FastQCFastQC Report
Thu 26 May 2016
SRR1546496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546496_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1119442
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68970.61611052649445No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23640.2111766397901812No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.856394E-444.01
TCGTTAG254.4427663E-544.01
CTATCGG207.856394E-444.03
CGGCCTA254.4427663E-544.044
TAACGTA254.4427663E-544.023
TATAGCG254.4427663E-544.01
GCGTAAG750.044.01
CGTTTTT39350.041.37231
ATTAGCG1150.040.173911
TAGCCGT1000.039.644
CGAATAT1450.039.44827714
CGCATCG1250.038.7221
CGCCAGT1250.038.7226
ATGGTCG1200.038.50000444
GTTACGG1150.038.2608682
TACGAAT1500.038.1333312
GCTACGA1500.038.1333310
AGGGCGA12400.037.790326
CGGTTAT357.2900366E-637.7142875
TACACGC3400.037.5294135
Produced by FastQC (version 0.11.5)