FastQCFastQC Report
Thu 26 May 2016
SRR1546498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546498_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1460130
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193641.3261832850499613No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC15040.10300452699417174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110300.042.5639151
GCGAACT452.3517714E-839.1111133
TACGTAG1300.038.9230771
CCGGTAT404.125559E-738.542
AGGGTAC6600.038.3333326
CGGTCTA750.038.1333331
ACGTAGG2250.038.133332
GTTGATC11350.037.99118816
TAGCATA11100.037.85585830
GTTTTTT127200.037.7562872
ATAACGC1350.037.48148311
CGCCGTT1000.037.39999826
GTAGCAT11250.037.3511129
TATGGGC7550.037.0066224
CATATGC11200.036.73214333
ATTTACG301.3012678E-436.6666641
GCCAGTA11350.036.6343625
GCGCGAC10200.036.450989
CGTTAGG2300.036.3478282
ATCCTGC11750.036.32340220
Produced by FastQC (version 0.11.5)