##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546494_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2050068
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36855314067631	31.0	31.0	33.0	30.0	34.0
2	31.765237055551328	31.0	31.0	34.0	30.0	34.0
3	31.891087515145838	31.0	31.0	34.0	30.0	34.0
4	35.58729612871378	37.0	35.0	37.0	33.0	37.0
5	35.48417223233571	37.0	35.0	37.0	33.0	37.0
6	35.51276543021987	37.0	35.0	37.0	33.0	37.0
7	35.96888103223893	37.0	35.0	37.0	35.0	37.0
8	35.9579613944513	37.0	35.0	37.0	35.0	37.0
9	37.65102084418663	39.0	37.0	39.0	35.0	39.0
10	37.04123668092961	39.0	37.0	39.0	33.0	39.0
11	36.80231631341009	39.0	37.0	39.0	32.0	39.0
12	36.388210537406565	39.0	35.0	39.0	32.0	39.0
13	36.249610744619204	39.0	35.0	39.0	32.0	39.0
14	37.27372604225811	39.0	35.0	41.0	32.0	41.0
15	37.42574051202204	39.0	35.0	41.0	32.0	41.0
16	37.470903404179765	39.0	35.0	41.0	32.0	41.0
17	37.38733349332803	39.0	35.0	41.0	32.0	41.0
18	37.3088560964807	39.0	35.0	41.0	32.0	41.0
19	37.25377402115442	39.0	35.0	41.0	32.0	41.0
20	37.07715744063124	39.0	35.0	41.0	32.0	41.0
21	36.96198906572855	39.0	35.0	41.0	31.0	41.0
22	36.896979027037155	38.0	35.0	41.0	31.0	41.0
23	36.805189388839786	38.0	35.0	41.0	31.0	41.0
24	36.72376379710332	38.0	35.0	41.0	31.0	41.0
25	36.541202535720764	38.0	35.0	41.0	31.0	41.0
26	36.4386825217505	38.0	35.0	40.0	31.0	41.0
27	36.35486481424031	38.0	35.0	40.0	30.0	41.0
28	36.38474674986391	38.0	35.0	40.0	31.0	41.0
29	36.251712626117765	38.0	35.0	40.0	30.0	41.0
30	36.1947715880644	38.0	35.0	40.0	30.0	41.0
31	36.047917434933865	38.0	35.0	40.0	30.0	41.0
32	35.80156560660427	38.0	35.0	40.0	29.0	41.0
33	35.66308580983655	38.0	35.0	40.0	28.0	41.0
34	35.44587594167608	38.0	34.0	40.0	27.0	41.0
35	35.22147021464654	38.0	34.0	40.0	26.0	41.0
36	35.03979819205997	38.0	34.0	40.0	25.0	41.0
37	35.02589816532915	38.0	34.0	40.0	25.0	41.0
38	34.958795025335746	37.0	34.0	40.0	25.0	41.0
39	34.84241205657568	37.0	34.0	40.0	24.0	41.0
40	34.70415908155242	37.0	34.0	40.0	24.0	41.0
41	34.65884936499668	37.0	34.0	40.0	24.0	41.0
42	34.47858363722569	37.0	33.0	40.0	23.0	41.0
43	34.467064994917244	37.0	33.0	40.0	23.0	41.0
44	34.195052066565594	36.0	33.0	40.0	23.0	41.0
45	34.083901607166204	36.0	33.0	40.0	23.0	41.0
46	34.067392886479865	36.0	33.0	40.0	23.0	41.0
47	33.95579805157683	35.0	33.0	40.0	23.0	41.0
48	33.88150588175612	35.0	33.0	40.0	23.0	41.0
49	33.8505049588599	35.0	33.0	40.0	23.0	41.0
50	33.34889086605908	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	2.0
13	11.0
14	26.0
15	74.0
16	182.0
17	409.0
18	941.0
19	1958.0
20	3277.0
21	5179.0
22	7950.0
23	11477.0
24	16613.0
25	23822.0
26	33372.0
27	40816.0
28	44898.0
29	47844.0
30	53914.0
31	63793.0
32	77191.0
33	99070.0
34	157901.0
35	222000.0
36	166772.0
37	212222.0
38	299419.0
39	458928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.16252534062284	23.208547228677293	26.157278685389944	16.47164874530991
2	34.738506234915135	24.38553257745597	27.67527711275919	13.200684074869711
3	29.689844434428515	24.751130206412668	31.619195070602537	13.939830288556282
4	25.71290318174812	27.020128112823578	32.31556221549724	14.951406489931065
5	22.626566533402794	31.904746574259974	30.427527282021867	15.041159610315367
6	20.295082894811294	40.273298251570196	28.394033758880195	11.03758509473832
7	84.39076167229574	4.117229282150641	9.206182429070646	2.2858266164829657
8	84.01628628904017	3.5917832969442967	9.069504035963686	3.32242637805185
9	78.36988821834203	5.09236766780419	12.111793364903017	4.425950748950767
10	43.2036888532478	22.445499368801425	18.14076411123924	16.210047666711542
11	34.7863583061635	23.93398657995735	24.56377056761044	16.715884546268708
12	31.29593750060974	19.70178550174921	28.990550557347365	20.011726440293685
13	24.748886378403057	24.609915378416716	30.91492574880443	19.726272494375795
14	20.987547730124074	27.66225315452951	31.658657176249765	19.691541939096656
15	19.81270865161546	21.42480151877889	38.700813826663314	20.06167600294234
16	22.692418007597798	19.338236585323024	36.52561768682795	21.44372772025123
17	22.26965154326588	19.963484138087125	31.251109719287363	26.51575459935963
18	24.399288218732256	21.132420973353078	32.23327226218838	22.235018545726287
19	28.250770218353733	24.702839125336332	26.664432594431016	20.381958061878926
20	28.86577420846528	23.92242598782089	25.926749746837668	21.285050056876162
21	25.941968754207178	25.87319054782573	27.956340960397412	20.22849973756968
22	24.534991034443735	22.58998238107224	27.64747315698796	25.227553427496062
23	23.4047846217784	25.946651525705487	26.237763820517173	24.41080003199894
24	23.590680894487402	23.63028933674395	31.040872790561092	21.738156978207552
25	24.462115403001267	23.238253560369703	28.82475117898528	23.474879857643746
26	21.03081458761368	25.886897410232244	28.254428633586787	24.82785936856729
27	22.864461081290962	23.465172862558706	28.864554736720926	24.805811319429406
28	22.729977737323832	24.87483342015972	30.72376135815983	21.671427484356617
29	23.88901246202565	24.0196910541504	30.082270441760954	22.009026042062995
30	25.43808302944097	23.39522396330268	29.379757159274718	21.786935847981628
31	28.532516970168793	23.130110806080577	25.678172626468974	22.659199597281653
32	29.66964998234205	23.284105697957337	25.771974393044523	21.274269926656093
33	29.012793721964346	23.26605751614093	24.80288458724296	22.918264174651767
34	25.370963304631843	25.102142953306917	27.95370690142961	21.573186840631628
35	25.28408813756422	24.792641024590402	27.93995126015332	21.983319577692058
36	28.311841363310876	25.684855331628025	24.796494555302555	21.206808749758544
37	25.997820560098496	27.9700966016737	25.01292640049013	21.019156437737674
38	26.53336377134807	27.961462741723693	23.728968990296906	21.776204496631333
39	24.783177923854232	26.682919786075388	24.062518901812037	24.471383388258342
40	25.528519054002114	24.502455528304427	27.115295687752795	22.853729729940667
41	22.60725010097226	24.0354954079572	27.258071439581517	26.09918305148902
42	24.74449628012339	26.013966365993713	24.729667503712072	24.51186985017082
43	23.700433351479074	24.557819545498003	26.750137068624063	24.99161003439886
44	24.279243420218254	24.6833275774267	27.92200063607646	23.115428366278582
45	22.454718575188725	26.406197257847058	27.247583982580093	23.891500184384128
46	24.72293601968325	25.51451951837695	26.904375854849693	22.858168607090107
47	23.457026791306433	25.373743700208966	27.267778434666557	23.901451073818038
48	23.96140030477038	24.505626154839742	27.823028309304863	23.709945231085015
49	24.62098818185543	23.174792250793633	27.927073638532967	24.27714592881797
50	22.96328707145324	24.28826751112646	27.988339898969205	24.760105518451095
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1878.0
1	2339.5
2	2801.0
3	17595.5
4	32390.0
5	22095.0
6	11800.0
7	11958.5
8	12117.0
9	12543.0
10	12969.0
11	12851.0
12	12733.0
13	12176.5
14	11620.0
15	11164.0
16	10708.0
17	10244.0
18	9780.0
19	9201.5
20	8623.0
21	8460.5
22	8298.0
23	8416.5
24	8535.0
25	9336.5
26	10138.0
27	11201.0
28	12264.0
29	14433.5
30	16603.0
31	19062.0
32	21521.0
33	24788.5
34	28056.0
35	32737.0
36	37418.0
37	41280.5
38	45143.0
39	52678.0
40	60213.0
41	74039.0
42	87865.0
43	100754.5
44	113644.0
45	123943.5
46	134243.0
47	147603.0
48	160963.0
49	164500.5
50	168038.0
51	156338.0
52	144638.0
53	134335.5
54	124033.0
55	120909.0
56	117785.0
57	118143.5
58	118502.0
59	116445.5
60	114389.0
61	106746.5
62	99104.0
63	92419.0
64	85734.0
65	73561.0
66	61388.0
67	52951.0
68	44514.0
69	40375.0
70	36236.0
71	31266.0
72	26296.0
73	21487.0
74	16678.0
75	13426.0
76	10174.0
77	7438.0
78	4702.0
79	3775.0
80	2848.0
81	2172.5
82	1497.0
83	1122.0
84	747.0
85	502.5
86	258.0
87	190.5
88	123.0
89	79.5
90	36.0
91	26.0
92	16.0
93	11.0
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2050068.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.182288453473916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.97253409194661	20.153170905902016
2	6.5339821533984415	3.4214921098025735
3	2.6169016344604707	2.0554942034343426
4	1.5608702189316683	1.6346861724202373
5	1.1115162984249105	1.4551020173049303
6	0.8346497692366686	1.311182460946794
7	0.6950736356655127	1.2739032897779503
8	0.587420955891043	1.2304019928603736
9	0.4881684238283275	1.1503229837895883
>10	7.742067298804625	48.787659511149215
>50	0.79874029666021	12.99279867955924
>100	0.05264243504885231	2.367918097038411
>500	0.004683437674763897	0.8798944322784851
>1k	5.620125209716677E-4	0.28119932996558084
>5k	0.0	0.0
>10k+	1.873375069905559E-4	1.004773813770264
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20485	0.9992351473219425	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2174	0.10604526288884077	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC	2156	0.10516724323290741	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04677893611333868	0.0
2	0.0	0.0	0.0	0.1688236682880763	0.0
3	0.0	0.0	0.0	0.30979460193515534	0.0
4	0.0	0.0	0.0	0.4470583414794046	0.0
5	0.0	0.0	0.0	0.702220609267595	0.0
6	0.0	0.0	0.0	1.3826858426159523	0.0
7	0.0	0.0	0.0	1.811256992450982	0.0
8	0.0	0.0	0.0	3.072581007069034	0.0
9	0.0	0.0	0.0	3.9090410659548853	0.0
10	0.0	0.0	0.0	5.034515928252136	0.0
11	0.0	0.0	0.0	5.5948875842167185	0.0
12	0.0	0.0	0.0	6.076237471147299	0.0
13	0.0	0.0	0.0	6.374910490773964	0.0
14	0.0	0.0	0.0	6.534612510414289	0.0
15	0.0	0.0	0.0	6.649389190992689	0.0
16	0.0	0.0	0.0	6.816261704489802	0.0
17	0.0	0.0	0.0	6.97708563813493	0.0
18	0.0	0.0	0.0	7.264344402234462	0.0
19	0.0	0.0	0.0	7.381755141780663	0.0
20	0.0	0.0	0.0	7.5342866675641975	0.0
21	0.0	0.0	0.0	7.66823344396381	0.0
22	0.0	0.0	0.0	7.819252824784349	0.0
23	0.0	0.0	0.0	7.984564414448691	0.0
24	0.0	0.0	0.0	8.133681419348042	0.0
25	0.0	0.0	0.0	8.253531102382945	0.0
26	0.0	0.0	0.0	8.366502964779706	0.0
27	0.0	0.0	0.0	8.51088841931097	0.0
28	0.0	0.0	0.0	8.634201402099833	0.0
29	0.0	0.0	0.0	8.775123556877137	0.0
30	0.0	0.0	0.0	8.972482863983048	0.0
31	0.0	0.0	0.0	9.119502377482112	0.0
32	0.0	0.0	0.0	9.276863011373282	0.0
33	0.0	0.0	0.0	9.43446753961332	0.0
34	0.0	0.0	0.0	9.588998999057592	0.0
35	0.0	0.0	0.0	9.759822601006405	0.0
36	0.0	0.0	0.0	9.908500596077788	0.0
37	0.0	0.0	0.0	10.065519777880539	0.0
38	0.0	0.0	0.0	10.273122647639005	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11380	0.0	41.931458	1
TCGTAAG	95	0.0	39.36842	1
GCGTAAG	130	0.0	38.92308	1
AGGGTAC	1215	0.0	38.748974	6
TCGTTTA	235	0.0	38.38298	38
TACGGGT	115	0.0	38.26087	4
CATATGC	2035	0.0	37.405407	33
CGGTCTA	190	0.0	37.05263	31
GGTACCT	2230	0.0	36.995518	8
CATACGA	280	0.0	36.928574	18
GTTGATC	2140	0.0	36.803738	16
TCAGACG	1970	0.0	36.629444	22
TATGGGA	1200	0.0	36.483334	4
GCGCGAC	1990	0.0	36.482414	9
ATATGCT	2075	0.0	36.47229	34
TAGCATA	2075	0.0	36.47229	30
ACCCGCT	1860	0.0	36.43011	34
TTTAAGC	1865	0.0	36.332443	44
CAGTAGC	2140	0.0	36.186916	27
GTTTTTT	13860	0.0	36.11111	2
>>END_MODULE