FastQCFastQC Report
Thu 26 May 2016
SRR1546493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546493_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences977175
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92850.9501880420600199No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14340.14674955867679793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG900.044.02
TCGATAG600.044.01
TCGACAG207.8559027E-444.01
CGTTTTT48000.042.3041651
ATGGTCG750.041.06666644
CGTAAGG2050.039.7073172
TATACTA3050.039.6721344
GTTGATC6900.038.8985516
ACGGGAT1500.038.133335
TATGCTT6950.037.66906735
GCCAGTA7150.036.92307725
AGGGCGA10850.036.9032256
CATATGC7100.036.8732433
TAGGGCG4900.036.8163265
TAGCATA7000.036.77142730
TCGTTTA900.036.66666838
TACGAAT301.3009527E-436.66666412
GCCCGTT301.3009527E-436.66666444
GTACGGG3550.036.563383
AGGGTAC5000.036.526
Produced by FastQC (version 0.11.5)