.0	41.0
50	32.911376534747845	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	6.0
12	7.0
13	20.0
14	24.0
15	85.0
16	186.0
17	490.0
18	1052.0
19	2126.0
20	3887.0
21	6007.0
22	9113.0
23	13180.0
24	19692.0
25	29975.0
26	45211.0
27	56000.0
28	57388.0
29	56895.0
30	59198.0
31	67209.0
32	80545.0
33	100931.0
34	153717.0
35	198909.0
36	190407.0
37	238169.0
38	337862.0
39	517754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.26686725558123	21.288990063903213	24.463848359767113	19.980294320748442
2	39.252413010734834	22.498801230425105	24.969223789465342	13.27956196937472
3	29.34396472389565	23.696368696553467	33.632331917367935	13.327334662182949
4	25.25728466781505	25.963991054529878	33.39890020404683	15.379824073608237
5	22.357753801891587	29.593290986454907	32.855101816386345	15.193853395267165
6	19.980650501123527	37.80529666931279	31.531669110212444	10.682383719351234
7	80.43216255359958	3.8540942711503248	13.322348136931764	2.39139503831833
8	79.52278953346159	3.544306389920451	13.474392634546023	3.458511442071937
9	73.92759654380373	5.10651351504172	16.32997974669342	4.635910194461128
10	37.212434434984395	25.100431735137153	22.16368002001734	15.52345380986112
11	29.30701100998062	25.02082542130573	28.924517809686595	16.74764575902706
12	25.801216623116197	20.785662671361717	33.75490248894394	19.65821821657815
13	24.14163868795616	21.809191501714338	35.000376215521186	19.048793594808313
14	21.016111374041486	24.770831320543195	35.14075580585142	19.072301499563903
15	20.219647532805325	21.549402440636975	38.55354259227187	19.677407434285836
16	22.89883631419205	20.722084474409108	36.285653099014134	20.093426112384705
17	22.141819449496516	20.86139552361409	34.64063403668568	22.356150990203705
18	24.29582026782093	20.987483376394056	34.03120941491585	20.68548694086916
19	26.675416831214584	24.545547233302152	29.82405134696287	18.954984588520396
20	27.641155395709717	23.507726665399257	28.97269120541679	19.87842673347423
21	25.627979393184397	25.03734328620028	30.778570229642842	18.556107090972475
22	24.069289549267094	21.804383066650697	30.716194141456143	23.410133242626063
23	22.99264532047997	25.31592086206336	29.714882061999425	21.97655175545724
24	22.404279863387018	23.49512678463064	32.76797119213126	21.33262215985108
25	24.478896980614437	23.24695810829041	30.49349236193447	21.780652549160685
26	21.273318127399488	23.62642377539622	31.77057709680048	23.32968100040382
27	22.618611404094207	21.823750374545924	31.710249045770517	23.847389175589356
28	22.687087081204226	24.297868304977666	32.04247629063072	20.97256832318739
29	23.669031852765716	23.87583908304924	31.073354012572274	21.381775051612763
30	24.217994855864934	21.876019844589596	32.81374037032964	21.092244929215827
31	25.47121550560027	22.723150344181548	29.91759321796948	21.888040932248703
32	25.48844573124499	23.157512311597277	30.272081736272476	21.08196022088526
33	25.186939043735833	23.029999737316974	29.39712464487704	22.385936574070158
34	22.78922180375975	25.778955349673403	30.60079169992872	20.831031146638125
35	23.148874937501475	25.052525474688263	29.732824648394317	22.065774939415945
36	24.518611092436377	27.664885913199733	25.78011293589243	22.03639005847146
37	24.75462511347684	26.59256037146051	25.7897743285666	22.863040186496043
38	24.092752931475793	27.46333234344871	26.033846930593356	22.410067794482142
39	24.411133664254585	24.95938430660363	25.320729297127002	25.30875273201478
40	24.920694213360058	24.125477003436696	28.874118286612116	22.079710496591133
41	22.987881407963215	24.33370895522056	28.44011249957147	24.238297137244757
42	24.108870093448374	26.308996270346245	26.608187785417353	22.973945850788027
43	23.405057672281107	24.380591197091075	28.487261876723302	23.727089253904516
44	24.142395571253218	24.93707851061395	28.896067902226708	22.024458015906127
45	22.863529934511785	24.455389075858854	29.44765773559217	23.233423254037195
46	24.105219244603756	24.884764518023395	28.166610494053344	22.843405743319504
47	22.739490118888554	25.788839355082004	28.737077887298295	22.734592638731144
48	23.24673549555598	25.09882892344927	28.531294675593138	23.12314090540161
49	24.621280085554524	24.00544421703317	28.25832694063764	23.114948756774663
50	22.271869808949297	24.049209880621696	30.18156739845409	23.497352911974918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1492.0
1	2380.0
2	3268.0
3	31446.5
4	59625.0
5	40865.5
6	22106.0
7	21986.0
8	21866.0
9	21737.0
10	21608.0
11	21203.0
12	20798.0
13	19967.0
14	19136.0
15	18028.0
16	16920.0
17	16142.5
18	15365.0
19	14472.5
20	13580.0
21	13137.0
22	12694.0
23	12600.5
24	12507.0
25	13843.0
26	15179.0
27	15989.5
28	16800.0
29	18693.5
30	20587.0
31	23911.0
32	27235.0
33	31252.5
34	35270.0
35	40339.0
36	45408.0
37	49592.0
38	53776.0
39	60040.0
40	66304.0
41	75594.0
42	84884.0
43	93596.5
44	102309.0
45	116386.5
46	130464.0
47	148039.5
48	165615.0
49	175773.5
50	185932.0
51	172392.0
52	158852.0
53	148045.5
54	137239.0
55	130432.5
56	123626.0
57	122308.0
58	120990.0
59	118971.5
60	116953.0
61	110191.5
62	103430.0
63	94365.0
64	85300.0
65	72760.0
66	60220.0
67	53165.0
68	46110.0
69	41241.5
70	36373.0
71	32004.5
72	27636.0
73	22685.0
74	17734.0
75	14056.5
76	10379.0
77	8101.0
78	5823.0
79	4137.0
80	2451.0
81	1862.0
82	1273.0
83	911.0
84	549.0
85	377.0
86	205.0
87	157.5
88	110.0
89	75.0
90	40.0
91	26.5
92	13.0
93	12.5
94	12.0
95	7.5
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2246053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.46740800601049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.56060786636016	23.233874527552388
2	7.213836174922013	4.684291247594221
3	3.548218775472329	3.4560440003354107
4	2.4932396210589283	3.2379611213468475
5	1.9719631272914329	3.2012265713289674
6	1.6045263620809758	3.1256887232449655
7	1.4277095010653678	3.2447818819602876
8	1.261358212099188	3.2762425371165
9	1.067619507220621	3.1196554322496977
>10	7.713325982862357	42.00297282712231
>50	0.09525755092879687	2.066703034123062
>100	0.03682269927601644	2.2321244197097787
>500	0.004411695489364862	1.016261672318286
>1k	9.650583882985622E-4	0.4887491719539885
>5k	0.0	0.0
>10k+	1.3786548404265175E-4	1.6134228320433663
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36045	1.6048152024907694	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2764	0.12306031959174604	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04309782538524247	0.0
2	0.0	0.0	0.0	0.14750319783193006	0.0
3	0.0	0.0	0.0	0.3060925098383698	0.0
4	0.0	0.0	0.0	0.4371223653226349	0.0
5	0.0	0.0	0.0	0.7113812541378142	0.0
6	0.0	0.0	0.0	1.4790835300858884	0.0
7	0.0	0.0	0.0	2.000754211944242	0.0
8	0.0	0.0	0.0	3.348407183623895	0.0
9	0.0	0.0	0.0	4.2077813836093805	0.0
10	0.0	0.0	0.0	5.326766554484689	0.0
11	0.0	0.0	0.0	5.828891838260272	0.0
12	0.0	0.0	0.0	6.288854270135211	0.0
13	0.0	0.0	0.0	6.611152987039932	0.0
14	0.0	0.0	0.0	6.798860044709541	0.0
15	0.0	0.0	0.0	6.915019369534023	0.0
16	0.0	0.0	0.0	7.075077925587687	0.0
17	0.0	0.0	0.0	7.225074386045209	0.0
18	0.0	0.0	0.0	7.501025131642041	0.0
19	0.0	0.0	0.0	7.619855809279656	0.0
20	0.0	0.0	0.0	7.765355492501735	0.0
21	0.0	0.0	0.0	7.901149260502757	0.0
22	0.0	0.0	0.0	8.039080110754288	0.0
23	0.0	0.0	0.0	8.199183189354837	0.0
24	0.0	1.335676406567432E-4	0.0	8.334442686793233	0.0
25	0.0	1.335676406567432E-4	0.0	8.456657077994153	0.0
26	0.0	1.335676406567432E-4	0.0	8.57455278214717	0.0
27	0.0	1.335676406567432E-4	0.0	8.73799505176414	0.0
28	0.0	1.335676406567432E-4	0.0	8.86412742709099	0.0
29	0.0	1.335676406567432E-4	0.0	9.004061791952372	0.0
30	0.0	1.335676406567432E-4	0.0	9.205437271515855	0.0
31	0.0	1.335676406567432E-4	0.0	9.356546795645517	0.0
32	0.0	1.335676406567432E-4	0.0	9.546925206128261	0.0
33	0.0	1.335676406567432E-4	0.0	9.71611088429347	0.0
34	0.0	1.335676406567432E-4	0.0	9.877282504019274	0.0
35	0.0	1.335676406567432E-4	0.0	10.052300635826493	0.0
36	0.0	1.335676406567432E-4	0.0	10.20959879397325	0.0
37	0.0	1.335676406567432E-4	0.0	10.384171700311613	0.0
38	0.0	1.335676406567432E-4	0.0	10.591335110970222	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	75	0.0	44.0	1
CGTTTTT	21445	0.0	42.574028	1
CGGTCTA	190	0.0	38.21053	31
TCGATAG	75	0.0	38.13333	1
GTTTTTT	24735	0.0	37.738426	2
CATATGC	1840	0.0	37.423916	33
TACGGGA	505	0.0	36.59406	4
GCGCGAC	1910	0.0	36.51309	9
GGGCGAT	5020	0.0	36.418327	7
TAGCATA	1885	0.0	36.29708	30
ATAGGGC	1385	0.0	36.05776	4
AGGGTAC	1240	0.0	36.01613	6
GGTACCT	2030	0.0	35.980297	8
GTTGATC	1975	0.0	35.979744	16
GTACGAG	190	0.0	35.894737	1
GTAGGGA	1790	0.0	35.888268	4
ATATGCT	1895	0.0	35.87335	34
GTAGGGC	1210	0.0	35.81818	4
TATGGGC	985	0.0	35.736042	4
TAGGGCC	555	0.0	35.675674	5
>>END_MODULE