##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546500_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2332403
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44916165859845	31.0	31.0	33.0	30.0	34.0
2	31.836743907463674	31.0	31.0	34.0	30.0	34.0
3	31.94667945462255	33.0	31.0	34.0	30.0	34.0
4	35.64524012359785	37.0	35.0	37.0	33.0	37.0
5	35.54789245254786	37.0	35.0	37.0	33.0	37.0
6	35.576248615698056	37.0	35.0	37.0	33.0	37.0
7	36.012294187582505	37.0	35.0	37.0	35.0	37.0
8	35.99386812656304	37.0	35.0	37.0	35.0	37.0
9	37.68548102536311	39.0	37.0	39.0	35.0	39.0
10	37.11573600274052	39.0	37.0	39.0	33.0	39.0
11	36.920470004540384	39.0	37.0	39.0	32.0	39.0
12	36.6495318347644	39.0	35.0	39.0	32.0	39.0
13	36.5819341683234	39.0	35.0	39.0	32.0	39.0
14	37.70995578379894	40.0	36.0	41.0	33.0	41.0
15	37.80683483943384	40.0	36.0	41.0	33.0	41.0
16	37.81691285768368	40.0	36.0	41.0	33.0	41.0
17	37.74176589551634	40.0	36.0	41.0	33.0	41.0
18	37.64542233910692	39.0	36.0	41.0	33.0	41.0
19	37.570713551646094	39.0	36.0	41.0	33.0	41.0
20	37.38588700151732	39.0	35.0	41.0	32.0	41.0
21	37.29404309632598	39.0	35.0	41.0	32.0	41.0
22	37.22828259095877	39.0	35.0	41.0	32.0	41.0
23	37.12271507110906	39.0	35.0	41.0	32.0	41.0
24	37.056937416046885	39.0	35.0	41.0	32.0	41.0
25	36.871829610920585	39.0	35.0	41.0	31.0	41.0
26	36.790931927287005	38.0	35.0	41.0	31.0	41.0
27	36.70269889037186	38.0	35.0	41.0	31.0	41.0
28	36.722100769035194	38.0	35.0	41.0	31.0	41.0
29	36.63563243573259	38.0	35.0	41.0	31.0	41.0
30	36.583707875525796	38.0	35.0	41.0	31.0	41.0
31	36.44990509787545	38.0	35.0	40.0	31.0	41.0
32	36.20231195037907	38.0	35.0	41.0	30.0	41.0
33	36.03647697246145	38.0	35.0	41.0	29.0	41.0
34	35.8406943397003	39.0	35.0	41.0	28.0	41.0
35	35.606355762704816	38.0	35.0	41.0	27.0	41.0
36	35.45083032391915	38.0	35.0	41.0	26.0	41.0
37	35.40605461406112	38.0	35.0	41.0	26.0	41.0
38	35.36560105607822	38.0	35.0	40.0	26.0	41.0
39	35.24913833501329	38.0	35.0	40.0	25.0	41.0
40	35.152121224333875	38.0	34.0	40.0	25.0	41.0
41	35.111922768063664	38.0	34.0	40.0	24.0	41.0
42	34.928624684499205	38.0	34.0	40.0	24.0	41.0
43	34.939134446319954	38.0	34.0	40.0	24.0	41.0
44	34.60712578400902	38.0	34.0	40.0	23.0	41.0
45	34.55015621228407	37.0	34.0	40.0	23.0	41.0
46	34.56196849343788	37.0	34.0	40.0	23.0	41.0
47	34.48962036148985	37.0	34.0	40.0	23.0	41.0
48	34.445628821434376	37.0	34.0	40.0	23.0	41.0
49	34.424111527896336	37.0	34.0	40.0	24.0	41.0
50	33.90704951074064	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	8.0
13	12.0
14	24.0
15	65.0
16	136.0
17	357.0
18	820.0
19	1820.0
20	3052.0
21	5174.0
22	7850.0
23	11470.0
24	16725.0
25	24938.0
26	36886.0
27	45577.0
28	48221.0
29	49906.0
30	54881.0
31	64895.0
32	79995.0
33	101640.0
34	156261.0
35	226080.0
36	184801.0
37	234174.0
38	344902.0
39	631730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.63368037170249	22.646858197318387	25.894152940122268	16.825308490856855
2	35.8696588882796	24.261759224284997	26.367656018278147	13.50092586915726
3	29.32769337031379	24.493794597245845	32.267322585333666	13.911189447106697
4	25.43844267049905	27.509096841326308	31.96664555825044	15.0858149299242
5	22.7997906022244	30.808569531080177	31.0440777172727	15.34756214942272
6	20.177430744172426	39.714706249305976	29.536405158113755	10.571457848407844
7	82.80215726013043	4.222512147343319	10.580933054879454	2.394397537646796
8	82.03869571424835	3.9351690081002295	10.543375222892442	3.482760054758976
9	75.71067264104873	5.874199270023234	13.67911119990842	4.7360168890196075
10	37.32155206454459	28.89770764314743	19.411525366756948	14.369214925551029
11	29.251677347353784	25.44350183051557	29.27757338676035	16.027247435370302
12	26.914559790910918	22.38369612798474	32.035930325934245	18.665813755170095
13	22.756744867846596	26.12404460121171	33.19872251922159	17.9204880117201
14	19.63477152104503	28.967335404730658	32.50038693999279	18.89750613423152
15	18.68309207285362	23.882022103384365	39.236487005033005	18.19839881872901
16	21.019052024885923	22.982220482480944	36.77542002818552	19.22330746444761
17	20.90346308077978	22.636482631860787	32.379481590445565	24.08057269691387
18	23.1249916931165	22.6612210668568	33.896243487939266	20.31754375208744
19	25.565607658710782	25.348535394612338	29.490786969490262	19.59506997718662
20	26.28971065463387	24.05377629852131	29.12421223948006	20.532300807364763
21	24.082201917936136	26.298799992968625	30.291763473121925	19.327234615973314
22	23.136481988747228	23.89081132205712	29.62318261466822	23.34952407452743
23	21.096139903781637	27.420733037987006	28.922531826618297	22.56059523161306
24	21.122764805224485	24.47767388397288	33.28086955813382	21.118691752668813
25	21.460313676495872	25.424036926723215	31.347798815213324	21.767850581567593
26	20.004433196150064	26.86701226160316	30.89761932221833	22.230935220028442
27	21.0145931041934	25.455892485132285	30.62918372168103	22.900330688993282
28	20.670398726120656	25.887507433320916	33.554964558011626	19.8871292825468
29	21.45877020394846	23.97771740132387	32.395473681006244	22.168038713721426
30	21.83722109772625	24.97522940932592	32.35187058154187	20.83567891140596
31	24.616414916290193	25.237019503061862	28.496490529295322	21.65007505135262
32	25.443458956278135	25.3234539657169	29.111349968251627	20.121737109753333
33	24.401271992876016	25.87318743801993	28.090771620513266	21.63476894859079
34	22.727461763683205	26.435182942227392	29.532760847932373	21.304594446157033
35	23.358184670487905	26.718495903152245	29.05977226062563	20.86354716573422
36	26.09416125772433	26.449117069391527	26.50991273806456	20.946808934819583
37	23.24126662502149	29.01544029912498	27.325509356659204	20.417783719194325
38	23.611614287925374	28.339013455221934	26.566978348081356	21.482393908771343
39	22.55549319735912	28.383645536384577	26.939083854719787	22.121777411536513
40	24.062951385330923	25.96682477256289	28.59437241334366	21.375851428762523
41	20.944493725998466	26.24859426094033	28.822206111036557	23.98470590202465
42	23.27325080614285	26.64608131613619	27.474540205959265	22.606127671761698
43	22.44457754513264	25.966010162051756	28.787735224144367	22.801677068671236
44	22.61937581112698	27.182438026361655	28.761967807450084	21.436218355061282
45	21.09896960345189	27.396852087739553	29.094972009554095	22.40920629925446
46	23.014762028688867	27.032678315025322	28.28010425299573	21.672455403290083
47	22.24606982584056	26.4733410135384	28.90945518420273	22.37113397641831
48	22.864316329553684	25.14548300615288	29.422188189605315	22.568012474688125
49	22.767377678728764	24.957951091642396	29.030875024599094	23.24379620502975
50	21.134083603905502	26.367055778954153	29.896505878272322	22.602354738868023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1926.0
1	2569.0
2	3212.0
3	22898.5
4	42585.0
5	29440.0
6	16295.0
7	16301.0
8	16307.0
9	16612.0
10	16917.0
11	16705.5
12	16494.0
13	15930.5
14	15367.0
15	14565.5
16	13764.0
17	13029.0
18	12294.0
19	12034.0
20	11774.0
21	12201.5
22	12629.0
23	13547.5
24	14466.0
25	15759.5
26	17053.0
27	20156.5
28	23260.0
29	27008.5
30	30757.0
31	36249.0
32	41741.0
33	46572.0
34	51403.0
35	59655.5
36	67908.0
37	73548.5
38	79189.0
39	87681.0
40	96173.0
41	111194.0
42	126215.0
43	139747.0
44	153279.0
45	164734.0
46	176189.0
47	185874.5
48	195560.0
49	199379.5
50	203199.0
51	184488.0
52	165777.0
53	149916.0
54	134055.0
55	121344.5
56	108634.0
57	102662.5
58	96691.0
59	91895.0
60	87099.0
61	81622.0
62	76145.0
63	68086.5
64	60028.0
65	52643.0
66	45258.0
67	39180.5
68	33103.0
69	29357.0
70	25611.0
71	21624.5
72	17638.0
73	14353.5
74	11069.0
75	9112.0
76	7155.0
77	5801.0
78	4447.0
79	3286.0
80	2125.0
81	1577.0
82	1029.0
83	677.0
84	325.0
85	245.5
86	166.0
87	106.0
88	46.0
89	32.5
90	19.0
91	15.5
92	12.0
93	11.5
94	11.0
95	7.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2332403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.186199681554058
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.27869064244757	20.236352242921747
2	6.434949014193581	3.3701371965258504
3	2.474640345881612	1.9440427871185766
4	1.5350130463159326	1.6078463257847837
5	1.0354591055702216	1.3557369450272592
6	0.8207761815650363	1.2895805390595305
7	0.6678557023587715	1.2242021946301924
8	0.5816334230532182	1.2184615166029902
9	0.49868720601035477	1.175285047970109
>10	7.831841980866942	49.954044072082596
>50	0.7909132249738848	12.716714930606496
>100	0.04623766774559576	2.006661332549359
>500	0.0018163524595066702	0.31919344002564803
>1k	0.001320983606913942	0.46054312242118356
>5k	0.0	0.0
>10k+	1.6512295086424275E-4	1.1211983066736728
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25930	1.1117289765104916	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05440740729625198	0.0
2	0.0	0.0	0.0	0.19782173149322824	0.0
3	0.0	0.0	0.0	0.3898983151710918	0.0
4	0.0	0.0	0.0	0.5559502367301019	0.0
5	0.0	0.0	0.0	0.8570131319501818	0.0
6	0.0	0.0	0.0	1.5836885821189564	0.0
7	0.0	0.0	0.0	2.0264079578014607	0.0
8	0.0	0.0	0.0	3.328369925780408	0.0
9	0.0	0.0	0.0	4.109195537820866	0.0
10	0.0	0.0	0.0	5.157342020225493	0.0
11	0.0	0.0	0.0	5.66750257138239	0.0
12	4.287423742809454E-5	0.0	0.0	6.172389591335631	0.0
13	4.287423742809454E-5	0.0	0.0	6.472852247231717	0.0
14	4.287423742809454E-5	0.0	0.0	6.625698903662875	0.0
15	4.287423742809454E-5	0.0	0.0	6.740301740308172	0.0
16	4.287423742809454E-5	0.0	0.0	6.929677247028065	0.0
17	4.287423742809454E-5	0.0	0.0	7.12128221409422	0.0
18	4.287423742809454E-5	0.0	0.0	7.453514679924524	0.0
19	4.287423742809454E-5	0.0	0.0	7.591955592579842	0.0
20	4.287423742809454E-5	0.0	0.0	7.754234581245179	0.0
21	4.287423742809454E-5	0.0	0.0	7.91689943804737	0.0
22	4.287423742809454E-5	0.0	0.0	8.079307049424992	0.0
23	4.287423742809454E-5	0.0	0.0	8.263666270365798	0.0
24	4.287423742809454E-5	0.0	0.0	8.410853527456448	0.0
25	4.287423742809454E-5	0.0	0.0	8.543720789246112	0.0
26	4.287423742809454E-5	0.0	0.0	8.675730566287216	0.0
27	4.287423742809454E-5	0.0	0.0	8.847827755323587	0.0
28	4.287423742809454E-5	4.287423742809454E-5	0.0	8.978165437104995	0.0
29	4.287423742809454E-5	4.287423742809454E-5	0.0	9.119736169092562	0.0
30	4.287423742809454E-5	4.287423742809454E-5	0.0	9.3346647213196	0.0
31	4.287423742809454E-5	4.287423742809454E-5	0.0	9.486268024865343	0.0
32	4.287423742809454E-5	4.287423742809454E-5	0.0	9.653906293209193	0.0
33	4.287423742809454E-5	4.287423742809454E-5	0.0	9.810054265922313	0.0
34	4.287423742809454E-5	4.287423742809454E-5	0.0	9.967316968808564	0.0
35	4.287423742809454E-5	4.287423742809454E-5	0.0	10.133926255454138	0.0
36	8.574847485618909E-5	4.287423742809454E-5	0.0	10.286129798323875	0.0
37	8.574847485618909E-5	4.287423742809454E-5	0.0	10.444592979858111	0.0
38	8.574847485618909E-5	4.287423742809454E-5	0.0	10.619134000427884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15510	0.0	42.51064	1
ACGGGTA	190	0.0	40.526314	5
CGAATAT	150	0.0	39.6	14
CGTTCGA	220	0.0	39.0	14
GGGCGAT	4980	0.0	37.68273	7
ACGGGAT	725	0.0	37.02069	5
GTTTTTT	18510	0.0	36.92815	2
ACCCGTA	30	1.3015061E-4	36.666664	34
TGGGCGA	1595	0.0	36.551723	6
TACGGGA	660	0.0	36.333332	4
CGTTGAT	140	0.0	36.142857	25
TTAACGG	140	0.0	36.142857	2
AGGGCGA	2595	0.0	36.115604	6
GGCGATA	1030	0.0	36.097088	8
GGCGATT	1410	0.0	36.042553	8
AACGGGA	695	0.0	35.769787	4
TAACGCC	175	0.0	35.2	12
GACCGAT	1380	0.0	35.072464	9
ATAGGGC	1325	0.0	34.867924	4
CGGGATC	455	0.0	34.813187	6
>>END_MODULE