FastQCFastQC Report
Thu 26 May 2016
SRR1546501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546501_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1673870
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174941.0451229784869793No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC18040.10777419990799764No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGC207.857512E-444.036
CGTTTTT101350.041.8293041
TAGCCGT1550.041.16129344
TCGATAG750.041.0666661
CATATGC18050.038.75900733
TTTAAGC14200.037.95774544
TAGTACG700.037.7142871
AAATGCG357.2921976E-637.7142871
CAGTAGC18950.037.38258727
ATATGCT18550.037.23988734
TAGCATA18750.037.19466830
GCCAGTA19150.037.1070525
GTTGATC19350.036.950916
AGGGCGA19600.036.8163266
GGTACCT19900.036.7035148
TCCGCAT301.3013493E-436.66666444
AGGGTAC11350.036.634366
TATGGGA8900.036.584274
TTGATCC19550.036.57288717
CCAGTAG19400.036.51546526
Produced by FastQC (version 0.11.5)