##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546498_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1460130
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.447193058152358	31.0	31.0	33.0	30.0	34.0
2	31.846999239793718	31.0	31.0	34.0	30.0	34.0
3	31.954795805852903	33.0	31.0	34.0	30.0	34.0
4	35.66242594837446	37.0	35.0	37.0	33.0	37.0
5	35.5656468944546	37.0	35.0	37.0	33.0	37.0
6	35.59357865395547	37.0	35.0	37.0	33.0	37.0
7	36.01507468513078	37.0	35.0	37.0	35.0	37.0
8	36.00621040592276	37.0	35.0	37.0	35.0	37.0
9	37.70432153301418	39.0	37.0	39.0	35.0	39.0
10	37.124151274201616	39.0	37.0	39.0	33.0	39.0
11	36.91700122591824	39.0	37.0	39.0	32.0	39.0
12	36.60350585221864	39.0	35.0	39.0	32.0	39.0
13	36.529850766712556	39.0	35.0	39.0	32.0	39.0
14	37.60627135939951	40.0	36.0	41.0	32.0	41.0
15	37.700840336134455	40.0	36.0	41.0	33.0	41.0
16	37.72823036304987	40.0	36.0	41.0	33.0	41.0
17	37.642564703142874	40.0	36.0	41.0	32.0	41.0
18	37.522158985843724	39.0	36.0	41.0	32.0	41.0
19	37.42668050105128	39.0	36.0	41.0	32.0	41.0
20	37.21515070575908	39.0	35.0	41.0	32.0	41.0
21	37.120190667954226	39.0	35.0	41.0	32.0	41.0
22	37.0476964379884	39.0	35.0	41.0	32.0	41.0
23	36.941228520748155	39.0	35.0	41.0	32.0	41.0
24	36.87081492743797	38.0	35.0	41.0	32.0	41.0
25	36.68993240327916	38.0	35.0	41.0	31.0	41.0
26	36.596126372309314	38.0	35.0	41.0	31.0	41.0
27	36.505976180203135	38.0	35.0	40.0	31.0	41.0
28	36.53998753535644	38.0	35.0	40.0	31.0	41.0
29	36.41760117249834	38.0	35.0	40.0	31.0	41.0
30	36.32898098114551	38.0	35.0	40.0	30.0	41.0
31	36.17005198167286	38.0	35.0	40.0	30.0	41.0
32	35.881033195674355	38.0	35.0	40.0	29.0	41.0
33	35.6566826241499	38.0	35.0	40.0	27.0	41.0
34	35.390309082068036	38.0	35.0	40.0	26.0	41.0
35	35.11511577736229	38.0	34.0	40.0	24.0	41.0
36	34.97025333360728	38.0	34.0	40.0	24.0	41.0
37	34.903948278577936	38.0	34.0	40.0	23.0	41.0
38	34.82146110277852	38.0	34.0	40.0	23.0	41.0
39	34.685281447542344	38.0	34.0	40.0	23.0	41.0
40	34.56042201721765	37.0	34.0	40.0	23.0	41.0
41	34.50432906658996	37.0	34.0	40.0	23.0	41.0
42	34.320806366556404	37.0	33.0	40.0	22.0	41.0
43	34.345085026675704	37.0	33.0	40.0	23.0	41.0
44	33.98448357338045	37.0	33.0	40.0	21.0	41.0
45	33.919122954805395	36.0	33.0	40.0	21.0	41.0
46	33.913378260839785	36.0	33.0	40.0	22.0	41.0
47	33.82813790552896	36.0	33.0	40.0	22.0	41.0
48	33.77339962880018	36.0	33.0	40.0	22.0	41.0
49	33.73671316937533	36.0	33.0	40.0	22.0	41.0
50	33.25563134789368	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	7.0
14	8.0
15	41.0
16	112.0
17	293.0
18	611.0
19	1268.0
20	2149.0
21	3613.0
22	5408.0
23	7942.0
24	11799.0
25	17918.0
26	26397.0
27	32925.0
28	33858.0
29	34485.0
30	37069.0
31	43209.0
32	52244.0
33	66462.0
34	104366.0
35	143093.0
36	119358.0
37	151324.0
38	216257.0
39	347908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.25820303671591	22.038654092443824	25.877284899289787	18.825857971550477
2	36.49017553231562	24.01669714340504	26.31334196270195	13.179785361577393
3	28.9498195366166	24.504256470314285	33.35127694109429	13.194647051974826
4	25.62908782094745	26.87671645675385	32.63229986371077	14.861895858587934
5	22.505872764753825	30.37428174203667	32.01666974858403	15.103175744625478
6	20.473177045879474	38.58964612739961	30.215460267236477	10.72171655948443
7	82.36026929109052	4.038886948422401	11.460554882099538	2.140288878387541
8	81.99098710388802	3.70330039106107	11.420079034058611	2.885633470992309
9	76.68118592180149	5.245286378610124	13.977043139994382	4.096484559594009
10	38.92482176244581	25.496359913158418	20.47351948114209	15.105298843253683
11	31.2949531890996	25.342880428455	27.03204509187538	16.33012129057002
12	28.33117599117887	21.268585673878356	31.658619437995245	18.741618896947532
13	24.60000136974105	24.56733304568771	32.16446480792806	18.668200776643175
14	20.955873792059613	27.27907789032483	32.70866292727359	19.05638539034196
15	20.439138980775684	22.248977830741097	38.32508064350434	18.98680254497887
16	23.02007355509441	21.306116578660806	35.44917233397026	20.22463753227452
17	22.240074513913143	20.952997335853656	32.513543314636365	24.29338483559683
18	24.211063398464518	21.706354913603583	33.251696766726255	20.830884921205648
19	27.12094128604987	24.30495914747317	28.732441631909488	19.84165793456747
20	27.932786806654203	24.01614924698486	28.193996424975857	19.857067521385083
21	25.79010088142837	25.500811571572395	30.014313793977248	18.69477375302199
22	24.598426167533027	22.470533445652098	29.87117585420476	23.05986453261011
23	22.59339921787786	25.73209234794162	29.223493798497397	22.451014635683126
24	22.6613383739804	23.821029634347628	32.624149904460566	20.893482087211414
25	23.706450795477117	23.690561799291842	30.752946655434787	21.85004074979625
26	21.11394190928205	25.2527514673351	30.072116866306427	23.561189757076427
27	21.71834014779506	24.04148945641826	30.73281146199311	23.507358933793565
28	21.54267085807428	25.41506578181395	32.02687431940991	21.015389040701855
29	22.810777122584973	24.030120605699494	31.43261216467027	21.72649010704526
30	23.42866731044496	23.88739358824214	31.896201023196564	20.787738078116334
31	25.65326375048797	24.352694623081504	28.637792525323086	21.356249101107437
32	26.151849492853373	23.900200667063892	28.934546923904037	21.0134029161787
33	25.91406244649449	24.69492442453754	27.475224808749903	21.915788320218063
34	22.987679179251163	25.838315766404364	30.02739482100909	21.146610233335387
35	23.140473793429354	25.61128118729154	29.943840616931368	21.30440440234774
36	26.20965256518255	26.482299521275504	26.378336175546014	20.92971173799593
37	24.668899344578907	27.04211268859622	27.36982323491744	20.919164731907433
38	24.578427948196392	27.299487031976604	27.041907227438656	21.080177792388348
39	24.291193249916105	25.340003972249047	27.068137768554855	23.300665009279996
40	24.912028381034563	24.014094635409176	28.967078273852326	22.10679870970393
41	22.289933088149684	24.19620170806709	29.122886318341518	24.390978885441708
42	23.656386760083006	25.953237040537484	26.818844897372152	23.571531302007358
43	22.64072377117106	24.90908343777609	28.4060323395862	24.044160451466652
44	23.26443535849548	25.65956455932006	28.404868059693317	22.671132022491147
45	22.40560771986056	26.146439015704082	28.31919075698739	23.128762507447966
46	23.685219809195072	25.874887852451494	28.107154842377046	22.332737495976385
47	22.34492819132543	26.217254628012576	28.861265777704727	22.57655140295727
48	23.143144788477738	25.295418901056756	28.84181545478827	22.719620855677235
49	23.883489826248347	24.18092909535452	28.907152102894944	23.028428975502184
50	21.96407169224658	25.217138200023285	29.536890550841367	23.28189955688877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1154.0
1	1659.0
2	2164.0
3	17228.5
4	32293.0
5	22142.5
6	11992.0
7	11917.5
8	11843.0
9	12060.5
10	12278.0
11	11982.0
12	11686.0
13	11315.0
14	10944.0
15	10467.5
16	9991.0
17	9299.0
18	8607.0
19	8193.0
20	7779.0
21	7699.0
22	7619.0
23	7969.0
24	8319.0
25	8890.0
26	9461.0
27	10394.0
28	11327.0
29	13444.0
30	15561.0
31	18128.5
32	20696.0
33	22850.0
34	25004.0
35	29429.5
36	33855.0
37	37209.0
38	40563.0
39	45254.0
40	49945.0
41	57263.5
42	64582.0
43	72395.5
44	80209.0
45	87418.5
46	94628.0
47	101335.5
48	108043.0
49	112759.5
50	117476.0
51	108284.5
52	99093.0
53	91961.5
54	84830.0
55	81866.5
56	78903.0
57	78187.5
58	77472.0
59	75437.5
60	73403.0
61	67809.0
62	62215.0
63	57623.0
64	53031.0
65	44789.0
66	36547.0
67	32033.5
68	27520.0
69	24188.0
70	20856.0
71	18220.5
72	15585.0
73	13097.5
74	10610.0
75	8315.0
76	6020.0
77	4421.0
78	2822.0
79	2342.5
80	1863.0
81	1321.5
82	780.0
83	541.5
84	303.0
85	243.0
86	183.0
87	108.0
88	33.0
89	24.5
90	16.0
91	16.0
92	16.0
93	11.5
94	7.0
95	5.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1460130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.989873698845322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70799609097	23.2591961184627
2	6.852915834289586	4.384478240154821
3	3.296445178564759	3.1635859475236274
4	2.255386664980518	2.8859813821914697
5	1.69916268738908	2.7177999881683634
6	1.408770710603906	2.703983826171092
7	1.233489271849889	2.762141621075105
8	1.0641944859763421	2.723475775791264
9	0.9962883360864738	2.8684024235135155
>10	8.369283276436002	46.861525014352885
>50	0.08508486365733567	1.749447429935715
>100	0.026894622371062354	1.6029447058255406
>500	0.0032273501249953066	0.7149200567735327
>1k	6.454700249990613E-4	0.26932532802106796
>5k	0.0	0.0
>10k+	2.1515667499968707E-4	1.3327921420393458
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19364	1.3261832850499613	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	1504	0.10300452699417174	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.053419900967722055	0.0
2	0.0	0.0	0.0	0.19244861758884482	0.0
3	0.0	0.0	0.0	0.3749666125618952	0.0
4	0.0	0.0	0.0	0.5095436707690412	0.0
5	0.0	0.0	0.0	0.7998602864128537	0.0
6	0.0	0.0	0.0	1.4144630957517481	0.0
7	0.0	0.0	0.0	1.7727188674980996	0.0
8	0.0	0.0	0.0	2.6369569832823103	0.0
9	0.0	0.0	0.0	3.1962907412353694	0.0
10	0.0	0.0	0.0	3.981700259565929	0.0
11	0.0	0.0	0.0	4.413442638669159	0.0
12	0.0	0.0	0.0	4.8077910870949845	0.0
13	0.0	0.0	0.0	5.072425058042777	0.0
14	0.0	0.0	0.0	5.211042852348763	0.0
15	0.0	0.0	0.0	5.3152801462883446	0.0
16	0.0	0.0	0.0	5.470677268462397	0.0
17	0.0	0.0	0.0	5.621554245169951	0.0
18	0.0	0.0	0.0	5.9096107880805135	0.0
19	0.0	0.0	0.0	6.017477895803799	0.0
20	0.0	0.0	0.0	6.155547793689603	0.0
21	0.0	0.0	0.0	6.285810167587818	0.0
22	0.0	0.0	0.0	6.415867080328464	0.0
23	0.0	0.0	0.0	6.56934656503188	0.0
24	0.0	0.0	0.0	6.693034181887914	0.0
25	0.0	0.0	0.0	6.810626451069425	0.0
26	0.0	0.0	0.0	6.9215754761562325	0.0
27	0.0	0.0	0.0	7.0653297994014235	0.0
28	0.0	0.0	0.0	7.178812845431571	0.0
29	0.0	0.0	0.0	7.308595809962127	0.0
30	0.0	0.0	0.0	7.496250333874381	0.0
31	0.0	0.0	0.0	7.630690417976481	0.0
32	0.0	0.0	0.0	7.784375363837467	0.0
33	0.0	0.0	0.0	7.9313485785512245	0.0
34	0.0	0.0	0.0	8.07674659105696	0.0
35	0.0	0.0	0.0	8.246594481313307	0.0
36	0.0	0.0	0.0	8.393704670132111	0.0
37	0.0	0.0	0.0	8.551361865039414	0.0
38	0.0	0.0	0.0	8.740728565264737	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11030	0.0	42.563915	1
GCGAACT	45	2.3517714E-8	39.11111	33
TACGTAG	130	0.0	38.923077	1
CCGGTAT	40	4.125559E-7	38.5	42
AGGGTAC	660	0.0	38.333332	6
CGGTCTA	75	0.0	38.13333	31
ACGTAGG	225	0.0	38.13333	2
GTTGATC	1135	0.0	37.991188	16
TAGCATA	1110	0.0	37.855858	30
GTTTTTT	12720	0.0	37.756287	2
ATAACGC	135	0.0	37.481483	11
CGCCGTT	100	0.0	37.399998	26
GTAGCAT	1125	0.0	37.35111	29
TATGGGC	755	0.0	37.006622	4
CATATGC	1120	0.0	36.732143	33
ATTTACG	30	1.3012678E-4	36.666664	1
GCCAGTA	1135	0.0	36.63436	25
GCGCGAC	1020	0.0	36.45098	9
CGTTAGG	230	0.0	36.347828	2
ATCCTGC	1175	0.0	36.323402	20
>>END_MODULE