##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546496_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1119442
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41755356686635	31.0	31.0	33.0	30.0	34.0
2	31.81038499538163	31.0	31.0	34.0	30.0	34.0
3	31.954313845648098	33.0	31.0	34.0	30.0	34.0
4	35.62721784603401	37.0	35.0	37.0	33.0	37.0
5	35.519663367999414	37.0	35.0	37.0	33.0	37.0
6	35.555047068092854	37.0	35.0	37.0	33.0	37.0
7	36.00104427027037	37.0	35.0	37.0	35.0	37.0
8	35.9923497599697	37.0	35.0	37.0	35.0	37.0
9	37.681292108032395	39.0	37.0	39.0	35.0	39.0
10	37.10011059081221	39.0	37.0	39.0	33.0	39.0
11	36.83036191245281	39.0	37.0	39.0	32.0	39.0
12	36.29563478947547	38.0	35.0	39.0	32.0	39.0
13	36.07367152563509	38.0	35.0	39.0	32.0	39.0
14	37.117280752374846	39.0	35.0	41.0	32.0	41.0
15	37.337266245147134	39.0	35.0	41.0	32.0	41.0
16	37.41777957232264	39.0	35.0	41.0	32.0	41.0
17	37.34378824450039	39.0	35.0	41.0	32.0	41.0
18	37.29958407849625	39.0	35.0	41.0	32.0	41.0
19	37.267054478927896	39.0	35.0	41.0	32.0	41.0
20	37.12131043859351	39.0	35.0	41.0	32.0	41.0
21	37.001846455644866	39.0	35.0	41.0	32.0	41.0
22	36.934846110830215	38.0	35.0	41.0	32.0	41.0
23	36.83907964861065	38.0	35.0	41.0	32.0	41.0
24	36.77150223057559	38.0	35.0	41.0	31.0	41.0
25	36.588312748673	38.0	35.0	40.0	31.0	41.0
26	36.48690508306817	38.0	35.0	40.0	31.0	41.0
27	36.410129332292335	38.0	35.0	40.0	31.0	41.0
28	36.48079132281976	38.0	35.0	40.0	31.0	41.0
29	36.410163277775894	38.0	35.0	40.0	31.0	41.0
30	36.38929573841253	38.0	35.0	40.0	31.0	41.0
31	36.280622846025075	38.0	35.0	40.0	31.0	41.0
32	36.10023654642224	38.0	35.0	40.0	30.0	41.0
33	36.030599173516805	38.0	35.0	40.0	30.0	41.0
34	35.91799485815255	38.0	35.0	40.0	30.0	41.0
35	35.756981603334516	38.0	35.0	40.0	29.0	41.0
36	35.58424733036638	38.0	35.0	40.0	29.0	41.0
37	35.5276896882554	38.0	35.0	40.0	28.0	41.0
38	35.44241506036043	38.0	34.0	40.0	28.0	41.0
39	35.35648206874497	38.0	34.0	40.0	28.0	41.0
40	35.22293785653924	37.0	34.0	40.0	27.0	41.0
41	35.22116822488347	37.0	34.0	40.0	27.0	41.0
42	35.04501796430722	37.0	34.0	40.0	27.0	41.0
43	35.05682831267721	37.0	34.0	40.0	27.0	41.0
44	34.8154339394091	37.0	34.0	40.0	26.0	41.0
45	34.709274799408995	36.0	34.0	40.0	26.0	41.0
46	34.69616916285078	36.0	34.0	40.0	26.0	41.0
47	34.57833545641489	36.0	34.0	40.0	26.0	41.0
48	34.550853014269606	36.0	34.0	40.0	26.0	41.0
49	34.52624343199558	36.0	34.0	40.0	26.0	41.0
50	33.9908909974791	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	6.0
13	7.0
14	10.0
15	32.0
16	71.0
17	179.0
18	355.0
19	827.0
20	1442.0
21	2389.0
22	3543.0
23	5162.0
24	7478.0
25	10533.0
26	14296.0
27	18038.0
28	20279.0
29	22934.0
30	26970.0
31	33113.0
32	41567.0
33	54181.0
34	90799.0
35	143544.0
36	86854.0
37	108605.0
38	158669.0
39	267557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.838108629120576	23.851258037486534	28.270602675261426	14.04003065813146
2	31.923762017147826	25.40176266389862	28.97899131888923	13.695484000064317
3	29.61546913551573	25.104203701486995	31.108534430546648	14.17179273245063
4	25.84073136437618	28.800420209354304	30.269812996117707	15.089035430151807
5	23.13000584219638	32.52236382054631	29.29700690165279	15.050623435604523
6	20.324054305627268	41.21213961956046	26.957448443063598	11.506357631748674
7	85.75397385483124	4.601667616544671	7.391182392656341	2.253176135967741
8	85.64123911734598	3.7668767117903386	6.945960576787363	3.645923594076334
9	79.01061421672584	5.977799653756068	10.344886112902678	4.666700016615421
10	45.61352888313999	25.350665778128743	15.434207399758094	13.601597938973168
11	37.00013042212102	23.511356550853012	24.239308512633972	15.249204514391993
12	33.36662372860765	20.865931419403598	27.541221429962427	18.22622342202633
13	23.07462110587239	30.759789252145264	28.720648323003783	17.444941318978564
14	18.540844456434545	34.119230831074766	29.340153397853573	17.999771314637115
15	17.679790467036256	22.342023972657806	41.8761311439092	18.10205441639674
16	19.902772988685435	19.909651415615993	39.46180329128262	20.72577230441595
17	19.616558964198237	21.08425447678397	29.50443167220812	29.794754886809677
18	23.239167370886566	22.263145388506057	32.60526226459254	21.892424976014834
19	27.702105155961632	25.605167574559466	25.754170381314978	20.938556888163927
20	29.206336728477222	23.694304841161937	24.80869933413254	22.2906590962283
21	23.708776336782076	27.91703366498666	27.15308162459511	21.221108373636152
22	24.135596127356308	23.915575795798265	26.11015130752643	25.838676769319
23	21.527779018475275	28.798186953857368	25.079637891020706	24.59439613664665
24	21.957100055206073	23.877074471031104	33.54188962000711	20.62393585375571
25	20.996621531084237	24.6511208262688	31.330698687381748	23.021558955265213
26	19.3996651903359	28.407545902333485	28.260240369755646	23.93254853757497
27	21.46712379917852	27.023820796432506	27.840477666551728	23.668577737837243
28	19.554206470723805	26.81434143082	33.20761593722587	20.423836161230327
29	20.930606498594837	23.850811386387146	32.3409341439753	22.877647971042716
30	21.609337509223344	26.165000062531153	30.731560902664008	21.494101525581495
31	27.28725561485097	24.524271914042888	25.6694853328712	22.51898713823494
32	28.015654227731318	25.30984186764477	26.286489161564425	20.388014743059486
33	26.085764157499895	24.66469901969017	26.092285263550945	23.157251559258988
34	21.454617568395683	26.469526782093222	29.132460636638612	22.943395012872482
35	22.131829965286276	25.007012422260377	29.43913128147774	23.422026330975612
36	27.727117617527302	25.755956985712526	25.561127776159907	20.955797620600265
37	24.14345718670552	27.50289876563502	26.360543913842786	21.99310013381667
38	23.9810548469684	29.688541255375444	23.820349781408954	22.510054116247204
39	23.28678037808122	27.122620019616917	24.6993591450026	24.89124045729926
40	25.002009929947246	24.62065922129061	25.902547876531344	24.47478297223081
41	20.47198514974425	23.14036814770216	27.69040289715769	28.6972438053959
42	23.88055834960632	25.317434936334354	25.46849233814704	25.333514375912287
43	22.984308253576337	24.555269500340348	26.914748597962202	25.54567364812112
44	22.75124570991619	27.1657665158177	27.3013697895916	22.781617984674508
45	21.06692441412775	28.55485143491132	26.389218914423434	23.98900523653749
46	24.31541785996952	27.659941292179496	26.153833785046476	21.870807062804502
47	23.00405023216924	25.260442256052567	27.72524168290988	24.01026582886831
48	23.95738233870089	23.040050310779836	28.225669574663094	24.776897775856185
49	23.34806090891712	22.44305645133915	29.276460951080985	24.932421688662746
50	21.167510241709707	26.556802406913445	28.323396835208968	23.952290516167878
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1302.0
1	1391.5
2	1481.0
3	6185.5
4	10890.0
5	7488.0
6	4086.0
7	4294.0
8	4502.0
9	4752.5
10	5003.0
11	5063.0
12	5123.0
13	4836.5
14	4550.0
15	4479.0
16	4408.0
17	4206.0
18	4004.0
19	4041.5
20	4079.0
21	4294.5
22	4510.0
23	4783.0
24	5056.0
25	5674.0
26	6292.0
27	6956.5
28	7621.0
29	8799.5
30	9978.0
31	11746.5
32	13515.0
33	14747.5
34	15980.0
35	20184.5
36	24389.0
37	27835.5
38	31282.0
39	35054.0
40	38826.0
41	52388.5
42	65951.0
43	76236.5
44	86522.0
45	93166.0
46	99810.0
47	102558.5
48	105307.0
49	103609.0
50	101911.0
51	92999.0
52	84087.0
53	76435.0
54	68783.0
55	62733.5
56	56684.0
57	53461.0
58	50238.0
59	48465.5
60	46693.0
61	43192.5
62	39692.0
63	36228.5
64	32765.0
65	27919.5
66	23074.0
67	19748.5
68	16423.0
69	14481.5
70	12540.0
71	10717.0
72	8894.0
73	7137.0
74	5380.0
75	4653.5
76	3927.0
77	3033.0
78	2139.0
79	1580.0
80	1021.0
81	693.5
82	366.0
83	287.0
84	208.0
85	153.5
86	99.0
87	63.0
88	27.0
89	15.5
90	4.0
91	4.5
92	5.0
93	7.0
94	9.0
95	6.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1119442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.16506865169289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.96068592932231	19.618860135444635
2	6.678528695505093	3.361312662293732
3	2.412237933552796	1.8211239960622172
4	1.4556868102819798	1.4652983406443947
5	1.0227197894863478	1.2868406856934422
6	0.7953087798488505	1.2008400026514259
7	0.6437458985986698	1.1339936812736877
8	0.5203904163169233	1.0476528441838735
9	0.4403674558324534	0.9973689532195523
>10	6.582733356745648	40.92929940484357
>50	1.411271440937338	23.097596417916467
>100	0.07382693069780451	2.867296926136348
>500	0.0017832591955990896	0.3413256396561904
>1k	3.566518391198179E-4	0.2121729719030329
>5k	3.566518391198179E-4	0.6190173380775034
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6897	0.61611052649445	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2364	0.2111766397901812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06235249347442744	0.0
2	0.0	0.0	0.0	0.2511965782952578	0.0
3	0.0	0.0	0.0	0.40698848176144903	0.0
4	0.0	0.0	0.0	0.6362098259668656	0.0
5	0.0	0.0	0.0	1.0786624050196436	0.0
6	0.0	0.0	0.0	1.9109520636174093	0.0
7	0.0	0.0	0.0	2.403340235581656	0.0
8	0.0	0.0	0.0	3.93124431636476	0.0
9	0.0	0.0	0.0	4.825261156897811	0.0
10	0.0	0.0	0.0	6.070345761549057	0.0
11	0.0	0.0	0.0	6.705036973778007	0.0
12	0.0	0.0	0.0	7.317574291477361	0.0
13	0.0	0.0	0.0	7.636751167099323	0.0
14	0.0	0.0	0.0	7.763868069984867	0.0
15	0.0	0.0	0.0	7.889109038252987	0.0
16	0.0	0.0	0.0	8.144325476442727	0.0
17	0.0	0.0	0.0	8.414549391571873	0.0
18	0.0	0.0	0.0	8.882997064608976	0.0
19	0.0	0.0	0.0	9.044595432367197	0.0
20	0.0	0.0	0.0	9.253985467759831	0.0
21	0.0	0.0	0.0	9.462660861393445	0.0
22	0.0	0.0	0.0	9.664189837436865	0.0
23	0.0	0.0	0.0	9.890641944826083	0.0
24	0.0	0.0	0.0	10.07064233787905	0.0
25	0.0	0.0	0.0	10.21723322869787	0.0
26	0.0	0.0	0.0	10.359714929402328	0.0
27	0.0	0.0	0.0	10.526762440573071	0.0
28	0.0	0.0	0.0	10.664598969843905	0.0
29	0.0	0.0	0.0	10.817353645834263	0.0
30	0.0	0.0	0.0	11.043180441684338	0.0
31	0.0	0.0	0.0	11.214873124288708	0.0
32	0.0	0.0	0.0	11.379151398643252	0.0
33	0.0	0.0	0.0	11.54610957959412	0.0
34	0.0	0.0	0.0	11.721732791873094	0.0
35	0.0	0.0	0.0	11.916829992085342	0.0
36	0.0	0.0	0.0	12.085038796114492	0.0
37	0.0	0.0	0.0	12.256374157839351	0.0
38	0.0	0.0	0.0	12.44182369430484	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.856394E-4	44.0	1
TCGTTAG	25	4.4427663E-5	44.0	1
CTATCGG	20	7.856394E-4	44.0	3
CGGCCTA	25	4.4427663E-5	44.0	44
TAACGTA	25	4.4427663E-5	44.0	23
TATAGCG	25	4.4427663E-5	44.0	1
GCGTAAG	75	0.0	44.0	1
CGTTTTT	3935	0.0	41.3723	1
ATTAGCG	115	0.0	40.17391	1
TAGCCGT	100	0.0	39.6	44
CGAATAT	145	0.0	39.448277	14
CGCATCG	125	0.0	38.72	21
CGCCAGT	125	0.0	38.72	26
ATGGTCG	120	0.0	38.500004	44
GTTACGG	115	0.0	38.260868	2
TACGAAT	150	0.0	38.13333	12
GCTACGA	150	0.0	38.13333	10
AGGGCGA	1240	0.0	37.79032	6
CGGTTAT	35	7.2900366E-6	37.714287	5
TACACGC	340	0.0	37.52941	35
>>END_MODULE