##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546502_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2013801
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.377378400348395	31.0	31.0	33.0	30.0	34.0
2	31.76607221865517	31.0	31.0	34.0	30.0	34.0
3	31.904366419522088	31.0	31.0	34.0	30.0	34.0
4	35.593907739642596	37.0	35.0	37.0	33.0	37.0
5	35.48501962209772	37.0	35.0	37.0	33.0	37.0
6	35.518593445926385	37.0	35.0	37.0	33.0	37.0
7	35.96694410222261	37.0	35.0	37.0	35.0	37.0
8	35.9473840761823	37.0	35.0	37.0	35.0	37.0
9	37.631785365088206	39.0	37.0	39.0	35.0	39.0
10	37.04184574344734	39.0	37.0	39.0	33.0	39.0
11	36.803921042843854	39.0	37.0	39.0	32.0	39.0
12	36.36991738508423	38.0	35.0	39.0	32.0	39.0
13	36.229356823241226	39.0	35.0	39.0	32.0	39.0
14	37.24794704144054	39.0	35.0	41.0	32.0	41.0
15	37.40869728438907	39.0	35.0	41.0	32.0	41.0
16	37.462650976933666	39.0	35.0	41.0	32.0	41.0
17	37.398723607744756	39.0	35.0	41.0	32.0	41.0
18	37.34902405947758	39.0	35.0	41.0	32.0	41.0
19	37.323393920253295	39.0	35.0	41.0	32.0	41.0
20	37.158140253182914	39.0	35.0	41.0	32.0	41.0
21	37.03856885561185	39.0	35.0	41.0	32.0	41.0
22	36.965465803224845	39.0	35.0	41.0	31.0	41.0
23	36.86934657396635	38.0	35.0	41.0	31.0	41.0
24	36.805729563149484	38.0	35.0	41.0	31.0	41.0
25	36.608008934348526	38.0	35.0	41.0	31.0	41.0
26	36.50553604849734	38.0	35.0	40.0	31.0	41.0
27	36.411728864967294	38.0	35.0	40.0	31.0	41.0
28	36.46666924884832	38.0	35.0	40.0	31.0	41.0
29	36.34987071711654	38.0	35.0	40.0	30.0	41.0
30	36.306830714653536	38.0	35.0	40.0	30.0	41.0
31	36.18016626270421	38.0	35.0	40.0	30.0	41.0
32	36.00499850779695	38.0	35.0	40.0	30.0	41.0
33	35.91154538109774	38.0	35.0	40.0	29.0	41.0
34	35.78136221006942	38.0	35.0	40.0	29.0	41.0
35	35.581890166903285	38.0	35.0	40.0	28.0	41.0
36	35.43448632709985	38.0	34.0	40.0	27.0	41.0
37	35.38004152346731	38.0	34.0	40.0	27.0	41.0
38	35.30125965773182	38.0	34.0	40.0	27.0	41.0
39	35.17296694161936	38.0	34.0	40.0	26.0	41.0
40	35.03732295296308	37.0	34.0	40.0	26.0	41.0
41	34.988801276789516	37.0	34.0	40.0	26.0	41.0
42	34.79224163658673	37.0	34.0	40.0	25.0	41.0
43	34.79494895473783	37.0	34.0	40.0	25.0	41.0
44	34.53375134881748	36.0	34.0	40.0	24.0	41.0
45	34.43442077941167	36.0	33.0	40.0	24.0	41.0
46	34.43010456345984	36.0	34.0	40.0	24.0	41.0
47	34.31350466108618	36.0	33.0	40.0	24.0	41.0
48	34.25553418634711	36.0	33.0	40.0	24.0	41.0
49	34.226046168414854	36.0	33.0	40.0	24.0	41.0
50	33.68965255256105	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	7.0
12	9.0
13	9.0
14	27.0
15	70.0
16	175.0
17	409.0
18	904.0
19	1726.0
20	3047.0
21	4768.0
22	7444.0
23	10747.0
24	14857.0
25	21087.0
26	28574.0
27	35474.0
28	39308.0
29	43694.0
30	50658.0
31	61359.0
32	75231.0
33	96014.0
34	154851.0
35	226600.0
36	161190.0
37	208914.0
38	294462.0
39	472181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.555797221274595	23.134609626273896	26.5315689087452	14.778024243706305
2	34.12526858413518	24.917953660763896	27.354391024733825	13.602386730367103
3	30.592794422090364	25.134310689089933	30.113452123620952	14.159442765198746
4	25.921677464655147	28.041896890507058	30.165592330125968	15.87083331471183
5	23.067373588552194	31.859602810804045	29.409460021124232	15.663563579519527
6	20.91889913650852	40.250104156269664	27.57213845856666	11.258858248655155
7	84.91171669891911	4.485497822277375	8.109738747771006	2.49304673103251
8	83.80406008339453	4.0649001564702765	8.183777841008123	3.947261919127064
9	77.9871496736768	5.590820542844104	11.584560738623132	4.837469044855972
10	43.84102500693962	22.997605026514538	17.239339934780052	15.9220300317658
11	35.40036974855013	24.272209617534205	24.062109413988768	16.265311219926897
12	31.025856080119134	21.100595341843608	28.298625335869833	19.574923242167426
13	24.74618892333453	26.552176704649565	30.21837808204485	18.483256289971052
14	20.402909721467015	29.689080500009684	30.974063474990825	18.933946303532473
15	19.26143645772348	21.862239615533014	39.538514480825064	19.337809445918438
16	21.604617338058727	20.096325307217544	37.3367080461277	20.962349308596036
17	21.239387605826	20.751106986241442	30.732927434240025	27.276577973692532
18	23.91328636742161	22.079937392026324	32.002367661948725	22.00440857860335
19	28.24390294770933	25.208548411685168	25.885427606799283	20.66212103380622
20	28.698366919074925	24.499590575235587	25.126663458802533	21.675379046886956
21	25.755772293290153	26.525014139927432	27.301704587493997	20.41750897928842
22	24.409462503991207	23.342822850917244	26.907226682278935	25.340487962812613
23	22.91686219244106	27.40389939224382	25.372020373413264	24.307218041901855
24	23.164453687330575	24.234072780776252	31.13803201011421	21.463441521778964
25	23.72394293179912	24.309055363464413	28.54716032021039	23.419841384526077
26	20.993087201764226	27.05456000866024	27.930515477944446	24.02183731163109
27	22.5300315175134	24.905042752486466	28.09428538370971	24.470640346290423
28	22.22995221474217	25.676519179402536	30.739879461774027	21.353649144081267
29	22.831600540470482	24.942236099793373	29.791871192833852	22.434292166902292
30	24.408221070502993	25.233178452091344	28.38651882683542	21.972081650570242
31	27.876885551253576	24.575417332695736	24.427935034295842	23.11976208175485
32	28.402359518145044	25.248969486061434	24.453011990757776	21.89565900503575
33	28.071542322205623	24.214905047718222	24.894167795129707	22.81938483494645
34	23.34475948715886	26.54994212437078	27.040109722857427	23.065188665612936
35	24.689579556271944	24.68977818563006	28.065732413480777	22.554909844617217
36	27.679845228004158	26.309203342336207	23.93667497433957	22.074276455320064
37	25.349724227964927	28.1232852699944	25.094684132146128	21.432306369894544
38	26.210683180711502	28.122093493845718	23.595926310494434	22.07129701494835
39	24.394763931490747	27.094037593585462	24.145682716415376	24.365515758508412
40	26.208100999056015	23.80036557733361	26.95360663739863	23.037926786211745
41	22.52640653172781	24.29867697950294	26.8329889596837	26.341927529085545
42	25.03916722655317	25.87013314622448	24.798925017913888	24.291774609308465
43	23.894913151796032	24.377334205316213	26.969645958066362	24.758106684821392
44	23.6935029826681	26.080084377751326	27.354887598129107	22.871525041451466
45	21.74241645525054	26.742761573760266	27.272208127814018	24.24261384317517
46	24.1844650985872	26.281891805595485	26.643347580024045	22.89029551579327
47	23.676669144567906	25.528292020909717	27.213860753867934	23.581178080654443
48	24.299074238219166	24.251502506950786	27.448044767084735	24.001378487745313
49	24.38974854019836	23.211429530524615	27.49998634423163	24.898835585045394
50	22.188488336235803	25.686649276666362	28.031369534526995	24.093492852570837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1637.0
1	2153.5
2	2670.0
3	13149.0
4	23628.0
5	16167.5
6	8707.0
7	8955.0
8	9203.0
9	9560.0
10	9917.0
11	9868.0
12	9819.0
13	9734.5
14	9650.0
15	9245.5
16	8841.0
17	8342.5
18	7844.0
19	8135.0
20	8426.0
21	8536.5
22	8647.0
23	8928.0
24	9209.0
25	9985.5
26	10762.0
27	12540.0
28	14318.0
29	16298.0
30	18278.0
31	20828.5
32	23379.0
33	27671.5
34	31964.0
35	36451.5
36	40939.0
37	44956.0
38	48973.0
39	56320.0
40	63667.0
41	79133.0
42	94599.0
43	111603.5
44	128608.0
45	138215.5
46	147823.0
47	157642.5
48	167462.0
49	171770.5
50	176079.0
51	160703.5
52	145328.0
53	132341.5
54	119355.0
55	113197.5
56	107040.0
57	108206.5
58	109373.0
59	104043.0
60	98713.0
61	94418.5
62	90124.0
63	82347.0
64	74570.0
65	65057.0
66	55544.0
67	47266.5
68	38989.0
69	35589.5
70	32190.0
71	28001.5
72	23813.0
73	19396.5
74	14980.0
75	12202.5
76	9425.0
77	6934.5
78	4444.0
79	3520.5
80	2597.0
81	2005.0
82	1413.0
83	953.0
84	493.0
85	362.5
86	232.0
87	145.5
88	59.0
89	43.5
90	28.0
91	28.5
92	29.0
93	20.0
94	11.0
95	6.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2013801.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.541263651188224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78465793658314	19.089337984391776
2	6.578883945700905	3.2290825088403077
3	2.54154217927562	1.8711797010665543
4	1.507576889960006	1.4799136772378751
5	1.0276924720678808	1.2610435954679626
6	0.7581629747904037	1.1163766472940282
7	0.6316560051375981	1.085114559125667
8	0.5401301461175883	1.0604381057461243
9	0.46731851413925807	1.0321728178115759
>10	6.588412378754533	39.84550005253609
>50	1.47444991172587	23.64406693821422
>100	0.09422538318432601	3.4869421705929744
>500	0.004273712070066665	0.7730324557471335
>1k	8.140403942984123E-4	0.3096524890575157
>5k	0.0	0.0
>10k+	2.0351009857460308E-4	0.716146296870277
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14339	0.7120365915003518	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2052	0.10189686071265235	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04697584319403953	0.0
2	0.0	0.0	0.0	0.17752498881468426	0.0
3	0.0	0.0	0.0	0.32699358079571916	0.0
4	0.0	0.0	0.0	0.4958285351929014	0.0
5	0.0	0.0	0.0	0.7934746283272279	0.0
6	0.0	0.0	0.0	1.589084522254185	0.0
7	0.0	0.0	0.0	2.067334359253968	0.0
8	0.0	0.0	0.0	3.6039310736264407	0.0
9	0.0	0.0	0.0	4.496621066331778	0.0
10	0.0	0.0	0.0	5.697285878793386	0.0
11	0.0	0.0	0.0	6.278326408617336	0.0
12	0.0	0.0	0.0	6.804346606243616	0.0
13	4.965733952858301E-5	0.0	0.0	7.098020112215655	0.0
14	4.965733952858301E-5	0.0	0.0	7.244956179880733	0.0
15	4.965733952858301E-5	0.0	0.0	7.3522656906020005	0.0
16	4.965733952858301E-5	0.0	0.0	7.533068063825572	0.0
17	4.965733952858301E-5	0.0	0.0	7.715608443932643	0.0
18	4.965733952858301E-5	0.0	0.0	8.044141402253748	0.0
19	4.965733952858301E-5	0.0	0.0	8.17339945704665	0.0
20	4.965733952858301E-5	0.0	0.0	8.324357769213542	0.0
21	4.965733952858301E-5	0.0	0.0	8.478394836431207	0.0
22	4.965733952858301E-5	0.0	0.0	8.637099693564558	0.0
23	4.965733952858301E-5	0.0	0.0	8.817256521374256	0.0
24	4.965733952858301E-5	0.0	0.0	8.967469973448221	0.0
25	4.965733952858301E-5	0.0	0.0	9.088981483274663	0.0
26	4.965733952858301E-5	0.0	0.0	9.214316608244808	0.0
27	9.931467905716603E-5	0.0	0.0	9.364976976374528	0.0
28	9.931467905716603E-5	0.0	0.0	9.48544568207087	0.0
29	9.931467905716603E-5	0.0	0.0	9.625280750183359	0.0
30	9.931467905716603E-5	0.0	0.0	9.837863820705223	0.0
31	9.931467905716603E-5	0.0	0.0	9.983210853505387	0.0
32	9.931467905716603E-5	0.0	0.0	10.144994465689509	0.0
33	9.931467905716603E-5	0.0	0.0	10.298088043456131	0.0
34	9.931467905716603E-5	0.0	0.0	10.45406174691541	0.0
35	9.931467905716603E-5	0.0	0.0	10.634119260046052	0.0
36	9.931467905716603E-5	0.0	0.0	10.78036012495773	0.0
37	1.4897201858574905E-4	0.0	0.0	10.945321806871682	0.0
38	1.4897201858574905E-4	0.0	0.0	11.147228549394901	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAA	20	7.8578957E-4	44.0	26
ATCGCTA	25	4.4440363E-5	44.0	38
GTAATAG	265	0.0	41.509434	1
CGTTTTT	8020	0.0	41.25686	1
AACGTTG	95	0.0	39.36842	1
CGGTCTA	240	0.0	38.5	31
CACGACG	225	0.0	38.133335	26
TACGGGA	385	0.0	37.714287	4
CATACGA	210	0.0	37.714287	18
AGGGCGA	2255	0.0	37.365852	6
GGGCGAT	4440	0.0	37.36036	7
TAACGCC	190	0.0	37.05263	12
ATTAGCG	120	0.0	36.666664	1
TATTCGA	30	1.301443E-4	36.666664	27
TAATGCG	60	1.9826984E-10	36.666664	1
TCTATCG	30	1.301443E-4	36.666664	1
ATAGGGC	1225	0.0	36.636734	4
TTGATCC	1700	0.0	36.494118	17
ACGGGAT	350	0.0	36.45714	5
CATATGC	1615	0.0	36.371517	33
>>END_MODULE