##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546497_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1100242
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420070311804132	31.0	31.0	33.0	30.0	34.0
2	31.801251906398775	31.0	31.0	34.0	30.0	34.0
3	31.934314450820818	33.0	31.0	34.0	30.0	34.0
4	35.60884605386815	37.0	35.0	37.0	33.0	37.0
5	35.49039302262593	37.0	35.0	37.0	33.0	37.0
6	35.52644963562562	37.0	35.0	37.0	33.0	37.0
7	36.01570109121448	37.0	35.0	37.0	35.0	37.0
8	36.007679219662585	37.0	35.0	37.0	35.0	37.0
9	37.69526522346902	39.0	37.0	39.0	35.0	39.0
10	37.05167499513743	39.0	37.0	39.0	33.0	39.0
11	36.81499797317318	39.0	37.0	39.0	32.0	39.0
12	36.28763581103066	38.0	35.0	39.0	32.0	39.0
13	36.050177142846756	38.0	35.0	39.0	31.0	39.0
14	37.07333931989508	39.0	35.0	41.0	31.0	41.0
15	37.291069601051404	39.0	35.0	41.0	32.0	41.0
16	37.3846635558359	39.0	35.0	41.0	32.0	41.0
17	37.32712894072395	39.0	35.0	41.0	32.0	41.0
18	37.27946124579865	39.0	35.0	41.0	32.0	41.0
19	37.25441039334983	39.0	35.0	41.0	32.0	41.0
20	37.102222056602095	39.0	35.0	41.0	32.0	41.0
21	36.978347490824746	39.0	35.0	41.0	32.0	41.0
22	36.90771939264271	38.0	35.0	41.0	32.0	41.0
23	36.8198287285888	38.0	35.0	41.0	31.0	41.0
24	36.77041414525168	38.0	35.0	41.0	31.0	41.0
25	36.60915598568315	38.0	35.0	41.0	31.0	41.0
26	36.51998742094921	38.0	35.0	41.0	31.0	41.0
27	36.41800258488587	38.0	35.0	40.0	31.0	41.0
28	36.47692689426508	38.0	35.0	40.0	31.0	41.0
29	36.44968288794647	38.0	35.0	40.0	31.0	41.0
30	36.43369549608177	38.0	35.0	40.0	31.0	41.0
31	36.308915674915156	38.0	35.0	40.0	30.0	41.0
32	36.0991863608188	38.0	35.0	40.0	30.0	41.0
33	36.01911215896139	38.0	35.0	41.0	30.0	41.0
34	35.94170918761509	38.0	35.0	41.0	30.0	41.0
35	35.779464881362465	38.0	35.0	41.0	29.0	41.0
36	35.63617731371826	38.0	35.0	40.0	28.0	41.0
37	35.60275284891869	38.0	35.0	40.0	28.0	41.0
38	35.572070508124575	38.0	35.0	40.0	28.0	41.0
39	35.475156374688474	38.0	35.0	40.0	27.0	41.0
40	35.36798358906495	38.0	34.0	40.0	27.0	41.0
41	35.36838440997526	38.0	34.0	40.0	27.0	41.0
42	35.19252037279071	38.0	34.0	40.0	27.0	41.0
43	35.21968530559641	38.0	34.0	40.0	27.0	41.0
44	34.88271762030535	37.0	34.0	40.0	26.0	41.0
45	34.836225121382384	37.0	34.0	40.0	25.0	41.0
46	34.8773351680812	37.0	34.0	40.0	26.0	41.0
47	34.804159448557684	37.0	34.0	40.0	26.0	41.0
48	34.786237936744826	37.0	34.0	40.0	26.0	41.0
49	34.79274559596889	37.0	34.0	40.0	26.0	41.0
50	34.2301111937192	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	5.0
14	3.0
15	28.0
16	77.0
17	174.0
18	463.0
19	864.0
20	1541.0
21	2526.0
22	3690.0
23	5482.0
24	7607.0
25	11152.0
26	14962.0
27	18369.0
28	20477.0
29	22644.0
30	26343.0
31	32074.0
32	40047.0
33	52162.0
34	84934.0
35	135849.0
36	78731.0
37	98623.0
38	148888.0
39	292520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.4607822642655	24.106787415859422	27.931582324615857	13.500847995259225
2	31.89271087633448	25.76596785070921	28.468100654219707	13.873220618736607
3	29.263925572737637	25.810957952886724	31.255305650938613	13.669810823437025
4	25.646903135855563	28.611069201139383	30.7662314290856	14.975796233919446
5	23.40230603812616	32.109208701358426	29.530321511085745	14.958163749429671
6	20.820783064089536	41.23383764662683	27.147209432106756	10.798169857176877
7	86.4065360166218	4.288147516637249	7.3006665806249895	2.0046498861159634
8	86.14350297480009	3.785530819583328	6.939109759489276	3.1318564461273066
9	79.8509782393328	6.107292759229333	9.963898851343615	4.077830150094252
10	41.042425211907926	29.849433124712565	15.906682348065242	13.201459315314267
11	35.611983545438186	24.290474277477138	25.856402500540792	14.241139676543888
12	33.05963597099547	22.653652560073148	27.51076581333925	16.775945655592135
13	22.451242544821955	33.48881427904043	28.583893361642254	15.476049814495358
14	17.935145177151934	35.76622234017607	29.239294627909135	17.05933785476286
15	16.657698942596266	24.7756402682319	42.33723126366745	16.229429525504386
16	18.336602311127915	22.97839929760907	39.95102895544798	18.73396943581503
17	19.0748944323158	22.488688852088902	29.964407830277338	28.472008885317955
18	22.605844895941075	23.4844697802847	33.7257621505087	20.183923173265516
19	25.685712779552134	26.893810634387705	27.308992021755213	20.111484564304945
20	27.59774667754912	25.28152897271691	26.64859185524639	20.472132494487578
21	23.029115412791004	28.30204627709177	28.779123138364106	19.88971517175312
22	22.479872609844016	25.452218693705564	27.93521788842818	24.132690808022236
23	19.648949958281907	29.850342015665642	27.361798586129233	23.138909439923218
24	19.448903059508726	25.705526602329304	35.09755126599421	19.74801907216776
25	18.702703587029035	27.48822531770283	32.84686459887916	20.962206496388976
26	18.148098327458868	30.499835490737492	29.38362651125843	21.968439670545205
27	19.25358239369157	29.223207258039597	29.843707111708152	21.679503236560684
28	17.391355719923435	28.224699656984555	35.080373226980974	19.30357139611104
29	18.442215439875955	25.563648724553325	33.31148965409428	22.68264618147644
30	19.80373408759164	28.284413792601992	30.957825641995125	20.954026477811244
31	25.01722348356089	27.420785608984204	26.231410907782106	21.3305799996728
32	25.941292915558577	27.567117052430284	26.144066487190997	20.347523544820138
33	24.251391966494644	27.898226026637772	25.953835610711096	21.896546396156484
34	19.156603728997805	28.907822097320405	28.929362812908433	23.006211360773356
35	20.791153219019087	28.160350177506405	29.53777441690101	21.5107221865735
36	25.599095471723494	27.3199896022875	26.37419767651117	20.706717249477844
37	21.470458317352	29.950320020504577	28.37539377700542	20.203827885137997
38	21.83319669672672	30.10728548810171	26.57160879152041	21.48790902365116
39	21.338396461869298	29.677652734580214	27.524944512207316	21.459006291343176
40	23.787494024041983	26.11988998783904	27.163751247452833	22.928864740666143
41	18.93928790211608	26.039362249396042	28.184981122334907	26.836368726152976
42	22.767173040112993	26.771019466626434	26.10034883234779	24.36145866091278
43	22.2514683133347	27.2864515261188	27.200015996480776	23.262064164065723
44	21.183612332559566	29.895604785129088	27.823696968485116	21.09708591382623
45	19.37037488116251	30.811403309453738	26.411825761968732	23.406396047415022
46	22.37362325742882	29.46406336060612	26.99542464294219	21.16688873902287
47	21.5621654145179	27.29699466117454	29.05288109343217	22.08795883087539
48	23.312780279247654	24.547145082627274	29.20348432435773	22.936590313767336
49	21.675867672748357	24.25693620130844	30.33814379018434	23.72905233575886
50	19.819094344698712	28.751401964295127	29.25247354672881	22.17703014427735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1062.0
1	1235.5
2	1409.0
3	5801.0
4	10193.0
5	7273.5
6	4354.0
7	4542.5
8	4731.0
9	5008.5
10	5286.0
11	5337.0
12	5388.0
13	5195.5
14	5003.0
15	4854.5
16	4706.0
17	4591.5
18	4477.0
19	4516.0
20	4555.0
21	4912.5
22	5270.0
23	5931.5
24	6593.0
25	7467.5
26	8342.0
27	10204.5
28	12067.0
29	13585.0
30	15103.0
31	18376.5
32	21650.0
33	24928.0
34	28206.0
35	31433.0
36	34660.0
37	37925.5
38	41191.0
39	47425.5
40	53660.0
41	63886.0
42	74112.0
43	85797.5
44	97483.0
45	101071.5
46	104660.0
47	105275.0
48	105890.0
49	102807.0
50	99724.0
51	89523.5
52	79323.0
53	70863.5
54	62404.0
55	54710.0
56	47016.0
57	42794.0
58	38572.0
59	34554.5
60	30537.0
61	27680.0
62	24823.0
63	21703.5
64	18584.0
65	16073.0
66	13562.0
67	10923.0
68	8284.0
69	7318.5
70	6353.0
71	5342.5
72	4332.0
73	3547.5
74	2763.0
75	2258.5
76	1754.0
77	1436.5
78	1119.0
79	835.0
80	551.0
81	413.0
82	275.0
83	199.0
84	123.0
85	98.0
86	73.0
87	42.5
88	12.0
89	6.0
90	0.0
91	1.0
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1100242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.173326275184145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30266215068959	20.49441124685511
2	6.535493211173904	3.4211119237061114
3	2.4370512184521935	1.9135721006965298
4	1.4262826202554055	1.493222415222772
5	0.9935562804804865	1.3002336350887074
6	0.7302639636767747	1.1468062192992867
7	0.6427911502865945	1.1776787752276374
8	0.5027256526921878	1.0526402027854032
9	0.44216944423417737	1.041574061957217
>10	6.724585671759792	43.720217126171676
>50	1.20408330576071	19.955482860264073
>100	0.05554082368609475	2.2107030349857637
>500	0.002095880139097781	0.3577535556616591
>1k	3.493133565162968E-4	0.16356276797309177
>5k	3.493133565162968E-4	0.5510300741049883
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6027	0.5477885774220581	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1789	0.16260059150623227	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05807813190189068	0.0
2	0.0	0.0	0.0	0.2334940858465683	0.0
3	0.0	0.0	0.0	0.385369764106442	0.0
4	0.0	0.0	0.0	0.6138649497110635	0.0
5	0.0	0.0	0.0	1.0306823407941161	0.0
6	0.0	0.0	0.0	1.7794267079424344	0.0
7	0.0	0.0	0.0	2.185882742160361	0.0
8	0.0	0.0	0.0	3.5105004171809475	0.0
9	0.0	0.0	0.0	4.250519431179686	0.0
10	0.0	0.0	0.0	5.2800202137347965	0.0
11	0.0	0.0	0.0	5.846259277504403	0.0
12	0.0	0.0	0.0	6.399682978835565	0.0
13	0.0	0.0	0.0	6.682711621625061	0.0
14	0.0	0.0	0.0	6.810229022342358	0.0
15	0.0	0.0	0.0	6.945108439779612	0.0
16	0.0	0.0	0.0	7.200779464881363	0.0
17	0.0	0.0	0.0	7.472992305329191	0.0
18	0.0	0.0	0.0	7.92534733267772	0.0
19	0.0	0.0	0.0	8.08276724575139	0.0
20	0.0	0.0	0.0	8.290721495816374	0.0
21	0.0	0.0	0.0	8.494494847497187	0.0
22	0.0	0.0	0.0	8.69581419360468	0.0
23	0.0	0.0	0.0	8.926399828401388	0.0
24	0.0	0.0	0.0	9.100816002297677	0.0
25	0.0	0.0	0.0	9.247056556648447	0.0
26	0.0	0.0	0.0	9.392933554617985	0.0
27	0.0	0.0	0.0	9.55680659345853	0.0
28	0.0	0.0	0.0	9.706319155240392	0.0
29	0.0	0.0	0.0	9.875827317990042	0.0
30	0.0	0.0	0.0	10.117319644223725	0.0
31	0.0	0.0	0.0	10.292462930882479	0.0
32	0.0	0.0	0.0	10.471514448639482	0.0
33	0.0	0.0	0.0	10.645748844345153	0.0
34	9.088909530812312E-5	0.0	0.0	10.82889037139102	0.0
35	9.088909530812312E-5	0.0	0.0	11.036026619598234	0.0
36	9.088909530812312E-5	0.0	0.0	11.21735036473794	0.0
37	9.088909530812312E-5	0.0	0.0	11.394674989684088	0.0
38	9.088909530812312E-5	0.0	0.0	11.598084784983667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.5276186E-6	44.0	1
GTCGACG	25	4.442715E-5	44.0	12
ACGTTAG	50	2.7284841E-11	44.0	1
CACGACG	30	2.5276186E-6	44.0	26
TATTCCG	20	7.856334E-4	44.0	1
GCGATCG	35	1.4460056E-7	44.0	9
TCGTAAG	35	1.4460056E-7	44.0	1
CCGCATA	20	7.856334E-4	44.0	19
AGTCGAC	25	4.442715E-5	44.0	11
CGAAGTA	20	7.856334E-4	44.0	38
TATAGCG	100	0.0	41.800003	1
CGTTTTT	3975	0.0	41.675472	1
AGTACGG	165	0.0	41.333332	2
TAGTACG	70	0.0	40.857143	1
AGGGCGA	1065	0.0	39.4554	6
TACGGGA	475	0.0	39.36842	4
CGTTAGG	190	0.0	39.36842	2
GCGATAC	90	0.0	39.11111	9
GAGTACG	85	0.0	38.823532	1
ACGGGCC	155	0.0	38.322582	5
>>END_MODULE