FastQCFastQC Report
Thu 26 May 2016
SRR1546497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546497_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1100242
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60270.5477885774220581No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17890.16260059150623227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG302.5276186E-644.01
GTCGACG254.442715E-544.012
ACGTTAG502.7284841E-1144.01
CACGACG302.5276186E-644.026
TATTCCG207.856334E-444.01
GCGATCG351.4460056E-744.09
TCGTAAG351.4460056E-744.01
CCGCATA207.856334E-444.019
AGTCGAC254.442715E-544.011
CGAAGTA207.856334E-444.038
TATAGCG1000.041.8000031
CGTTTTT39750.041.6754721
AGTACGG1650.041.3333322
TAGTACG700.040.8571431
AGGGCGA10650.039.45546
TACGGGA4750.039.368424
CGTTAGG1900.039.368422
GCGATAC900.039.111119
GAGTACG850.038.8235321
ACGGGCC1550.038.3225825
Produced by FastQC (version 0.11.5)