FastQCFastQC Report
Thu 26 May 2016
SRR1546495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546495_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1365852
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158741.162204982677479No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC18680.13676445178540575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91800.042.130721
ATAACGC1550.039.74193611
TATCGCG452.3514076E-839.111111
TAGCCGT2200.039.044
TCGTTTA1500.038.1333338
CGCATCG1500.038.1333321
TTTAAGC13100.038.1221444
TATACTA6100.037.86885544
AACCCGA2500.037.8424
ACCCGCT13400.037.76119634
CGACCCG13550.037.66789632
GTTTTTT107050.037.2592282
CATATGC11850.036.9451533
ATACGAA2150.036.8372119
GCTGAAT13750.036.838
ACGTGGC13900.036.71942526
AACGTAG601.9826984E-1036.6666681
TATAGCG900.036.6666681
ACATACG2100.036.66666817
ATTATAC6550.036.61068742
Produced by FastQC (version 0.11.5)