FastQCFastQC Report
Thu 26 May 2016
SRR1546505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546505_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1084004
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61650.5687248386537319No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14220.1311803277478681No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC11100.1023981461323021No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAT207.856285E-444.036
TCTAGCG254.4426728E-544.01
CGTACGT207.856285E-444.017
TTACGAG502.7284841E-1144.01
CCGGTAT454.802132E-1044.042
GTACGTC207.856285E-444.018
TAGCATA11750.040.4425530
CGTTTTT35200.040.43751
AGGGTAC7600.039.9473726
TCGTAAA501.3478711E-939.615
ATGGTCG1450.039.44827744
CATATGC12050.039.2531133
TAGCCGT1350.039.1111144
ATCGTCC452.35068E-839.1111116
GTAGCAT12500.038.89629
GTTGATC12650.038.78260816
GCCAGTA12500.038.7225
ATCCTGC12850.038.69260820
AGCATAT12350.038.6558731
TTGATCC12800.038.32812517
Produced by FastQC (version 0.11.5)