FastQCFastQC Report
Thu 26 May 2016
SRR1546502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546502_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2013801
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143390.7120365915003518No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20520.10189686071265235No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAA207.8578957E-444.026
ATCGCTA254.4440363E-544.038
GTAATAG2650.041.5094341
CGTTTTT80200.041.256861
AACGTTG950.039.368421
CGGTCTA2400.038.531
CACGACG2250.038.13333526
TACGGGA3850.037.7142874
CATACGA2100.037.71428718
AGGGCGA22550.037.3658526
GGGCGAT44400.037.360367
TAACGCC1900.037.0526312
ATTAGCG1200.036.6666641
TATTCGA301.301443E-436.66666427
TAATGCG601.9826984E-1036.6666641
TCTATCG301.301443E-436.6666641
ATAGGGC12250.036.6367344
TTGATCC17000.036.49411817
ACGGGAT3500.036.457145
CATATGC16150.036.37151733
Produced by FastQC (version 0.11.5)