FastQCFastQC Report
Thu 26 May 2016
SRR1546504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546504_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1409073
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136910.9716317039642375No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC15280.10844008791595611No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATA254.443355E-544.025
TCGTAAG351.4463876E-744.01
ACATACG850.044.017
CGTTTTT76250.041.5763931
CATACGA900.041.55555718
ATAACGC1500.041.06666611
TACGCGG452.3515895E-839.111112
TAACGCC1550.038.32258212
GCGTAAG750.038.133331
CGTTGAT1450.037.93103425
GGTACCT14000.037.3999988
TAGCATA12650.037.39130430
TTTAAGC13300.037.38345744
CATATGC12600.037.36507833
TACGGGA3800.037.0526354
GTAGCAT13050.036.9195429
GTTGATC13500.036.66666816
CGCATCG1500.036.66666821
TCTAGCG301.3012446E-436.6666641
TATCGAT301.3012446E-436.66666432
Produced by FastQC (version 0.11.5)