FastQCFastQC Report
Thu 26 May 2016
SRR1546503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546503_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1806200
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208151.1524194441368618No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC23130.12805890820507143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126650.042.1239621
CGGTAAG452.3521352E-839.1111071
TTGTACG850.038.8235321
GTTGATC14950.037.81939716
GCGCGAC13750.037.449
TAGGGTC5300.037.358495
TTGATCC15250.037.0754117
GCCAGTA15150.036.73927325
GTTTTTT149950.036.7375762
TCGTTAG900.036.6666641
AGGGCGA21250.036.649416
AGGGTAC11050.036.434396
ACCCGCT13250.036.36226734
TATACTA12050.036.33194744
TGATCCT15650.036.2683718
TAGCATA15350.036.26058630
TAGTCGG850.036.2352942
GGTACCT16550.036.1570978
GGGCGAT43450.036.1012657
ATATGCT15150.036.01320334
Produced by FastQC (version 0.11.5)