FastQCFastQC Report
Thu 26 May 2016
SRR1546494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546494_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2050068
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204850.9992351473219425No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21740.10604526288884077No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC21560.10516724323290741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113800.041.9314581
TCGTAAG950.039.368421
GCGTAAG1300.038.923081
AGGGTAC12150.038.7489746
TCGTTTA2350.038.3829838
TACGGGT1150.038.260874
CATATGC20350.037.40540733
CGGTCTA1900.037.0526331
GGTACCT22300.036.9955188
CATACGA2800.036.92857418
GTTGATC21400.036.80373816
TCAGACG19700.036.62944422
TATGGGA12000.036.4833344
GCGCGAC19900.036.4824149
ATATGCT20750.036.4722934
TAGCATA20750.036.4722930
ACCCGCT18600.036.4301134
TTTAAGC18650.036.33244344
CAGTAGC21400.036.18691627
GTTTTTT138600.036.111112
Produced by FastQC (version 0.11.5)