FastQCFastQC Report
Thu 26 May 2016
SRR1546500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546500_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2332403
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259301.1117289765104916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT155100.042.510641
ACGGGTA1900.040.5263145
CGAATAT1500.039.614
CGTTCGA2200.039.014
GGGCGAT49800.037.682737
ACGGGAT7250.037.020695
GTTTTTT185100.036.928152
ACCCGTA301.3015061E-436.66666434
TGGGCGA15950.036.5517236
TACGGGA6600.036.3333324
CGTTGAT1400.036.14285725
TTAACGG1400.036.1428572
AGGGCGA25950.036.1156046
GGCGATA10300.036.0970888
GGCGATT14100.036.0425538
AACGGGA6950.035.7697874
TAACGCC1750.035.212
GACCGAT13800.035.0724649
ATAGGGC13250.034.8679244
CGGGATC4550.034.8131876
Produced by FastQC (version 0.11.5)