FastQCFastQC Report
Thu 26 May 2016
SRR1546499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546499_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2137720
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133430.6241696761035121No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25530.11942630466104072No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC23680.11077222461313924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG850.044.01
CGTTTTT75600.041.6428571
ACGGGTA2650.041.5094385
ATGGTCG2850.040.9122844
TGCGATT404.1267594E-738.51
GGCGATA9900.038.4444438
CGCATCG3100.038.32258221
AGGGTAC15650.038.236426
CATATGC24650.038.19878433
TACGGGT1850.038.0540544
TAGCCGT4400.037.544
TCGTAAG1000.037.3999981
ATATGCT25100.037.33864634
GGGCGAT39850.037.2095387
GTCTCAA24900.037.10843342
TAGCATA25350.037.057230
AGGGCGA21700.037.004616
TCTACGG1250.036.962
ATAGGGC13700.036.9343074
TACGGGA4000.036.854
Produced by FastQC (version 0.11.5)