FastQCFastQC Report
Thu 26 May 2016
SRR1546506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546506_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1663159
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111760.6719742369791463No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19470.11706637789892609No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC19060.11460118966376635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG900.041.5555571
CGTTTTT64150.041.119251
ATTAACG650.040.6153871
TAGCCGT2350.038.38297744
ATCGCAT1450.037.93103810
AGGGCGA18700.037.647066
TAACGGG3750.037.5466653
AACCCGA1650.037.33333224
GTTTACG651.0913936E-1137.230771
CTACGGG3500.037.0857123
TATGGGC6200.036.9032254
CGCCAGT1850.036.86486426
TTTAAGC14300.036.7692344
GGGCGAT40050.036.7490657
TAGGGCG9650.036.7046665
CAACCCG1500.036.66666823
TAACGCC2100.036.66666812
ATAACGC2100.036.66666811
GTTGATC14950.036.49498416
CATATGC14900.036.469833
Produced by FastQC (version 0.11.5)